Restriction map for
pFN21AB3484
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 159 CCGC CCGC(-3/-1)
AcsI 23 AAATTC [GA]^AATT[CT]
AfaI 68 GTAC GT^AC
AhdI 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 23 AAATTC [GA]^AATT[CT]
Asp700I 19 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 35 GAGCTC G[AT]GC[AT]^C
AvaII 177 GGACC G^G[AT]CC
BanII 35 GAGCTC G[GA]GC[CT]^C
BbsI 210 GAAGAC GAAGAC(2/6)
BbvI 400 GCAGC GCAGC(8/12)
BcnI 360 CCCGG CC^[GC]GG
BmyI 35 GAGCTC G[AGT]GC[ACT]^C
BpuAI 210 GAAGAC GAAGAC(2/6)
BseRI 72 GAGGAG GAGGAG(10/8)
BsiHKAI 35 GAGCTC G[AT]GC[AT]^C
BsiYI 388 CCAACAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 388 CCAACAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 35 GAGCTC G[AGT]GC[ACT]^C
BsrI 404 CCAGT ACTGG(1/-1)
DdeI 101 CTCAG C^T[ACGT]AG
DpnII 586 GATC ^GATC
Eam1105I 366 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 457 GAAGAG CTCTTC(1/4)
Fnu4HI 400 GCAGC GC^[ACGT]GC
HapII 361 CCGG C^CGG
HpaII 361 CCGG C^CGG
ItaI 400 GCAGC GC^[ACGT]GC
Ksp632I 457 GAAGAG CTCTTC(1/4)
MaeIII 364 GTGAC ^GT[ACGT]AC
MboI 586 GATC ^GATC
MnlI 72 GAGG CCTC(7/6)
MslI 123 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 361 CCGG C^CGG
NciI 360 CCCGG CC^[GC]GG
NdeII 586 GATC ^GATC
RsaI 68 GTAC GT^AC
SacI 35 GAGCTC GAGCT^C
Sau3AI 586 GATC ^GATC
SmlI 470 CTCAAG C^T[CT][GA]AG
TseI 400 GCAGC G^C[AT]GC
Tsp45I 364 GTGAC ^GT[GC]AC
Tsp509I 24 AATT ^AATT
TspRI 405 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 122 CCATCACTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 19 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AluI 36 AGCT AG^CT
377 AGCT
BciVI 412 GGATAC GTATCC(6/5)
482 GTATCC
BsaJI 81 CCTGGG C^C[ACGT][ACGT]GG
486 CCTGGG
BsrDI 335 GCAATG GCAATG(2/0)
521 GCAATG
BstF5I 105 GGATG GGATG(2/0)
135 GGATG
FokI 105 GGATG GGATG(9/13)
135 GGATG
MseI 217 TTAA T^TAA
419 TTAA
Tru9I 217 TTAA T^TAA
419 TTAA
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BsmAI BssKI BstNI Cfr13I EcoRII
HaeIII HinfI HphI MboII MvaI
NlaIII PleI Sau96I ScrFI SfaNI
TfiI