(Circular) (Six-base) MAPSORT of: pFN21AE4448.seq Check: 366 from: 1 to: 6094 pFN21AE4448 6094 bp With 182 enzymes: SgfI * March 28, 2012 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 3437 1937 Size: 1221 279 4594 Fragments arranged by size: 4594 1221 279 Acc65I G'GTAC_C Cuts at: 2991 3359 3415 2991 Size: 368 56 5670 Fragments arranged by size: 5670 368 56 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 4157 5541 5809 1632 Size: 746 1779 1384 268 1917 Fragments arranged by size: 1917 1779 1384 746 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 2537 3330 4993 5970 1203 Size: 1334 793 1663 977 1327 Fragments arranged by size: 1663 1334 1327 977 793 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 2428 3316 4823 2428 Size: 888 1507 3699 Fragments arranged by size: 3699 1507 888 AleI CACnn'nnGTG Cuts at: 1166 2231 1166 Size: 1065 5029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 2056 3025 3837 5409 2056 Size: 969 812 1572 2741 Fragments arranged by size: 2741 1572 969 812 ApaI G_GGCC'C Cuts at: 2262 2262 Size: 6094 ApaLI G'TGCA_C Cuts at: 2699 4151 5307 2699 Size: 1452 1156 3486 Fragments arranged by size: 3486 1452 1156 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2499 2970 3419 1870 Size: 73 556 471 449 4545 Fragments arranged by size: 4545 556 471 449 73 BamHI G'GATC_C Cuts at: 2511 3424 3791 2511 Size: 913 367 4814 Fragments arranged by size: 4814 913 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2991 3359 3415 4870 618 Size: 325 217 627 1204 368 56 1455 1842 Fragments arranged by size: 1842 1455 1204 627 368 325 217 56 BanII G_rGCy'C Cuts at: 1391 2262 2349 2665 2891 3413 1391 Size: 871 87 316 226 522 4072 Fragments arranged by size: 4072 871 522 316 226 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2921 3125 928 Size: 971 1022 204 3897 Fragments arranged by size: 3897 1022 971 204 BbvCI CC'TCA_GC Cuts at: 2015 2015 Size: 6094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2961 2995 4285 4319 1903 Size: 34 1024 34 1290 34 3678 Fragments arranged by size: 3678 1290 1024 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BfrBI ATG'CAT Cuts at: 2671 2671 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3206 3299 Size: 107 122 71 631 605 337 1197 93 Cuts at: 3299 4705 136 Size: 1406 1525 Fragments arranged by size: 1525 1406 1197 631 605 337 122 107 93 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 2137 2262 2506 2703 4155 5311 1790 Size: 347 125 244 197 1452 1156 2573 Fragments arranged by size: 2573 1452 1156 347 244 197 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3825 4783 442 Size: 416 53 83 399 24 2408 958 1753 Fragments arranged by size: 2408 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 2679 3165 4754 1239 Size: 852 588 486 1589 2579 Fragments arranged by size: 2579 1589 852 588 486 Bpu10I CC'TnA_GC Cuts at: 2015 2890 3102 2015 Size: 875 212 5007 Fragments arranged by size: 5007 875 212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4219 5084 5382 4219 Size: 865 298 4931 Fragments arranged by size: 4931 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4757 882 Size: 617 3258 2219 Fragments arranged by size: 3258 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3901 4283 4918 Size: 53 83 186 936 507 1861 382 635 Cuts at: 4918 275 Size: 1451 Fragments arranged by size: 1861 1451 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4527 4925 5199 5346 5757 4527 Size: 398 274 147 411 4864 Fragments arranged by size: 4864 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2618 2648 1178 Size: 30 165 30 1215 30 4624 Fragments arranged by size: 4624 1215 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2484 2487 2709 2795 3023 1186 Size: 1298 3 222 86 228 4257 Fragments arranged by size: 4257 1298 228 222 86 3 BseYI C'CCAG_C Cuts at: 2093 2269 2919 5297 5793 2093 Size: 176 650 2378 496 2394 Fragments arranged by size: 2394 2378 650 496 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 1163 Size: 573 600 4921 Fragments arranged by size: 4921 600 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3544 4305 5333 1540 Size: 275 186 1543 761 1028 2301 Fragments arranged by size: 2301 1543 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 4155 4240 5311 2703 Size: 1452 85 1071 3486 Fragments arranged by size: 3486 1452 1071 85 BsmI GAATG_Cn' Cuts at: 2673 3605 3698 2673 Size: 932 93 5069 Fragments arranged by size: 5069 932 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2262 2349 2506 2665 2703 2891 Size: 399 347 125 87 157 159 38 188 Cuts at: 2891 3413 4155 4240 5311 1391 Size: 522 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 399 347 188 159 157 125 87 85 38 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2916 3449 844 Size: 2072 533 3489 Fragments arranged by size: 3489 2072 533 BsrBI CCG'CTC Cuts at: 1394 1823 3981 1394 Size: 429 2158 3507 Fragments arranged by size: 3507 2158 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 4738 4925 1700 Size: 110 605 2323 187 2869 Fragments arranged by size: 2869 2323 605 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6094 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 3302 3302 Size: 6094 BstYI r'GATC_y Cuts at: 1972 2511 2802 3424 3791 4176 4193 5645 5731 Size: 539 291 622 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 622 539 385 367 346 291 86 17 12 BstZ17I GTA'TAC Cuts at: 3159 3159 Size: 6094 BtgI C'CryG_G Cuts at: 513 1065 2004 3126 513 Size: 552 939 1122 3481 Fragments arranged by size: 3481 1122 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 2117 3610 4404 4424 2117 Size: 1493 794 20 3787 Fragments arranged by size: 3787 1493 794 20 ClaI AT'CG_AT Cuts at: 1554 3265 1554 Size: 1711 4383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2687 3541 4430 Size: 54 1425 50 275 18 857 854 889 Cuts at: 4430 6059 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 857 854 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 4024 1353 Size: 1413 1258 3423 Fragments arranged by size: 3423 1413 1258 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2378 2679 3165 4157 4754 5541 Size: 393 459 287 301 486 992 597 787 Cuts at: 5541 5809 1239 Size: 268 1524 Fragments arranged by size: 1524 992 787 597 486 459 393 301 287 268 EcoO109I rG'GnC_Cy Cuts at: 2259 2398 2707 2905 2259 Size: 139 309 198 5448 Fragments arranged by size: 5448 309 198 139 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 6094 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2015 5241 1678 Size: 337 3226 2531 Fragments arranged by size: 3226 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 2639 2671 3258 Size: 32 135 32 842 32 193 32 587 Cuts at: 3258 3265 3290 3297 3302 3334 4735 4767 4809 Size: 7 25 7 5 32 1401 32 42 Cuts at: 4809 4841 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 842 587 193 135 42 32 32 32 32 32 32 32 25 7 7 5 HincII GTy'rAC Cuts at: 669 1657 1938 3438 3684 669 Size: 988 281 1500 246 3079 Fragments arranged by size: 3079 1500 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KpnI G_GTAC'C Cuts at: 2995 3363 3419 2995 Size: 368 56 5670 Fragments arranged by size: 5670 368 56 MfeI C'AATT_G Cuts at: 2229 2820 3693 2229 Size: 591 873 4630 Fragments arranged by size: 4630 873 591 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 5208 5392 1305 Size: 1452 2451 184 2007 Fragments arranged by size: 2451 2007 1452 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4272 Fragments arranged by size: 4272 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2561 4052 4411 4570 518 Size: 648 1065 330 1491 359 159 2042 Fragments arranged by size: 2042 1491 1065 648 359 330 159 MspA1I CmG'CkG Cuts at: 1606 3510 4187 5335 5580 1606 Size: 1904 677 1148 245 2120 Fragments arranged by size: 2120 1904 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3126 513 Size: 552 939 1122 3481 Fragments arranged by size: 3481 1122 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NsiI A_TGCA'T Cuts at: 2673 2673 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 2576 2675 3334 3452 4997 1132 Size: 97 1347 99 659 118 1545 2229 Fragments arranged by size: 2229 1545 1347 659 118 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6094 PciI A'CATG_T Cuts at: 3330 4993 3330 Size: 1663 4431 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 1108 Size: 1449 4645 PfoI T'CCnGG_A Cuts at: 3181 3181 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 2905 2707 Size: 198 5896 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 2258 2258 Size: 6094 PstI C_TGCA'G Cuts at: 830 3446 830 Size: 2616 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SexAI A'CCwGG_T Cuts at: 2940 3234 2940 Size: 294 5800 SfcI C'TryA_G Cuts at: 826 1045 3442 4577 5258 5449 826 Size: 219 2397 1135 681 191 1471 Fragments arranged by size: 2397 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2970 4198 5099 5361 820 Size: 197 926 1027 1228 901 262 1553 Fragments arranged by size: 1553 1228 1027 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 2293 152 Size: 2141 3953 SphI G_CATG'C Cuts at: 1132 2675 3452 1132 Size: 1543 777 3774 Fragments arranged by size: 3774 1543 777 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2453 1517 Size: 936 5158 StyI C'CwwG_G Cuts at: 513 1065 2004 2198 2584 2716 3126 3213 3533 Size: 552 939 194 386 132 410 87 320 Cuts at: 3533 513 Size: 3074 Fragments arranged by size: 3074 939 552 410 386 320 194 132 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 4121 4138 4291 4476 4921 325 Size: 1255 703 1838 17 153 185 445 1498 Fragments arranged by size: 1838 1498 1255 703 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4340 6052 96 Size: 274 80 33 51 1443 2363 1712 138 Fragments arranged by size: 2363 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 6054 6086 2496 Size: 32 3526 32 2504 Fragments arranged by size: 3526 2504 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2127 2575 2127 Size: 448 5646 XhoI C'TCGA_G Cuts at: 1943 2970 1943 Size: 1027 5067 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI