(Circular) (Six-base) MAPSORT of: pFN21AB6362.seq Check: 9132 from: 1 to: 5578 pFN21AB6362 5578 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2696 3641 5025 5293 1632 Size: 1064 945 1384 268 1917 Fragments arranged by size: 1917 1384 1064 945 268 AfeI AGC'GCT Cuts at: 2264 2558 2264 Size: 294 5284 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 2575 4307 2575 Size: 1732 3846 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2591 2623 2591 Size: 32 5546 AlwNI CAG_nnn'CTG Cuts at: 2323 3321 4893 2323 Size: 998 1572 3008 Fragments arranged by size: 3008 1572 998 ApaLI G'TGCA_C Cuts at: 2142 2852 3635 4791 2142 Size: 710 783 1156 2929 Fragments arranged by size: 2929 1156 783 710 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2903 1870 Size: 73 152 808 4545 Fragments arranged by size: 4545 808 152 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 4354 618 Size: 325 217 627 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2343 2459 2897 1391 Size: 705 247 116 438 4072 Fragments arranged by size: 4072 705 438 247 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2436 2664 928 Size: 971 537 228 3842 Fragments arranged by size: 3842 971 537 228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2123 2157 3769 3803 1903 Size: 34 50 34 102 34 1612 34 3678 Fragments arranged by size: 3678 1612 102 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2146 2856 3639 4795 1790 Size: 356 710 783 1156 2573 Fragments arranged by size: 2573 1156 783 710 356 BmgBI CAC'GTC Cuts at: 1318 2220 2851 1318 Size: 902 631 4045 Fragments arranged by size: 4045 902 631 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 3309 4267 Size: 416 53 83 399 24 729 1163 958 Cuts at: 4267 442 Size: 1753 Fragments arranged by size: 1753 1163 958 729 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2452 4238 1239 Size: 1149 64 1786 2579 Fragments arranged by size: 2579 1786 1149 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 4568 4866 3703 Size: 865 298 4415 Fragments arranged by size: 4415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 2522 5572 2522 Size: 3050 2528 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3385 3767 4402 275 Size: 53 83 186 936 1852 382 635 1451 Fragments arranged by size: 1852 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 2591 2621 1178 Size: 30 165 30 1041 30 117 30 4135 Fragments arranged by size: 4135 1041 165 117 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2596 1186 Size: 1410 4168 BseYI C'CCAG_C Cuts at: 4781 5277 4781 Size: 496 5082 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5005 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2312 2335 3028 3789 4817 1540 Size: 275 186 311 23 693 761 1028 2301 Fragments arranged by size: 2301 1028 761 693 311 275 186 23 BsiHKAI G_wGCw'C Cuts at: 2096 2146 2588 2856 3639 3724 4795 2096 Size: 50 442 268 783 85 1071 2879 Fragments arranged by size: 2879 1071 783 442 268 85 50 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 BsmBI CGTCTCn'nnnn_ Cuts at: 2470 2470 Size: 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2146 2343 2459 2588 2856 2897 Size: 399 306 50 197 116 129 268 41 Cuts at: 2897 3639 3724 4795 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 268 197 129 116 85 50 41 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2933 844 Size: 1547 542 3489 Fragments arranged by size: 3489 1547 542 BsrBI CCG'CTC Cuts at: 1394 1823 3465 1394 Size: 429 1642 3507 Fragments arranged by size: 3507 1642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4073 4247 65 Size: 1183 2825 174 1396 Fragments arranged by size: 2825 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 2441 4222 4409 1700 Size: 110 261 370 1781 187 2869 Fragments arranged by size: 2869 1781 370 261 187 110 BsrGI T'GTAC_A Cuts at: 96 2707 96 Size: 2611 2967 BssHII G'CGCG_C Cuts at: 1925 2157 2374 1925 Size: 232 217 5129 Fragments arranged by size: 5129 232 217 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 794 Size: 1529 4049 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573 Size: 936 367 385 17 1452 86 12 346 Cuts at: 5573 1972 Size: 1977 Fragments arranged by size: 1977 1452 936 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2062 2112 2335 513 Size: 552 997 50 223 3756 Fragments arranged by size: 3756 997 552 223 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2759 2854 3094 3888 3908 2452 Size: 307 95 240 794 20 4122 Fragments arranged by size: 4122 794 307 240 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2482 2482 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2332 3025 3914 Size: 54 1425 50 275 18 502 693 889 Cuts at: 3914 5543 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 693 502 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2332 3025 1537 Size: 275 520 693 4090 Fragments arranged by size: 4090 693 520 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3508 1353 Size: 2155 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2154 4165 4539 4685 387 Size: 1064 138 565 2011 374 146 1280 Fragments arranged by size: 2011 1280 1064 565 374 146 138 EcoICRI GAG'CTC Cuts at: 2094 2094 Size: 5578 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2452 2696 3641 4238 5025 5293 Size: 393 756 64 244 945 597 787 268 Cuts at: 5293 1239 Size: 1524 Fragments arranged by size: 1524 945 787 756 597 393 268 244 64 EcoNI CCTnn'n_nnAGG Cuts at: 2766 2820 2766 Size: 54 5524 EcoO109I rG'GnC_Cy Cuts at: 2108 2108 Size: 5578 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 5578 FspI TGC'GCA Cuts at: 2224 4084 2224 Size: 1860 3718 FspAI rTGC'GCAy Cuts at: 2224 2224 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2266 2324 2396 2442 2560 4725 1678 Size: 588 58 72 46 118 2165 2531 Fragments arranged by size: 2531 2165 588 118 72 58 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2444 2476 2561 Size: 32 135 32 438 32 402 32 85 Cuts at: 2561 2593 4219 4251 4293 4325 1373 Size: 32 1626 32 42 32 2626 Fragments arranged by size: 2626 1626 438 402 135 85 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 2569 2922 3168 669 Size: 988 281 412 219 353 246 3079 Fragments arranged by size: 3079 988 412 353 281 246 219 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 4692 4876 1305 Size: 1098 2289 184 2007 Fragments arranged by size: 2289 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3536 3895 4054 518 Size: 648 2370 359 159 2042 Fragments arranged by size: 2370 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2036 2064 2314 2337 2994 3671 4819 Size: 405 25 28 250 23 657 677 1148 Cuts at: 4819 5064 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 657 405 250 245 28 25 23 NaeI GCC'GGC Cuts at: 2443 2443 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5026 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2441 2441 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2543 2869 2936 4481 1132 Size: 97 1314 326 67 1545 2229 Fragments arranged by size: 2229 1545 1314 326 97 67 PasI CC'CwG_GG Cuts at: 1348 2700 1348 Size: 1352 4226 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 1108 Size: 920 4658 PfoI T'CCnGG_A Cuts at: 2404 2404 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2591 2623 3647 3679 5160 5192 2591 Size: 32 1024 32 1481 32 2977 Fragments arranged by size: 2977 1481 1024 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2108 2108 Size: 5578 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 2742 3148 2742 Size: 406 5172 PstI C_TGCA'G Cuts at: 830 2319 2930 830 Size: 1489 611 3478 Fragments arranged by size: 3478 1489 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 SacI G_AGCT'C Cuts at: 2096 2096 Size: 5578 SacII CC_GC'GG Cuts at: 2065 2338 2065 Size: 273 5305 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SanDI GG'GwC_CC Cuts at: 2108 2108 Size: 5578 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2315 2860 2926 4061 4742 4933 826 Size: 219 1270 545 66 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 545 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 2071 1700 Size: 371 5207 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 4583 4845 820 Size: 197 926 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2543 2869 2936 1132 Size: 1411 326 67 3774 Fragments arranged by size: 3774 1411 326 67 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2835 3017 513 Size: 552 1770 182 3074 Fragments arranged by size: 3074 1770 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2707 3824 5536 Size: 274 80 33 51 1443 730 1117 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1712 1443 1117 730 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI