################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BlnI BpmI BpuAI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HaeIII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I TspRI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 614 GAATTT [GA]^AATT[CT] AcyI 314 GGCGTC G[GA]^CG[CT]C AfaI 381 GTAC GT^AC AhdI 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 614 GAATTT [GA]^AATT[CT] AspEI 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 65 GTGCTC G[AT]GC[AT]^C BciVI 817 GGATAC GTATCC(6/5) BcnI 206 CCCGG CC^[GC]GG BlpI 372 GCTGAGC GC^T[ACGT]AGC BmyI 65 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 372 GCTGAGC GC^T[ACGT]AGC BsaAI 242 CACGTA [CT]AC^GT[GA] BsaHI 314 GGCGTC G[GA]^CG[CT]C BsiHKAI 65 GTGCTC G[AT]GC[AT]^C Bsp1286I 65 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 380 TGTACA T^GTACA BspMI 248 GCAGGT ACCTGC(4/8) BsrDI 429 CATTGC GCAATG(2/0) BsrGI 380 TGTACA T^GTACA BstEII 788 GGTCACC G^GT[ACGT]ACC BstF5I 745 GGATG GGATG(2/0) BstPI 788 GGTCACC G^GT[ACGT]ACC BstXI 536 CCAATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 372 GCTGAGC GC^T[ACGT]AGC DraII 151 AGGTCCC [GA]G^G[ACGT]CC[CT] Eam1105I 454 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 151 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 788 GGTCACC G^GT[ACGT]ACC FokI 745 GGATG GGATG(9/13) HapII 207 CCGG C^CGG HgaI 315 GCGTC GACGC(5/10) Hin1I 314 GGCGTC G[GA]^CG[CT]C HpaII 207 CCGG C^CGG MspA1I 9 CAGCGG C[AC]G^C[GT]G MspI 207 CCGG C^CGG NciI 206 CCCGG CC^[GC]GG PpuMI 151 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 381 GTAC GT^AC SfaNI 511 GATGC GCATC(5/9) SspBI 380 TGTACA T^GTACA Tsp509I 615 AATT ^AATT XbaI 172 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 272 GTAGAC GT^[AC][GT]AC 303 GTCTAC AvaII 152 GGTCC G^G[AT]CC 424 GGTCC BbvI 641 GCAGC GCAGC(8/12) 754 GCTGC BglII 396 AGATCT A^GATCT 835 AGATCT BsaJI 116 CCAAGG C^C[ACGT][ACGT]GG 515 CCAAGG BsrBI 225 CCGCTC CCGCTC(-3/-3) 31 GAGCGG Cfr13I 152 GGTCC G^G[ACGT]CC 424 GGTCC DdeI 373 CTGAG C^T[ACGT]AG 400 CTAAG EcoT14I 116 CCAAGG C^C[AT][AT]GG 515 CCAAGG FauI 224 CCCGC CCCGC(4/6) 33 GCGGG HphI 790 TCACC GGTGA(8/7) 850 TCACC MaeIII 210 GTCAC ^GT[ACGT]AC 789 GTCAC MboII 121 GAAGA GAAGA(8/7) 182 GAAGA NlaIV 152 GGTCCC GG[ACGT]^[ACGT]CC 424 GGTCCC Sau96I 152 GGTCC G^G[ACGT]CC 424 GGTCC SfcI 705 CTATAG C^T[GA][CT]AG 804 CTATAG StyI 116 CCAAGG C^C[AT][AT]GG 515 CCAAGG TaqI 261 TCGA T^CGA 840 TCGA TseI 641 GCAGC G^C[AT]GC 754 GCTGC Tsp45I 210 GTCAC ^GT[GC]AC 789 GTCAC TthHB8I 261 TCGA T^CGA 840 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI AlwI BfaI BsaBI BsaI BseRI BsiYI BslI BsmAI BsmFI BssKI BstNI BstYI Cac8I DpnII EcoRII Fnu4HI HinfI ItaI MaeI MaeII MamI MboI MflI MnlI MvaI MwoI NdeII NlaIII Sau3AI ScrFI TfiI XhoII