(Linear) (Six-base) MAPSORT of: KIEE4562.seq Check: 3825 from: 1 to: 2067 KIEE4562.orf With 182 enzymes: SgfI * March 22, 2012 18:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1077 1795 2067 Size: 1077 718 272 Fragments arranged by size: 1077 718 272 Acc65I G'GTAC_C Cuts at: 0 419 1832 2067 Size: 419 1413 235 Fragments arranged by size: 1413 419 235 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 967 2067 Size: 703 264 1100 Fragments arranged by size: 1100 703 264 AflIII A'CryG_T Cuts at: 0 173 350 1862 2067 Size: 173 177 1512 205 Fragments arranged by size: 1512 205 177 173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 66 944 1606 2044 2067 Size: 66 878 662 438 23 Fragments arranged by size: 878 662 438 66 23 AlwNI CAG_nnn'CTG Cuts at: 0 1062 1166 1521 1689 1928 2067 Size: 1062 104 355 168 239 139 Fragments arranged by size: 1062 355 239 168 139 104 ApaI G_GGCC'C Cuts at: 0 792 2067 Size: 792 1275 AvaI C'yCGr_G Cuts at: 0 406 598 1209 1595 2050 2067 Size: 406 192 611 386 455 17 Fragments arranged by size: 611 455 406 386 192 17 BanI G'GyrC_C Cuts at: 0 154 419 1156 1506 1832 2067 Size: 154 265 737 350 326 235 Fragments arranged by size: 737 350 326 265 235 154 BanII G_rGCy'C Cuts at: 0 119 596 792 1127 1604 2051 2067 Size: 119 477 196 335 477 447 16 Fragments arranged by size: 477 477 447 335 196 119 16 BbeI G_GCGC'C Cuts at: 0 1160 1510 2067 Size: 1160 350 557 Fragments arranged by size: 1160 557 350 BbvCI CC'TCA_GC Cuts at: 0 1972 2067 Size: 1972 95 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 546 580 1708 1742 2067 Size: 546 34 1128 34 325 Fragments arranged by size: 1128 546 325 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1336 1345 2067 Size: 1336 9 722 Fragments arranged by size: 1336 722 9 BlpI GC'TnA_GC Cuts at: 0 511 2067 Size: 511 1556 Bme1580I G_kGCm'C Cuts at: 0 90 792 1271 1595 2067 Size: 90 702 479 324 472 Fragments arranged by size: 702 479 472 324 90 BmgBI CAC'GTC Cuts at: 0 174 327 351 1179 2067 Size: 174 153 24 828 888 Fragments arranged by size: 888 828 174 153 24 BmrI ACTGGGnnnn_n' Cuts at: 0 1539 1815 2067 Size: 1539 276 252 Fragments arranged by size: 1539 276 252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 445 562 640 859 961 1543 1627 Size: 358 87 117 78 219 102 582 84 Cuts at: 1627 2067 Size: 440 Fragments arranged by size: 582 440 358 219 117 102 87 84 78 Bpu10I CC'TnA_GC Cuts at: 0 1972 2017 2067 Size: 1972 45 50 Fragments arranged by size: 1972 50 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 640 751 1051 2067 Size: 178 462 111 300 1016 Fragments arranged by size: 1016 462 300 178 111 BsaI GGTCTCn'nnnn_ Cuts at: 0 697 1399 1932 2067 Size: 697 702 533 135 Fragments arranged by size: 702 697 533 135 BsaAI yAC'GTr Cuts at: 0 696 1431 1485 2067 Size: 696 735 54 582 Fragments arranged by size: 735 696 582 54 BsaBI GATnn'nnATC Cuts at: 0 1330 2067 Size: 1330 737 BsaHI Gr'CG_yC Cuts at: 0 1157 1507 1817 2006 2067 Size: 1157 350 310 189 61 Fragments arranged by size: 1157 350 310 189 61 BsaWI w'CCGG_w Cuts at: 0 281 2067 Size: 281 1786 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 337 367 1495 1525 2067 Size: 337 30 1128 30 542 Fragments arranged by size: 1128 542 337 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 736 763 1048 1165 1735 2067 Size: 115 621 27 285 117 570 332 Fragments arranged by size: 621 570 332 285 117 115 27 BseYI C'CCAG_C Cuts at: 0 89 254 441 807 1107 2067 Size: 89 165 187 366 300 960 Fragments arranged by size: 960 366 300 187 165 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 1105 1675 2067 Size: 41 1064 570 392 Fragments arranged by size: 1064 570 392 41 BsiEI CG_ry'CG Cuts at: 0 990 2067 Size: 990 1077 BsiHKAI G_wGCw'C Cuts at: 0 55 119 190 1127 1604 2067 Size: 55 64 71 937 477 463 Fragments arranged by size: 937 477 463 71 64 55 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1030 2067 Size: 1030 1037 Bsp1286I G_dGCh'C Cuts at: 0 55 90 119 190 596 792 1127 1271 Size: 55 35 29 71 406 196 335 144 Cuts at: 1271 1595 1604 2051 2067 Size: 324 9 447 16 Fragments arranged by size: 447 406 335 324 196 144 71 55 35 29 16 9 BspEI T'CCGG_A Cuts at: 0 281 2067 Size: 281 1786 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 77 1070 1077 1661 1795 2022 2049 2067 Size: 77 993 7 584 134 227 27 18 Fragments arranged by size: 993 584 227 134 77 27 18 7 BsrBI CCG'CTC Cuts at: 0 429 1491 2067 Size: 429 1062 576 Fragments arranged by size: 1062 576 429 BsrFI r'CCGG_y Cuts at: 0 737 2067 Size: 737 1330 BssSI C'ACGA_G Cuts at: 0 1147 2067 Size: 1147 920 BstAPI GCAn_nnn'nTGC Cuts at: 0 19 1104 2067 Size: 19 1085 963 Fragments arranged by size: 1085 963 19 BstEII G'GTnAC_C Cuts at: 0 216 648 2067 Size: 216 432 1419 Fragments arranged by size: 1419 432 216 BstXI CCAn_nnnn'nTGG Cuts at: 0 1423 2067 Size: 1423 644 BstYI r'GATC_y Cuts at: 0 1745 2067 Size: 1745 322 BtgI C'CryG_G Cuts at: 0 869 1249 1340 1856 2067 Size: 869 380 91 516 211 Fragments arranged by size: 869 516 380 211 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1317 2067 Size: 1317 750 EaeI y'GGCC_r Cuts at: 0 203 851 872 987 1337 1346 1425 1760 Size: 203 648 21 115 350 9 79 335 Cuts at: 1760 1794 2067 Size: 34 273 Fragments arranged by size: 648 350 335 273 203 115 79 34 21 9 EagI C'GGCC_G Cuts at: 0 987 2067 Size: 987 1080 EarI CTCTTCn'nnn_ Cuts at: 0 1651 2067 Size: 1651 416 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 49 1471 2067 Size: 49 1422 596 Fragments arranged by size: 1422 596 49 EcoICRI GAG'CTC Cuts at: 0 117 1125 1602 2067 Size: 117 1008 477 465 Fragments arranged by size: 1008 477 465 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 445 562 640 703 859 961 967 Size: 358 87 117 78 63 156 102 6 Cuts at: 967 1543 1627 2067 Size: 576 84 440 Fragments arranged by size: 576 440 358 156 117 102 87 84 78 63 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 1066 1306 1672 2020 2067 Size: 547 519 240 366 348 47 Fragments arranged by size: 547 519 366 348 240 47 EcoO109I rG'GnC_Cy Cuts at: 0 78 972 1478 1686 1755 2067 Size: 78 894 506 208 69 312 Fragments arranged by size: 894 506 312 208 78 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1092 2067 Size: 1092 975 HaeII r_GCGC'y Cuts at: 0 202 969 1160 1510 1589 1967 1980 2067 Size: 202 767 191 350 79 378 13 87 Fragments arranged by size: 767 378 350 202 191 87 79 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 60 160 192 1192 1224 1363 1395 Size: 28 32 100 32 1000 32 139 32 Cuts at: 1395 1495 1527 2067 Size: 100 32 540 Fragments arranged by size: 1000 540 139 100 100 32 32 32 32 32 28 KasI G'GCGC_C Cuts at: 0 1156 1506 2067 Size: 1156 350 561 Fragments arranged by size: 1156 561 350 KpnI G_GTAC'C Cuts at: 0 423 1836 2067 Size: 423 1413 231 Fragments arranged by size: 1413 423 231 MscI TGG'CCA Cuts at: 0 853 874 1348 1427 2067 Size: 853 21 474 79 640 Fragments arranged by size: 853 640 474 79 21 MslI CAynn'nnrTG Cuts at: 0 48 1861 2067 Size: 48 1813 206 Fragments arranged by size: 1813 206 48 MspA1I CmG'CkG Cuts at: 0 1251 1475 1551 2067 Size: 1251 224 76 516 Fragments arranged by size: 1251 516 224 76 NarI GG'CG_CC Cuts at: 0 1157 1507 2067 Size: 1157 350 560 Fragments arranged by size: 1157 560 350 NcoI C'CATG_G Cuts at: 0 1340 1856 2067 Size: 1340 516 211 Fragments arranged by size: 1340 516 211 NspI r_CATG'y Cuts at: 0 262 1011 1782 1866 2067 Size: 262 749 771 84 201 Fragments arranged by size: 771 749 262 201 84 PasI CC'CwG_GG Cuts at: 0 912 2067 Size: 912 1155 PciI A'CATG_T Cuts at: 0 1862 2067 Size: 1862 205 PflMI CCAn_nnn'nTGG Cuts at: 0 870 1166 2067 Size: 870 296 901 Fragments arranged by size: 901 870 296 PfoI T'CCnGG_A Cuts at: 0 374 578 836 1604 2067 Size: 374 204 258 768 463 Fragments arranged by size: 768 463 374 258 204 PmlI CAC'GTG Cuts at: 0 696 1431 1485 2067 Size: 696 735 54 582 Fragments arranged by size: 735 696 582 54 PpuMI rG'GwC_Cy Cuts at: 0 78 1478 1755 2067 Size: 78 1400 277 312 Fragments arranged by size: 1400 312 277 78 PspOMI G'GGCC_C Cuts at: 0 788 2067 Size: 788 1279 PstI C_TGCA'G Cuts at: 0 1292 1391 2033 2067 Size: 1292 99 642 34 Fragments arranged by size: 1292 642 99 34 SacI G_AGCT'C Cuts at: 0 119 1127 1604 2067 Size: 119 1008 477 463 Fragments arranged by size: 1008 477 463 119 SacII CC_GC'GG Cuts at: 0 1252 2067 Size: 1252 815 SanDI GG'GwC_CC Cuts at: 0 1478 1755 2067 Size: 1478 277 312 Fragments arranged by size: 1478 312 277 ScaI AGT'ACT Cuts at: 0 1075 1825 2067 Size: 1075 750 242 Fragments arranged by size: 1075 750 242 SexAI A'CCwGG_T Cuts at: 0 146 1025 2067 Size: 146 879 1042 Fragments arranged by size: 1042 879 146 SfcI C'TryA_G Cuts at: 0 1288 1387 2029 2067 Size: 1288 99 642 38 Fragments arranged by size: 1288 642 99 38 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1345 2067 Size: 1345 722 SfoI GGC'GCC Cuts at: 0 1158 1508 2067 Size: 1158 350 559 Fragments arranged by size: 1158 559 350 SmaI CCC'GGG Cuts at: 0 1597 2067 Size: 1597 470 SmlI C'TyrA_G Cuts at: 0 193 655 766 1066 2067 Size: 193 462 111 300 1001 Fragments arranged by size: 1001 462 300 193 111 StuI AGG'CCT Cuts at: 0 727 1643 2067 Size: 727 916 424 Fragments arranged by size: 916 727 424 StyI C'CwwG_G Cuts at: 0 272 1340 1673 1749 1856 2067 Size: 272 1068 333 76 107 211 Fragments arranged by size: 1068 333 272 211 107 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 172 1521 2067 Size: 172 1349 546 Fragments arranged by size: 1349 546 172 TatI w'GTAC_w Cuts at: 0 1073 1823 2067 Size: 1073 750 244 Fragments arranged by size: 1073 750 244 Tth111I GACn'n_nGTC Cuts at: 0 832 2067 Size: 832 1235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 714 867 1338 1747 1804 2067 Size: 714 153 471 409 57 263 Fragments arranged by size: 714 471 409 263 153 57 XmaI C'CCGG_G Cuts at: 0 1595 2067 Size: 1595 472 Enzymes that do cut: AarI Acc65I AcuI AflIII AlfI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI SacI SacII SanDI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmtI BplI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI