################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsiWI BsmI Bsp1407I BspDI BspHI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 281 TCCGGA T^CCGGA ApaI 788 GGGCCC GGGCC^C AspI 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1971 GCTGAGG CCTCAGC(-5/-2) BlpI 510 GCTGAGC GC^T[ACGT]AGC Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC BsaBI 1326 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 281 TCCGGA [AT]^CCGG[AT] BseAI 281 TCCGGA T^CCGGA BsiEI 987 CGGCCG CG[GA][CT]^CG BsmBI 1036 GAGACG CGTCTC(1/5) BspEI 281 TCCGGA T^CCGGA BspLU11I 1862 ACATGT A^CATGT BsrFI 737 GCCGGT [GA]^CCGG[CT] BssSI 1147 CACGAG CACGAG(-5/-1) BstXI 1416 CCATGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1745 AGATCC [GA]^GATC[CT] CelII 510 GCTGAGC GC^T[ACGT]AGC Cfr10I 737 GCCGGT [GA]^CCGG[CT] EagI 987 CGGCCG C^GGCCG EarI 1645 CTCTTC CTCTTC(1/4) EclXI 987 CGGCCG C^GGCCG Eco52I 987 CGGCCG C^GGCCG Ksp632I 1645 CTCTTC CTCTTC(1/4) KspI 1249 CCGCGG CCGC^GG MamI 1326 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1745 AGATCC [GA]^GATC[CT] MroI 281 TCCGGA T^CCGGA PflFI 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 1249 CCGCGG CCGC^GG SfiI 1338 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1595 CCCGGG CCC^GGG TfiI 936 GATTC G^A[AT]TC Tth111I 829 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1745 AGATCC [GA]^GATC[CT] XmaCI 1595 CCCGGG C^CCGGG XmaI 1595 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 419 GGTACC G^GTACC 1832 GGTACC AlwI 1746 GATCC GGATC(4/5) 362 GGATC Asp718I 419 GGTACC G^GTACC 1832 GGTACC BbeI 1156 GGCGCC GGCGC^C 1506 GGCGCC BcgI 557 CGACCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1719 CGAGGAGTATGC BfaI 586 CTAG C^TAG 883 CTAG BglI 1330 GCCATCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1339 GCCATGGTGGC BsrBI 427 GAGCGG CCGCTC(-3/-3) 1489 GAGCGG BstAPI 13 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1098 GCACGGGCTGC BstEII 216 GGTCACC G^GT[ACGT]ACC 648 GGTCACC BstPI 216 GGTCACC G^GT[ACGT]ACC 648 GGTCACC EcoO65I 216 GGTCACC G^GT[ACGT]ACC 648 GGTCACC KasI 1156 GGCGCC G^GCGCC 1506 GGCGCC KpnI 419 GGTACC GGTAC^C 1832 GGTACC MaeI 586 CTAG C^TAG 883 CTAG MslI 44 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1857 CATGGACATG NarI 1156 GGCGCC GG^CGCC 1506 GGCGCC NcoI 1340 CCATGG C^CATGG 1856 CCATGG PflMI 864 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1160 CCAGTGCCTGG ScaI 1073 AGTACT AGT^ACT 1823 AGTACT SexAI 146 ACCAGGT A^CC[AT]GGT 1025 ACCAGGT SfoI 1156 GGCGCC GGC^GCC 1506 GGCGCC StuI 725 AGGCCT AGG^CCT 1641 AGGCCT TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^ 1161 CAGTG Van91I 864 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1160 CCAGTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TthHB8I XcmI