################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI Hin1I HindIII HpaI KasI KpnI Ksp632I MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 703 GTAC GT^AC AhdI 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 484 GATCC GGATC(4/5) AlwNI 313 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 90 CTCGGG C^[CT]CG[GA]G AviII 217 TGCGCA TGC^GCA BcgI 129 CGACGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 513 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 600 CTCCTC GAGGAG(10/8) BsmBI 459 CGTCTC CGTCTC(1/5) BsoBI 90 CTCGGG C^[CT]CG[GA]G Bsp1407I 702 TGTACA T^GTACA BspMI 377 ACCTGC ACCTGC(4/8) BsrGI 702 TGTACA T^GTACA BstAPI 312 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 484 GATC ^GATC DraII 102 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 472 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 327 CGGCCG [CT]^GGCC[GA] EagI 327 CGGCCG C^GGCCG Eam1105I 565 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 327 CGGCCG C^GGCCG Eco52I 327 CGGCCG C^GGCCG EcoO109I 102 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 830 CCAAGG C^C[AT][AT]GG FspI 217 TGCGCA TGC^GCA HphI 666 TCACC GGTGA(8/7) MaeIII 719 GTGAC ^GT[ACGT]AC MamI 513 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 484 GATC ^GATC NaeI 436 GCCGGC GCC^GGC NdeII 484 GATC ^GATC NgoMIV 436 GCCGGC G^CCGGC PflMI 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 102 GGGTCCC [GA]G^G[AT]CC[CT] PstI 310 CTGCAG CTGCA^G RsaI 703 GTAC GT^AC SacI 87 GAGCTC GAGCT^C Sau3AI 484 GATC ^GATC SfaNI 245 GCATC GCATC(5/9) SgrAI 65 CGCCGGTG C[GA]^CCGG[CT]G SspBI 702 TGTACA T^GTACA StyI 830 CCAAGG C^C[AT][AT]GG TfiI 663 GAATC G^A[AT]TC Tsp45I 719 GTGAC ^GT[GC]AC Van91I 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Alw44I 137 GTGCAC G^TGCAC 847 GTGCAC Aor51HI 257 AGCGCT AGC^GCT 551 AGCGCT ApaLI 137 GTGCAC G^TGCAC 847 GTGCAC AvaII 103 GGTCC G^G[AT]CC 631 GGTCC BbsI 424 GAAGAC GAAGAC(2/6) 652 GAAGAC BcnI 193 CCGGG CC^[GC]GG 474 CCGGG BfaI 350 CTAG C^TAG 410 CTAG BpmI 398 CTCCAG CTGGAG(16/14) 462 CTCCAG BpuAI 424 GAAGAC GAAGAC(2/6) 652 GAAGAC BsiEI 304 CGACCG CG[GA][CT]^CG 327 CGGCCG BsrFI 66 GCCGGT [GA]^CCGG[CT] 436 GCCGGC BsrI 141 ACTGG ACTGG(1/-1) 147 CCAGT BssHII 152 GCGCGC G^CGCGC 369 GCGCGC Cfr10I 66 GCCGGT [GA]^CCGG[CT] 436 GCCGGC Eco47III 257 AGCGCT AGC^GCT 551 AGCGCT EcoNI 757 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 811 CCTCTCCCAGG HgaI 130 GACGC GACGC(5/10) 341 GCGTC HincII 343 GTCAAC GT[CT]^[GA]AC 562 GTTGAC HindII 343 GTCAAC GT[CT]^[GA]AC 562 GTTGAC KspI 57 CCGCGG CCGC^GG 330 CCGCGG MaeI 350 CTAG C^TAG 410 CTAG MaeII 214 ACGT A^CGT 845 ACGT MboII 424 GAAGA GAAGA(8/7) 652 GAAGA NciI 193 CCGGG CC^[GC]GG 474 CCGGG NlaIII 535 CATG CATG^ 861 CATG NspI 534 GCATGC [GA]CATG^[CT] 860 GCATGC SacII 57 CCGCGG CCGC^GG 330 CCGCGG SfcI 310 CTGCAG C^T[GA][CT]AG 855 CTACAG SphI 534 GCATGC GCATG^C 860 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI AspHI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NlaIV PleI Sau96I ScrFI TseI TspRI