################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KspI MamI MluI MluNI MroI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 1152 GTATAC GT^[AC][GT]AC AccII 39 CGCG CG^CG AcyI 34 GGCGTC G[GA]^CG[CT]C Alw44I 694 GTGCAC G^TGCAC ApaI 253 GGGCCC GGGCC^C ApaLI 694 GTGCAC G^TGCAC AspHI 694 GTGCAC G[AT]GC[AT]^C BamHI 506 GGATCC G^GATCC BbvCI 9 GCTGAGG CCTCAGC(-5/-2) BcgI 967 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 34 GGCGTC G[GA]^CG[CT]C BsgI 346 CTGCAC GTGCAG(16/14) BsiHKAI 694 GTGCAC G[AT]GC[AT]^C BsmAI 1089 GTCTC GTCTC(1/5) BsmI 662 GAATGC GAATGC(1/-1) BspDI 1259 ATCGAT AT^CGAT BspLU11I 1325 ACATGT A^CATGT BspMI 902 ACCTGC ACCTGC(4/8) BsrFI 410 ACCGGC [GA]^CCGG[CT] Bst1107I 1152 GTATAC GTA^TAC BstUI 39 CGCG CG^CG BstXI 1290 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1152 GTATAC GTA^TAC Cfr10I 410 ACCGGC [GA]^CCGG[CT] ClaI 1259 ATCGAT AT^CGAT DsaI 1121 CCATGG C^C[GA][CT]GG EaeI 682 CGGCCA [CT]^GGCC[GA] EarI 755 CTCTTC CTCTTC(1/4) EcoT22I 664 ATGCAT ATGCA^T HaeII 6 GGCGCT [GA]GCGC^[CT] HgaI 35 GCGTC GACGC(5/10) Hin1I 34 GGCGTC G[GA]^CG[CT]C Ksp632I 755 CTCTTC CTCTTC(1/4) MaeII 534 ACGT A^CGT MvnI 39 CGCG CG^CG NcoI 1121 CCATGG C^CATGG NsiI 664 ATGCAT ATGCA^T PaeR7I 965 CTCGAG C^TCGAG PflMI 546 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SmlI 965 CTCGAG C^T[CT][GA]AG SpeI 288 ACTAGT A^CTAGT SphI 666 GCATGC GCATG^C StuI 446 AGGCCT AGG^CCT Van91I 546 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 965 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 986 GGTACC G^GTACC 1354 GGTACC AflIII 532 ACACGT A^C[GA][CT]GT 1325 ACATGT AhdI 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1306 GACAAGATGTC AlwNI 46 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1015 CAGTCCCTG Asp718I 986 GGTACC G^GTACC 1354 GGTACC AspEI 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1306 GACAAGATGTC AvaI 494 CTCGGG C^[CT]CG[GA]G 965 CTCGAG BanI 986 GGTACC G^G[CT][GA]CC 1354 GGTACC BbsI 909 GAAGAC GAAGAC(2/6) 1113 GAAGAC BcnI 30 CCGGG CC^[GC]GG 996 CCGGG BglI 1195 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1288 GCCCATCTGGC BpuAI 909 GAAGAC GAAGAC(2/6) 1113 GAAGAC BsoBI 494 CTCGGG C^[CT]CG[GA]G 965 CTCGAG BstYI 506 GGATCC [GA]^GATC[CT] 797 GGATCT Cac8I 666 GCATGC GC[ACGT]^[ACGT]GC 1101 GCGAGC Eam1105I 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1306 GACAAGATGTC KpnI 986 GGTACC GGTAC^C 1354 GGTACC MfeI 224 CAATTG C^AATTG 815 CAATTG MflI 506 GGATCC [GA]^GATC[CT] 797 GGATCT MseI 851 TTAA T^TAA 1145 TTAA MslI 222 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 552 CATGGCTGTG MunI 224 CAATTG C^AATTG 815 CAATTG NciI 30 CCGGG CC^[GC]GG 996 CCGGG PleI 68 GAGTC GAGTC(4/5) 1126 GAGTC PpuMI 701 AGGACCT [GA]G^G[AT]CC[CT] 899 GGGACCT SexAI 935 ACCAGGT A^CC[AT]GGT 1229 ACCTGGT TaqI 966 TCGA T^CGA 1260 TCGA TfiI 81 GATTC G^A[AT]TC 1134 GATTC Tru9I 851 TTAA T^TAA 1145 TTAA Tsp45I 559 GTGAC ^GT[GC]AC 1128 GTCAC TthHB8I 966 TCGA T^CGA 1260 TCGA XcmI 115 CCAGATGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 563 CCACACATGCCCTGG XhoII 506 GGATCC [GA]^GATC[CT] 797 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI