(Circular) (Six-base) MAPSORT of: pFN21AE4328.seq Check: 732 from: 1 to: 5707 pFN21AE4328 5707 bp With 182 enzymes: SgfI * March 28, 2012 13:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 2252 2376 3028 2252 Size: 124 652 4931 Fragments arranged by size: 4931 652 124 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2779 820 Size: 197 1762 3748 Fragments arranged by size: 3748 1762 197 AflIII A'CryG_T Cuts at: 1203 2246 4606 5583 1203 Size: 1043 2360 977 1327 Fragments arranged by size: 2360 1327 1043 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 2039 Size: 32 5675 AlwNI CAG_nnn'CTG Cuts at: 2651 3450 5022 2651 Size: 799 1572 3336 Fragments arranged by size: 3336 1572 799 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2350 2389 2461 2538 2628 2750 2840 3016 Size: 857 39 72 77 90 122 90 176 Cuts at: 3016 3242 3394 5503 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 857 226 176 152 122 90 90 77 72 39 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2376 3028 4483 618 Size: 325 217 627 465 124 652 1455 1842 Fragments arranged by size: 1842 1455 652 627 465 325 217 124 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2249 2818 928 Size: 971 350 569 3817 Fragments arranged by size: 3817 971 569 350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 2805 1548 Size: 1257 4450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2114 5702 2114 Size: 3588 2119 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2724 4367 1239 Size: 827 658 1643 2579 Fragments arranged by size: 2579 1643 827 658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 4140 4538 4812 4959 5370 2385 Size: 1755 398 274 147 411 2722 Fragments arranged by size: 2722 1755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 1178 Size: 30 165 30 636 30 4816 Fragments arranged by size: 4816 636 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2560 4910 5406 2560 Size: 2350 496 2861 Fragments arranged by size: 2861 2350 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1998 1163 Size: 573 262 4872 Fragments arranged by size: 4872 573 262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2221 2221 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3768 3853 4924 1391 Size: 399 1236 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 2355 3596 5326 2355 Size: 1241 1730 2736 Fragments arranged by size: 2736 1730 1241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2153 4202 4376 65 Size: 1183 905 2049 174 1396 Fragments arranged by size: 2049 1396 1183 905 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 2616 3767 4779 2616 Size: 1151 1012 3544 Fragments arranged by size: 3544 1151 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2025 4511 2025 Size: 2486 3221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2090 2090 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2087 2095 2114 3037 3404 3789 3806 5258 Size: 115 8 19 923 367 385 17 1452 Cuts at: 5258 5344 5356 5702 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 923 385 367 346 115 86 19 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2662 2697 2839 2874 529 Size: 35 2098 35 142 35 3362 Fragments arranged by size: 3362 2098 142 35 35 35 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672 Size: 54 1425 50 275 18 1324 889 1629 Cuts at: 5672 8 Size: 43 Fragments arranged by size: 1629 1425 1324 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2724 3770 4367 5154 5422 1239 Size: 393 434 658 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 787 658 597 434 393 268 EcoRI G'AATT_C Cuts at: 2461 2628 3016 3394 2461 Size: 167 388 378 4774 Fragments arranged by size: 4774 388 378 167 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 2187 2291 2329 748 Size: 1439 104 38 4126 Fragments arranged by size: 4126 1439 104 38 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 2256 2380 3032 2256 Size: 124 652 4931 Fragments arranged by size: 4931 652 124 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 3665 4024 4183 518 Size: 648 996 1503 359 159 2042 Fragments arranged by size: 2042 1503 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3123 3800 4948 5193 1606 Size: 1517 677 1148 245 2120 Fragments arranged by size: 2120 1517 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2215 2250 3065 4610 1132 Size: 97 986 35 815 1545 2229 Fragments arranged by size: 2229 1545 986 815 97 35 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 2246 4606 2246 Size: 2360 3347 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2071 3776 3808 5289 5321 2039 Size: 32 1705 32 1481 32 2425 Fragments arranged by size: 2425 1705 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SexAI A'CCwGG_T Cuts at: 2644 2644 Size: 5707 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2779 3811 4712 4974 820 Size: 197 926 836 1032 901 262 1553 Fragments arranged by size: 1553 1032 926 901 836 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 2371 2532 3146 513 Size: 552 939 79 288 161 614 3074 Fragments arranged by size: 3074 939 614 552 288 161 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 3734 3751 3904 4089 4534 325 Size: 1255 468 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 468 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2194 3953 5665 Size: 274 80 33 51 1443 217 1759 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1759 1712 1443 274 217 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 5667 5699 2829 Size: 32 2806 32 2837 Fragments arranged by size: 2837 2806 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2118 2118 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI