(Linear) (Six-base) MAPSORT of: KIEE4448.seq Check: 3530 from: 1 to: 1389 KIEE4448.orf With 182 enzymes: SgfI * March 22, 2012 18:03 .. AccI GT'mk_AC Cuts at: 0 1153 1389 Size: 1153 236 Acc65I G'GTAC_C Cuts at: 0 986 1354 1389 Size: 986 368 35 Fragments arranged by size: 986 368 35 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 1389 Size: 373 1016 AflIII A'CryG_T Cuts at: 0 532 1325 1389 Size: 532 793 64 Fragments arranged by size: 793 532 64 AhdI GACnn_n'nnGTC Cuts at: 0 423 1311 1389 Size: 423 888 78 Fragments arranged by size: 888 423 78 AleI CACnn'nnGTG Cuts at: 0 226 1389 Size: 226 1163 AlwNI CAG_nnn'CTG Cuts at: 0 51 1020 1389 Size: 51 969 369 Fragments arranged by size: 969 369 51 ApaI G_GGCC'C Cuts at: 0 257 1389 Size: 257 1132 ApaLI G'TGCA_C Cuts at: 0 694 1389 Size: 694 695 AvaI C'yCGr_G Cuts at: 0 494 965 1389 Size: 494 471 424 Fragments arranged by size: 494 471 424 BamHI G'GATC_C Cuts at: 0 506 1389 Size: 506 883 BanI G'GyrC_C Cuts at: 0 986 1354 1389 Size: 986 368 35 Fragments arranged by size: 986 368 35 BanII G_rGCy'C Cuts at: 0 257 344 660 886 1389 Size: 257 87 316 226 503 Fragments arranged by size: 503 316 257 226 87 BbsI GAAGACnn'nnnn_ Cuts at: 0 916 1120 1389 Size: 916 204 269 Fragments arranged by size: 916 269 204 BbvCI CC'TCA_GC Cuts at: 0 10 1389 Size: 10 1379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 956 990 1389 Size: 956 34 399 Fragments arranged by size: 956 399 34 BfrBI ATG'CAT Cuts at: 0 666 1389 Size: 666 723 BglI GCCn_nnn'nGGC Cuts at: 0 1201 1294 1389 Size: 1201 93 95 Fragments arranged by size: 1201 95 93 Bme1580I G_kGCm'C Cuts at: 0 132 257 501 698 1389 Size: 132 125 244 197 691 Fragments arranged by size: 691 244 197 132 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 674 1160 1389 Size: 86 588 486 229 Fragments arranged by size: 588 486 229 86 Bpu10I CC'TnA_GC Cuts at: 0 10 885 1097 1389 Size: 10 875 212 292 Fragments arranged by size: 875 292 212 10 BsaHI Gr'CG_yC Cuts at: 0 35 1389 Size: 35 1354 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 613 643 1389 Size: 613 30 746 Fragments arranged by size: 746 613 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 479 482 704 790 1018 1389 Size: 479 3 222 86 228 371 Fragments arranged by size: 479 371 228 222 86 3 BseYI C'CCAG_C Cuts at: 0 88 264 914 1389 Size: 88 176 650 475 Fragments arranged by size: 650 475 176 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1389 Size: 331 1058 BsiHKAI G_wGCw'C Cuts at: 0 698 1389 Size: 698 691 BsmI GAATG_Cn' Cuts at: 0 668 1389 Size: 668 721 Bsp1286I G_dGCh'C Cuts at: 0 132 257 344 501 660 698 886 1389 Size: 132 125 87 157 159 38 188 503 Fragments arranged by size: 503 188 159 157 132 125 87 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 911 1389 Size: 911 478 BsrFI r'CCGG_y Cuts at: 0 410 1389 Size: 410 979 BstXI CCAn_nnnn'nTGG Cuts at: 0 1297 1389 Size: 1297 92 BstYI r'GATC_y Cuts at: 0 506 797 1389 Size: 506 291 592 Fragments arranged by size: 592 506 291 BstZ17I GTA'TAC Cuts at: 0 1154 1389 Size: 1154 235 BtgI C'CryG_G Cuts at: 0 1121 1389 Size: 1121 268 BtsI GCAGTG_nn' Cuts at: 0 112 1389 Size: 112 1277 ClaI AT'CG_AT Cuts at: 0 1260 1389 Size: 1260 129 EaeI y'GGCC_r Cuts at: 0 682 1389 Size: 682 707 EarI CTCTTCn'nnn_ Cuts at: 0 761 1389 Size: 761 628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 373 674 1160 1389 Size: 86 287 301 486 229 Fragments arranged by size: 486 301 287 229 86 EcoO109I rG'GnC_Cy Cuts at: 0 254 393 702 900 1389 Size: 254 139 309 198 489 Fragments arranged by size: 489 309 254 198 139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 865 1389 Size: 865 524 HaeII r_GCGC'y Cuts at: 0 10 1389 Size: 10 1379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 409 441 634 666 1253 1260 1285 1292 Size: 409 32 193 32 587 7 25 7 Cuts at: 1292 1297 1329 1389 Size: 5 32 60 Fragments arranged by size: 587 409 193 60 32 32 32 25 7 7 5 KpnI G_GTAC'C Cuts at: 0 990 1358 1389 Size: 990 368 31 Fragments arranged by size: 990 368 31 MfeI C'AATT_G Cuts at: 0 224 815 1389 Size: 224 591 574 Fragments arranged by size: 591 574 224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 752 1389 Size: 752 637 MslI CAynn'nnrTG Cuts at: 0 226 556 1389 Size: 226 330 833 Fragments arranged by size: 833 330 226 NcoI C'CATG_G Cuts at: 0 1121 1389 Size: 1121 268 NsiI A_TGCA'T Cuts at: 0 668 1389 Size: 668 721 NspI r_CATG'y Cuts at: 0 571 670 1329 1389 Size: 571 99 659 60 Fragments arranged by size: 659 571 99 60 PciI A'CATG_T Cuts at: 0 1325 1389 Size: 1325 64 PflMI CCAn_nnn'nTGG Cuts at: 0 552 1389 Size: 552 837 PfoI T'CCnGG_A Cuts at: 0 1176 1389 Size: 1176 213 PpuMI rG'GwC_Cy Cuts at: 0 702 900 1389 Size: 702 198 489 Fragments arranged by size: 702 489 198 PspOMI G'GGCC_C Cuts at: 0 253 1389 Size: 253 1136 SexAI A'CCwGG_T Cuts at: 0 935 1229 1389 Size: 935 294 160 Fragments arranged by size: 935 294 160 SmlI C'TyrA_G Cuts at: 0 965 1389 Size: 965 424 SpeI A'CTAG_T Cuts at: 0 288 1389 Size: 288 1101 SphI G_CATG'C Cuts at: 0 670 1389 Size: 670 719 StuI AGG'CCT Cuts at: 0 448 1389 Size: 448 941 StyI C'CwwG_G Cuts at: 0 193 579 711 1121 1208 1389 Size: 193 386 132 410 87 181 Fragments arranged by size: 410 386 193 181 132 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 278 1389 Size: 278 1111 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 491 523 1389 Size: 491 32 866 Fragments arranged by size: 866 491 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 122 570 1389 Size: 122 448 819 Fragments arranged by size: 819 448 122 XhoI C'TCGA_G Cuts at: 0 965 1389 Size: 965 424 Enzymes that do cut: AccI Acc65I AcuI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstXI BstYI BstZ17I BtgI BtsI ClaI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MfeI MmeI MslI NcoI NsiI NspI PciI PflMI PfoI PpuMI PspOMI SexAI SmlI SpeI SphI StuI StyI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI TatI Tth111I XbaI XmaI XmnI ZraI