(Circular) (Six-base) MAPSORT of: pFN21AE1065.seq Check: 56 from: 1 to: 7669 pFN21AE1065 7669 bp With 182 enzymes: SgfI * February 27, 2009 15:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2972 2972 Size: 7669 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5479 278 Size: 53 83 186 936 3943 2468 Fragments arranged by size: 3943 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2100 5012 1937 Size: 163 2912 4594 Fragments arranged by size: 4594 2912 163 Acc65I G'GTAC_C Cuts at: 4990 4990 Size: 7669 AclI AA'CG_TT Cuts at: 1563 5796 6169 1563 Size: 4233 373 3063 Fragments arranged by size: 4233 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5732 7116 7384 1632 Size: 4100 1384 268 1917 Fragments arranged by size: 4100 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7472 AflIII A'CryG_T Cuts at: 1203 2678 4041 6568 7545 1203 Size: 1475 1363 2527 977 1327 Fragments arranged by size: 2527 1475 1363 1327 977 AgeI A'CCGG_T Cuts at: 6500 6500 Size: 7669 AhdI GACnn_n'nnGTC Cuts at: 6398 6398 Size: 7669 AleI CACnn'nnGTG Cuts at: 1166 2652 2789 3921 1166 Size: 1486 137 1132 4914 Fragments arranged by size: 4914 1486 1132 137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3404 1184 Size: 2220 5449 AlwNI CAG_nnn'CTG Cuts at: 2786 3043 3547 3736 3806 5412 6984 2786 Size: 257 504 189 70 1606 1572 3471 Fragments arranged by size: 3471 1606 1572 504 257 189 70 ApaI G_GGCC'C Cuts at: 3256 3426 3256 Size: 170 7499 ApaLI G'TGCA_C Cuts at: 4226 5726 6882 4226 Size: 1500 1156 5013 Fragments arranged by size: 5013 1500 1156 ApoI r'AATT_y Cuts at: 1493 4809 4978 5204 5356 7465 1493 Size: 3316 169 226 152 2109 1697 Fragments arranged by size: 3316 2109 1697 226 169 152 AseI AT'TA_AT Cuts at: 160 1032 6223 160 Size: 872 5191 1606 Fragments arranged by size: 5191 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7669 AvaI C'yCGr_G Cuts at: 1870 1943 2287 3025 3172 3249 3419 4139 4301 Size: 73 344 738 147 77 170 720 162 Cuts at: 4301 4994 1870 Size: 693 4545 Fragments arranged by size: 4545 738 720 693 344 170 162 147 77 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2287 2320 2287 Size: 33 7636 BamHI G'GATC_C Cuts at: 4509 4671 4999 5366 4509 Size: 162 328 367 6812 Fragments arranged by size: 6812 367 328 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3404 3606 3911 4031 4193 Size: 325 217 627 1617 202 305 120 162 Cuts at: 4193 4289 4779 4990 6445 618 Size: 96 490 211 1455 1842 Fragments arranged by size: 1842 1617 1455 627 490 325 305 217 211 202 162 120 96 BanII G_rGCy'C Cuts at: 1391 2088 3256 3426 3933 4140 4988 1391 Size: 697 1168 170 507 207 848 4072 Fragments arranged by size: 4072 1168 848 697 507 207 170 BbeI G_GCGC'C Cuts at: 3610 4783 3610 Size: 1173 6496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2229 2843 928 Size: 971 330 614 5754 Fragments arranged by size: 5754 971 614 330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2710 2744 2784 2818 3751 3785 3901 Size: 34 773 34 40 34 933 34 116 Cuts at: 3901 3935 4336 4370 5860 5894 1903 Size: 34 401 34 1490 34 3678 Fragments arranged by size: 3678 1490 933 773 401 116 40 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5561 6771 5561 Size: 1210 6459 BclI T'GATC_A Cuts at: 1548 4830 1548 Size: 3282 4387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2439 2934 4119 Size: 107 122 71 631 605 767 495 1185 Cuts at: 4119 4287 6280 136 Size: 168 1993 1525 Fragments arranged by size: 1993 1525 1185 767 631 605 495 168 122 107 71 BglII A'GATC_T Cuts at: 2394 4530 7664 2394 Size: 2136 3134 2399 Fragments arranged by size: 3134 2399 2136 BlpI GC'TnA_GC Cuts at: 5086 5086 Size: 7669 Bme1580I G_kGCm'C Cuts at: 1790 2057 2126 2527 3256 3426 4004 4036 4124 Size: 267 69 401 729 170 578 32 88 Cuts at: 4124 4230 4722 5730 6886 1790 Size: 106 492 1008 1156 2573 Fragments arranged by size: 2573 1156 1008 729 578 492 401 267 170 106 88 69 32 BmgBI CAC'GTC Cuts at: 1318 2104 2736 4596 4849 1318 Size: 786 632 1860 253 4138 Fragments arranged by size: 4138 1860 786 632 253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5400 6358 442 Size: 416 53 83 399 24 3983 958 1753 Fragments arranged by size: 3983 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7669 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4605 4605 Size: 7669 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3863 4053 6329 1239 Size: 2624 190 2276 2579 Fragments arranged by size: 2624 2579 2276 190 Bpu10I CC'TnA_GC Cuts at: 3353 4134 3353 Size: 781 6888 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3566 5794 6659 6957 3566 Size: 2228 865 298 4278 Fragments arranged by size: 4278 2228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3524 3802 4607 6332 882 Size: 617 2025 278 805 1725 2219 Fragments arranged by size: 2219 2025 1725 805 617 278 BsaAI yAC'GTr Cuts at: 493 1204 2679 2724 4125 5460 493 Size: 711 1475 45 1401 1335 2702 Fragments arranged by size: 2702 1475 1401 1335 711 45 BsaBI GATnn'nnATC Cuts at: 4514 7663 4514 Size: 3149 4520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2543 3607 4780 5476 Size: 53 83 186 936 1010 1064 1173 696 Cuts at: 5476 5858 6493 275 Size: 382 635 1451 Fragments arranged by size: 1451 1173 1064 1010 936 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2763 6102 6500 6774 6921 7332 2763 Size: 3339 398 274 147 411 3100 Fragments arranged by size: 3339 3100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2969 2999 1178 Size: 30 165 30 1566 30 5848 Fragments arranged by size: 5848 1566 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3583 1186 Size: 2397 5272 BseYI C'CCAG_C Cuts at: 3255 3425 4004 4459 6872 7368 3255 Size: 170 579 455 2413 496 3556 Fragments arranged by size: 3556 2413 579 496 455 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2593 3121 1163 Size: 573 857 528 5711 Fragments arranged by size: 5711 857 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2719 2764 3775 4086 5119 Size: 275 186 18 700 45 1011 311 1033 Cuts at: 5119 5880 6908 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1033 1028 1011 761 700 311 275 186 45 18 BsiHKAI G_wGCw'C Cuts at: 2117 2987 3933 4230 5730 5815 6886 2117 Size: 870 946 297 1500 85 1071 2900 Fragments arranged by size: 2900 1500 1071 946 870 297 85 BsmI GAATG_Cn' Cuts at: 4478 5180 5273 4478 Size: 702 93 6874 Fragments arranged by size: 6874 702 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2534 2726 2534 Size: 192 7477 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2088 2117 2126 2527 2987 3256 Size: 399 267 31 29 9 401 460 269 Cuts at: 3256 3426 3933 4004 4036 4124 4140 4230 4722 Size: 170 507 71 32 88 16 90 492 Cuts at: 4722 4988 5730 5815 6886 1391 Size: 266 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 507 492 460 401 399 269 267 266 170 90 88 85 71 32 31 29 16 9 BspEI T'CCGG_A Cuts at: 7332 7332 Size: 7669 BspHI T'CATG_A Cuts at: 4572 5558 7288 4572 Size: 986 1730 4953 Fragments arranged by size: 4953 1730 986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 2477 2972 4625 5024 844 Size: 1471 162 495 1653 399 3489 Fragments arranged by size: 3489 1653 1471 495 399 162 BsrBI CCG'CTC Cuts at: 1394 1823 2481 2995 4656 5556 1394 Size: 429 658 514 1661 900 3507 Fragments arranged by size: 3507 1661 900 658 514 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6164 6338 65 Size: 1183 4916 174 1396 Fragments arranged by size: 4916 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3774 4148 4421 6313 6500 1700 Size: 110 1964 374 273 1892 187 2869 Fragments arranged by size: 2869 1964 1892 374 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7669 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7669 BssSI C'ACGA_G Cuts at: 2270 3519 5729 6741 2270 Size: 1249 2210 1012 3198 Fragments arranged by size: 3198 2210 1249 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3547 3999 4152 4278 794 Size: 2753 452 153 126 4185 Fragments arranged by size: 4185 2753 452 153 126 BstBI TT'CG_AA Cuts at: 6473 6473 Size: 7669 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7669 BstXI CCAn_nnnn'nTGG Cuts at: 3841 3923 3841 Size: 82 7587 BstYI r'GATC_y Cuts at: 1972 2394 3477 4509 4530 4671 4999 5366 5751 Size: 422 1083 1032 21 141 328 367 385 Cuts at: 5751 5768 7220 7306 7318 7664 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1083 1032 422 385 367 346 328 141 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 2515 2904 3052 3610 4116 Size: 552 939 405 106 389 148 558 506 Cuts at: 4116 4910 513 Size: 794 3272 Fragments arranged by size: 3272 939 794 558 552 506 405 389 148 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2809 4471 4575 4624 4788 487 Size: 683 56 1583 1662 104 49 164 3368 Fragments arranged by size: 3368 1662 1583 683 164 104 56 49 BtsI GCAGTG_nn' Cuts at: 2222 2819 3079 3556 4228 4318 4378 4393 5185 Size: 597 260 477 672 90 60 15 792 Cuts at: 5185 5979 5999 2222 Size: 794 20 3892 Fragments arranged by size: 3892 794 792 672 597 477 260 90 60 20 15 ClaI AT'CG_AT Cuts at: 1554 2142 1554 Size: 588 7081 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2362 2397 529 Size: 35 1798 35 5801 Fragments arranged by size: 5801 1798 35 35 DraI TTT'AAA Cuts at: 4973 4973 Size: 7669 DraIII CAC_nnn'GTG Cuts at: 3999 3999 Size: 7669 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6676 809 Size: 507 5360 1802 Fragments arranged by size: 5360 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2070 3613 Size: 54 1425 50 275 18 186 54 1543 Cuts at: 3613 3772 3934 4083 4240 4309 5116 6005 7634 Size: 159 162 149 157 69 807 889 1629 Cuts at: 7634 8 Size: 43 Fragments arranged by size: 1629 1543 1425 889 807 275 186 162 159 157 149 69 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 3772 4083 5116 1537 Size: 275 204 1756 311 1033 4090 Fragments arranged by size: 4090 1756 1033 311 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 3326 3596 3675 4574 4847 5599 1353 Size: 1973 270 79 899 273 752 3423 Fragments arranged by size: 3423 1973 899 752 273 270 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6256 6630 6776 387 Size: 1064 138 4667 374 146 1280 Fragments arranged by size: 4667 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3931 3931 Size: 7669 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3863 4053 5732 6329 7116 7384 1239 Size: 393 2231 190 1679 597 787 268 1524 Fragments arranged by size: 2231 1679 1524 787 597 393 268 190 EcoNI CCTnn'n_nnAGG Cuts at: 4630 4630 Size: 7669 EcoO109I rG'GnC_Cy Cuts at: 2672 4837 2672 Size: 2165 5504 EcoRI G'AATT_C Cuts at: 4978 5356 4978 Size: 378 7291 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3487 4858 3487 Size: 1371 6298 FspI TGC'GCA Cuts at: 2740 3761 6175 2740 Size: 1021 2414 4234 Fragments arranged by size: 4234 2414 1021 HaeII r_GCGC'y Cuts at: 1678 3389 3610 3842 4783 6816 1678 Size: 1711 221 232 941 2033 2531 Fragments arranged by size: 2531 2033 1711 941 232 221 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 2969 3001 6310 Size: 32 135 32 527 32 838 32 3309 Cuts at: 6310 6342 6384 6416 1373 Size: 32 42 32 2626 Fragments arranged by size: 3309 2626 838 527 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2101 2869 3139 5013 5259 669 Size: 988 281 163 768 270 1874 246 3079 Fragments arranged by size: 3079 1874 988 768 281 270 246 163 HindIII A'AGCT_T Cuts at: 748 748 Size: 7669 HpaI GTT'AAC Cuts at: 5259 5259 Size: 7669 KasI G'GCGC_C Cuts at: 3606 4779 3606 Size: 1173 6496 KpnI G_GTAC'C Cuts at: 4994 4994 Size: 7669 MfeI C'AATT_G Cuts at: 5268 5268 Size: 7669 MluI A'CGCG_T Cuts at: 7545 7545 Size: 7669 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2791 4026 6783 6967 1305 Size: 1486 1235 2757 184 2007 Fragments arranged by size: 2757 2007 1486 1235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4311 10 Size: 54 1425 343 2479 3368 Fragments arranged by size: 3368 2479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2520 2652 2789 3561 3921 5627 Size: 648 976 378 132 137 772 360 1706 Cuts at: 5627 5986 6145 518 Size: 359 159 2042 Fragments arranged by size: 2042 1706 976 772 648 378 360 359 159 137 132 MspA1I CmG'CkG Cuts at: 1606 2082 2652 2802 3054 3259 3429 3547 4269 Size: 476 570 150 252 205 170 118 722 Cuts at: 4269 4278 4701 5085 5762 6910 7155 1606 Size: 9 423 384 677 1148 245 2120 Fragments arranged by size: 2120 1148 722 677 570 476 423 384 252 245 205 170 150 118 9 NaeI GCC'GGC Cuts at: 3776 3776 Size: 7669 NarI GG'CG_CC Cuts at: 3607 4780 3607 Size: 1173 6496 NcoI C'CATG_G Cuts at: 513 1065 2004 2409 2515 513 Size: 552 939 405 106 5667 Fragments arranged by size: 5667 939 552 405 106 NdeI CA'TA_TG Cuts at: 387 387 Size: 7669 NgoMIV G'CCGG_C Cuts at: 3774 3774 Size: 7669 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7669 NotI GC'GGCC_GC Cuts at: 5116 5116 Size: 7669 NspI r_CATG'y Cuts at: 1132 1229 2772 2844 3699 3861 5027 6572 1132 Size: 97 1543 72 855 162 1166 1545 2229 Fragments arranged by size: 2229 1545 1543 1166 855 162 97 72 PasI CC'CwG_GG Cuts at: 1348 2050 2090 1348 Size: 702 40 6927 Fragments arranged by size: 6927 702 40 PciI A'CATG_T Cuts at: 6568 6568 Size: 7669 PflMI CCAn_nnn'nTGG Cuts at: 1108 3973 1108 Size: 2865 4804 PfoI T'CCnGG_A Cuts at: 4206 4206 Size: 7669 PmeI GTTT'AAAC Cuts at: 4973 4973 Size: 7669 PmlI CAC'GTG Cuts at: 2679 2724 2679 Size: 45 7624 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5738 5770 7251 7283 5738 Size: 32 1481 32 6124 Fragments arranged by size: 6124 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2364 1613 Size: 751 6918 PsiI TTA'TAA Cuts at: 5239 5239 Size: 7669 PspOMI G'GGCC_C Cuts at: 3252 3422 3252 Size: 170 7499 PstI C_TGCA'G Cuts at: 830 2573 2826 3269 3393 3741 5021 830 Size: 1743 253 443 124 348 1280 3478 Fragments arranged by size: 3478 1743 1280 443 348 253 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7669 PvuII CAG'CTG Cuts at: 2652 2802 3259 3429 2652 Size: 150 457 170 6892 Fragments arranged by size: 6892 457 170 150 SacI G_AGCT'C Cuts at: 3933 3933 Size: 7669 SacII CC_GC'GG Cuts at: 3055 3055 Size: 7669 SalI G'TCGA_C Cuts at: 1936 2099 5011 1936 Size: 163 2912 4594 Fragments arranged by size: 4594 2912 163 SbfI CC_TGCA'GG Cuts at: 3741 5021 3741 Size: 1280 6389 ScaI AGT'ACT Cuts at: 5917 7629 5917 Size: 1712 5957 SfcI C'TryA_G Cuts at: 826 1045 2296 2569 2822 3265 3389 3737 3878 Size: 219 1251 273 253 443 124 348 141 Cuts at: 3878 5017 6152 6833 7024 826 Size: 1139 1135 681 191 1471 Fragments arranged by size: 1471 1251 1139 1135 681 443 348 273 253 219 191 141 124 SfoI GGC'GCC Cuts at: 3608 4781 3608 Size: 1173 6496 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7669 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7669 SmaI CCC'GGG Cuts at: 1872 2289 3027 3174 3251 4141 4996 1872 Size: 417 738 147 77 890 855 4545 Fragments arranged by size: 4545 890 855 738 417 147 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 3581 5773 6674 6936 820 Size: 197 926 1638 2192 901 262 1553 Fragments arranged by size: 2192 1638 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5460 493 Size: 4967 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7669 SphI G_CATG'C Cuts at: 1132 5027 1132 Size: 3895 3774 SrfI GCCC'GGGC Cuts at: 4141 4141 Size: 7669 SspI AAT'ATT Cuts at: 5 52 5593 5 Size: 47 5541 2081 Fragments arranged by size: 5541 2081 47 StuI AGG'CCT Cuts at: 1517 3686 4109 1517 Size: 2169 423 5077 Fragments arranged by size: 5077 2169 423 StyI C'CwwG_G Cuts at: 513 1065 2004 2409 2515 3144 3630 3834 4896 Size: 552 939 405 106 629 486 204 1062 Cuts at: 4896 5108 513 Size: 212 3074 Fragments arranged by size: 3074 1062 939 629 552 486 405 212 204 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3929 4277 5696 5713 5866 6051 6496 Size: 1255 2349 348 1419 17 153 185 445 Cuts at: 6496 325 Size: 1498 Fragments arranged by size: 2349 1498 1419 1255 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4878 5915 7627 Size: 274 80 33 51 1443 2901 1037 1712 Cuts at: 7627 96 Size: 138 Fragments arranged by size: 2901 1712 1443 1037 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7629 7661 7629 Size: 32 7637 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7669 XbaI T'CTAG_A Cuts at: 5005 5005 Size: 7669 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 4304 2554 Size: 1750 5919 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7669 XmaI C'CCGG_G Cuts at: 1870 2287 3025 3172 3249 4139 4994 1870 Size: 417 738 147 77 890 855 4545 Fragments arranged by size: 4545 890 855 738 417 147 77 XmnI GAAnn'nnTTC Cuts at: 4871 5798 4871 Size: 927 6742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5477 276 Size: 53 83 186 936 3943 2468 Fragments arranged by size: 3943 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SwaI