################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmeI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AgeI 85 ACCGGT A^CCGGT Alw44I 1075 GTGCAC G^TGCAC AlwI 957 GGATC GGATC(4/5) ApaLI 1075 GTGCAC G^TGCAC AseI 289 ATTAAT AT^TAAT AsnI 289 ATTAAT AT^TAAT Asp700I 858 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 946 CACGTG CAC^GTG BfaI 406 CTAG C^TAG BglII 311 AGATCT A^GATCT BpmI 130 CTGGAG CTGGAG(16/14) BsaAI 946 CACGTG [CT]AC^GT[GA] BsaWI 85 ACCGGT [AT]^CCGG[AT] BseRI 641 GAGGAG GAGGAG(10/8) BsmI 611 GCATTC GAATGC(1/-1) BspMI 271 ACCTGC ACCTGC(4/8) BstEII 527 GGTCACC G^GT[ACGT]ACC BstPI 527 GGTCACC G^GT[ACGT]ACC BstXI 65 CCAGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 150 CCATGG C^C[GA][CT]GG EaeI 973 CGGCCA [CT]^GGCC[GA] EcoO65I 527 GGTCACC G^GT[ACGT]ACC HincII 379 GTTGAC GT[CT]^[GA]AC HindII 379 GTTGAC GT[CT]^[GA]AC MaeI 406 CTAG C^TAG NcoI 150 CCATGG C^CATGG PflMI 884 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 85 ACCGGT A^CCGGT PleI 630 GAGTC GAGTC(4/5) PmaCI 946 CACGTG CAC^GTG PmlI 946 CACGTG CAC^GTG PshBI 289 ATTAAT AT^TAAT PstI 994 CTGCAG CTGCA^G SacI 1090 GAGCTC GAGCT^C ScaI 965 AGTACT AGT^ACT SfcI 994 CTGCAG C^T[GA][CT]AG SmlI 961 CTCAAG C^T[CT][GA]AG TfiI 210 GAATC G^A[AT]TC Van91I 884 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 405 TCTAGA T^CTAGA XcmI 755 CCAAGGACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 858 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AciI 165 GCGG CCGC(-3/-1) 373 GCGG AcsI 344 AAATTT [GA]^AATT[CT] 847 GAATTT AlwNI 267 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1021 CAGAAGCTG ApoI 344 AAATTT [GA]^AATT[CT] 847 GAATTT AspHI 1075 GTGCAC G[AT]GC[AT]^C 1090 GAGCTC AvaI 32 CCCGGG C^[CT]CG[GA]G 1040 CCCGGG BbvI 541 GCAGC GCAGC(8/12) 20 GCTGC BsaI 505 GAGACC GGTCTC(1/5) 931 GAGACC BsgI 1005 CTGCAC GTGCAG(16/14) 484 GTGCAG BsiHKAI 1075 GTGCAC G[AT]GC[AT]^C 1090 GAGCTC BsmAI 505 GAGAC GTCTC(1/5) 931 GAGAC BsmFI 44 GGGAC GGGAC(10/14) 632 GTCCC BsoBI 32 CCCGGG C^[CT]CG[GA]G 1040 CCCGGG BspHI 624 TCATGA T^CATGA 1094 TCATGA BstF5I 102 GGATG GGATG(2/0) 729 GGATG BstYI 311 AGATCT [GA]^GATC[CT] 957 GGATCT CfoI 29 GCGC GCG^C 76 GCGC EarI 616 CTCTTC CTCTTC(1/4) 638 GAAGAG Fnu4HI 20 GCTGC GC^[ACGT]GC 541 GCAGC FokI 102 GGATG GGATG(9/13) 729 GGATG HaeII 28 AGCGCC [GA]GCGC^[CT] 75 GGCGCT HhaI 29 GCGC GCG^C 76 GCGC HinP1I 29 GCGC G^CGC 76 GCGC HinfI 210 GAATC G^A[ACGT]TC 630 GAGTC ItaI 20 GCTGC GC^[ACGT]GC 541 GCAGC Ksp632I 616 CTCTTC CTCTTC(1/4) 638 GAAGAG MflI 311 AGATCT [GA]^GATC[CT] 957 GGATCT RcaI 624 TCATGA T^CATGA 1094 TCATGA SmaI 32 CCCGGG CCC^GGG 1040 CCCGGG TseI 20 GCTGC G^C[AT]GC 541 GCAGC Tsp45I 528 GTCAC ^GT[GC]AC 949 GTGAC XhoII 311 AGATCT [GA]^GATC[CT] 957 GGATCT XmaCI 32 CCCGGG C^CCGGG 1040 CCCGGG XmaI 32 CCCGGG C^CCGGG 1040 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AfaI AluI AvaII BanII BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EcoRII EcoT14I HaeIII HapII HpaII HphI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I Tsp509I TspRI TthHB8I