(Circular) (Six-base) MAPSORT of: pFN21AE6309.seq Check: 1515 from: 1 to: 5821 pFN21AE6309 5821 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3006 3884 5268 5536 1632 Size: 1374 878 1384 268 1917 Fragments arranged by size: 1917 1384 1374 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 2090 4652 2090 Size: 2562 3259 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 2277 3031 3564 5136 2277 Size: 754 533 1572 2962 Fragments arranged by size: 2962 1572 754 533 ApaLI G'TGCA_C Cuts at: 3080 3878 5034 3080 Size: 798 1156 3867 Fragments arranged by size: 3867 1156 798 ApoI r'AATT_y Cuts at: 1493 2349 2852 3130 3356 3508 5617 1493 Size: 856 503 278 226 152 2109 1697 Fragments arranged by size: 2109 1697 856 503 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 2295 4375 160 Size: 872 1263 2080 1606 Fragments arranged by size: 2080 1606 1263 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2037 3045 3146 1870 Size: 73 94 1008 101 4545 Fragments arranged by size: 4545 1008 101 94 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3142 4597 618 Size: 325 217 627 1355 1455 1842 Fragments arranged by size: 1842 1455 1355 627 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2335 3099 3140 1391 Size: 654 290 764 41 4072 Fragments arranged by size: 4072 764 654 290 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2316 5816 2316 Size: 3500 2321 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 3084 3882 5038 1790 Size: 1294 798 1156 2573 Fragments arranged by size: 2573 1294 1156 798 BmgBI CAC'GTC Cuts at: 1318 2344 3091 1318 Size: 1026 747 4048 Fragments arranged by size: 4048 1026 747 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2838 3552 4510 Size: 416 53 83 399 24 1421 714 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1421 958 714 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4481 1239 Size: 917 2325 2579 Fragments arranged by size: 2579 2325 917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2951 3946 4811 5109 2951 Size: 995 865 298 3663 Fragments arranged by size: 3663 995 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2504 2930 4484 882 Size: 617 1005 426 1554 2219 Fragments arranged by size: 2219 1554 1005 617 426 BsaAI yAC'GTr Cuts at: 493 1204 2953 3612 493 Size: 711 1749 659 2702 Fragments arranged by size: 2702 1749 711 659 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2090 4254 4652 4926 5073 5484 2090 Size: 2164 398 274 147 411 2427 Fragments arranged by size: 2427 2164 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 1178 Size: 30 165 30 1362 30 4204 Fragments arranged by size: 4204 1362 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2661 1186 Size: 1475 4346 BseYI C'CCAG_C Cuts at: 2030 5024 5520 2030 Size: 2994 496 2331 Fragments arranged by size: 2994 2331 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 2995 1163 Size: 573 774 485 3989 Fragments arranged by size: 3989 774 573 485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3084 3099 3882 3967 5038 3084 Size: 15 783 85 1071 3867 Fragments arranged by size: 3867 1071 783 85 15 BsmI GAATG_Cn' Cuts at: 2616 3332 3425 2616 Size: 716 93 5012 Fragments arranged by size: 5012 716 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2335 3084 3099 3140 3882 3967 Size: 399 255 290 749 15 41 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 749 742 399 290 255 85 41 15 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 2629 3099 3710 5440 2629 Size: 470 611 1730 3010 Fragments arranged by size: 3010 1730 611 470 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 3176 844 Size: 1441 891 3489 Fragments arranged by size: 3489 1441 891 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2090 2676 2939 2976 4465 4652 Size: 110 207 73 586 263 37 1489 187 Cuts at: 4652 1700 Size: 2869 Fragments arranged by size: 2869 1489 586 263 207 187 110 73 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2532 1184 Size: 1348 4473 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2077 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2316 2962 3151 3518 3903 3920 5372 5458 Size: 344 646 189 367 385 17 1452 86 Cuts at: 5458 5470 5816 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 646 385 367 346 344 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2155 513 Size: 552 939 151 4179 Fragments arranged by size: 4179 939 552 151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2373 2613 3337 4131 4151 2373 Size: 240 724 794 20 4043 Fragments arranged by size: 4043 794 724 240 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2970 3005 529 Size: 35 2406 35 3345 Fragments arranged by size: 3345 2406 35 35 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2978 3268 4157 Size: 54 1425 50 275 18 1148 290 889 Cuts at: 4157 5786 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1148 889 290 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 2638 3751 1353 Size: 1274 11 1113 3423 Fragments arranged by size: 3423 1274 1113 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3097 3097 Size: 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 3006 3884 4481 5268 5536 1239 Size: 393 524 850 878 597 787 268 1524 Fragments arranged by size: 1524 878 850 787 597 524 393 268 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2037 2084 4968 1678 Size: 359 47 2884 2531 Fragments arranged by size: 2884 2531 359 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2947 2979 4462 4494 4536 Size: 32 135 32 1375 32 1483 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 1483 1375 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2386 3165 3411 669 Size: 988 281 448 779 246 3079 Fragments arranged by size: 3079 988 779 448 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4935 5119 1305 Size: 3630 184 2007 Fragments arranged by size: 3630 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3779 4138 4297 518 Size: 648 2613 359 159 2042 Fragments arranged by size: 2613 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3237 3914 5062 5307 1606 Size: 1631 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2155 513 Size: 552 939 151 4179 Fragments arranged by size: 4179 939 552 151 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2895 1108 Size: 1787 4034 PfoI T'CCnGG_A Cuts at: 2847 2847 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PmlI CAC'GTG Cuts at: 2953 2953 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 3003 3173 830 Size: 2173 170 3478 Fragments arranged by size: 3478 2173 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SacI G_AGCT'C Cuts at: 3099 3099 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 2972 4069 5781 2972 Size: 1097 1712 3012 Fragments arranged by size: 3012 1712 1097 SfcI C'TryA_G Cuts at: 826 1045 2999 3169 4304 4985 5176 826 Size: 219 1954 170 1135 681 191 1471 Fragments arranged by size: 1954 1471 1135 681 219 191 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 2039 3047 3148 1872 Size: 167 1008 101 4545 Fragments arranged by size: 4545 1008 167 101 SmlI C'TyrA_G Cuts at: 820 1017 1943 2966 3925 4826 5088 820 Size: 197 926 1023 959 901 262 1553 Fragments arranged by size: 1553 1023 959 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2004 2155 2326 2698 2760 3260 513 Size: 552 939 151 171 372 62 500 3074 Fragments arranged by size: 3074 939 552 500 372 171 151 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 2970 4067 Size: 274 80 33 51 1443 393 600 1097 Cuts at: 4067 5779 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1097 600 393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 5781 5813 2765 Size: 32 2984 32 2773 Fragments arranged by size: 2984 2773 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 2410 3157 2410 Size: 747 5074 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 2767 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 2037 3045 3146 1870 Size: 167 1008 101 4545 Fragments arranged by size: 4545 1008 167 101 XmnI GAAnn'nnTTC Cuts at: 2867 3950 2867 Size: 1083 4738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI