Restriction map for
pFN21AE1065
(Linear) (Six-base) MAPSORT of: KIEE1065.seq Check: 8845 from: 1 to: 2964
KIEE1065.orf
With 182 enzymes: SgfI *
February 26, 2009 15:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 967 2964
Size: 967 1997
AccI GT'mk_AC
Cuts at: 0 95 2964
Size: 95 2869
AflIII A'CryG_T
Cuts at: 0 673 2036 2964
Size: 673 1363 928
Fragments arranged by size:
1363 928 673
AleI CACnn'nnGTG
Cuts at: 0 647 784 1916 2964
Size: 647 137 1132 1048
Fragments arranged by size:
1132 1048 647 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1399 2964
Size: 1399 1565
AlwNI CAG_nnn'CTG
Cuts at: 0 781 1038 1542 1731 1801 2964
Size: 781 257 504 189 70 1163
Fragments arranged by size:
1163 781 504 257 189 70
ApaI G_GGCC'C
Cuts at: 0 1251 1421 2964
Size: 1251 170 1543
Fragments arranged by size:
1543 1251 170
ApaLI G'TGCA_C
Cuts at: 0 2221 2964
Size: 2221 743
ApoI r'AATT_y
Cuts at: 0 2804 2964
Size: 2804 160
AvaI C'yCGr_G
Cuts at: 0 282 1020 1167 1244 1414 2134 2296 2964
Size: 282 738 147 77 170 720 162 668
Fragments arranged by size:
738 720 668 282 170 162 147 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 282 315 2964
Size: 282 33 2649
Fragments arranged by size:
2649 282 33
BamHI G'GATC_C
Cuts at: 0 2504 2666 2964
Size: 2504 162 298
Fragments arranged by size:
2504 298 162
BanI G'GyrC_C
Cuts at: 0 1399 1601 1906 2026 2188 2284 2774 2964
Size: 1399 202 305 120 162 96 490 190
Fragments arranged by size:
1399 490 305 202 190 162 120 96
BanII G_rGCy'C
Cuts at: 0 83 1251 1421 1928 2135 2964
Size: 83 1168 170 507 207 829
Fragments arranged by size:
1168 829 507 207 170 83
BbeI G_GCGC'C
Cuts at: 0 1605 2778 2964
Size: 1605 1173 186
Fragments arranged by size:
1605 1173 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 224 838 2964
Size: 224 614 2126
Fragments arranged by size:
2126 614 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 705 739 779 813 1746 1780 1896 1930
Size: 705 34 40 34 933 34 116 34
Cuts at: 1930 2331 2365 2964
Size: 401 34 599
Fragments arranged by size:
933 705 599 401 116 40 34 34
34 34 34
BclI T'GATC_A
Cuts at: 0 2825 2964
Size: 2825 139
BglI GCCn_nnn'nGGC
Cuts at: 0 434 929 2114 2282 2964
Size: 434 495 1185 168 682
Fragments arranged by size:
1185 682 495 434 168
BglII A'GATC_T
Cuts at: 0 389 2525 2964
Size: 389 2136 439
Fragments arranged by size:
2136 439 389
Bme1580I G_kGCm'C
Cuts at: 0 52 121 522 1251 1421 1999 2031 2119
Size: 52 69 401 729 170 578 32 88
Cuts at: 2119 2225 2717 2964
Size: 106 492 247
Fragments arranged by size:
729 578 492 401 247 170 106 88
69 52 32
BmgBI CAC'GTC
Cuts at: 0 99 731 2591 2844 2964
Size: 99 632 1860 253 120
Fragments arranged by size:
1860 632 253 120 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2600 2964
Size: 2600 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1858 2048 2964
Size: 1858 190 916
Fragments arranged by size:
1858 916 190
Bpu10I CC'TnA_GC
Cuts at: 0 1348 2129 2964
Size: 1348 781 835
Fragments arranged by size:
1348 835 781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 2964
Size: 1561 1403
BsaI GGTCTCn'nnnn_
Cuts at: 0 1519 1797 2602 2964
Size: 1519 278 805 362
Fragments arranged by size:
1519 805 362 278
BsaAI yAC'GTr
Cuts at: 0 674 719 2120 2964
Size: 674 45 1401 844
Fragments arranged by size:
1401 844 674 45
BsaBI GATnn'nnATC
Cuts at: 0 2509 2964
Size: 2509 455
BsaHI Gr'CG_yC
Cuts at: 0 538 1602 2775 2964
Size: 538 1064 1173 189
Fragments arranged by size:
1173 1064 538 189
BsaWI w'CCGG_w
Cuts at: 0 758 2964
Size: 758 2206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 964 994 2964
Size: 964 30 1970
Fragments arranged by size:
1970 964 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1578 2964
Size: 1578 1386
BseYI C'CCAG_C
Cuts at: 0 1250 1420 1999 2454 2964
Size: 1250 170 579 455 510
Fragments arranged by size:
1250 579 510 455 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1116 2964
Size: 588 528 1848
Fragments arranged by size:
1848 588 528
BsiEI CG_ry'CG
Cuts at: 0 14 714 759 1770 2081 2964
Size: 14 700 45 1011 311 883
Fragments arranged by size:
1011 883 700 311 45 14
BsiHKAI G_wGCw'C
Cuts at: 0 112 982 1928 2225 2964
Size: 112 870 946 297 739
Fragments arranged by size:
946 870 739 297 112
BsmI GAATG_Cn'
Cuts at: 0 2473 2964
Size: 2473 491
BsmBI CGTCTCn'nnnn_
Cuts at: 0 529 721 2964
Size: 529 192 2243
Fragments arranged by size:
2243 529 192
Bsp1286I G_dGCh'C
Cuts at: 0 52 83 112 121 522 982 1251 1421
Size: 52 31 29 9 401 460 269 170
Cuts at: 1421 1928 1999 2031 2119 2135 2225 2717 2964
Size: 507 71 32 88 16 90 492 247
Fragments arranged by size:
507 492 460 401 269 247 170 90
88 71 52 32 31 29 16 9
BspHI T'CATG_A
Cuts at: 0 2567 2964
Size: 2567 397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 310 472 967 2620 2964
Size: 310 162 495 1653 344
Fragments arranged by size:
1653 495 344 310 162
BsrBI CCG'CTC
Cuts at: 0 476 990 2651 2964
Size: 476 514 1661 313
Fragments arranged by size:
1661 514 476 313
BsrFI r'CCGG_y
Cuts at: 0 1769 2143 2416 2964
Size: 1769 374 273 548
Fragments arranged by size:
1769 548 374 273
BssSI C'ACGA_G
Cuts at: 0 265 1514 2964
Size: 265 1249 1450
Fragments arranged by size:
1450 1249 265
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1542 1994 2147 2273 2964
Size: 1542 452 153 126 691
Fragments arranged by size:
1542 691 452 153 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1836 1918 2964
Size: 1836 82 1046
Fragments arranged by size:
1836 1046 82
BstYI r'GATC_y
Cuts at: 0 389 1472 2504 2525 2666 2964
Size: 389 1083 1032 21 141 298
Fragments arranged by size:
1083 1032 389 298 141 21
BtgI C'CryG_G
Cuts at: 0 404 510 899 1047 1605 2111 2905 2964
Size: 404 106 389 148 558 506 794 59
Fragments arranged by size:
794 558 506 404 389 148 106 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 804 2466 2570 2619 2783 2964
Size: 804 1662 104 49 164 181
Fragments arranged by size:
1662 804 181 164 104 49
BtsI GCAGTG_nn'
Cuts at: 0 217 814 1074 1551 2223 2313 2373 2388
Size: 217 597 260 477 672 90 60 15
Cuts at: 2388 2964
Size: 576
Fragments arranged by size:
672 597 576 477 260 217 90 60
15
ClaI AT'CG_AT
Cuts at: 0 137 2964
Size: 137 2827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 357 392 2964
Size: 357 35 2572
Fragments arranged by size:
2572 357 35
DraIII CAC_nnn'GTG
Cuts at: 0 1994 2964
Size: 1994 970
EaeI y'GGCC_r
Cuts at: 0 11 65 1608 1767 1929 2078 2235 2304
Size: 11 54 1543 159 162 149 157 69
Cuts at: 2304 2964
Size: 660
Fragments arranged by size:
1543 660 162 159 157 149 69 54
11
EagI C'GGCC_G
Cuts at: 0 11 1767 2078 2964
Size: 11 1756 311 886
Fragments arranged by size:
1756 886 311 11
EarI CTCTTCn'nnn_
Cuts at: 0 1321 1591 1670 2569 2842 2964
Size: 1321 270 79 899 273 122
Fragments arranged by size:
1321 899 273 270 122 79
EcoICRI GAG'CTC
Cuts at: 0 1926 2964
Size: 1926 1038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1858 2048 2964
Size: 1858 190 916
Fragments arranged by size:
1858 916 190
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2625 2964
Size: 2625 339
EcoO109I rG'GnC_Cy
Cuts at: 0 667 2832 2964
Size: 667 2165 132
Fragments arranged by size:
2165 667 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1482 2853 2964
Size: 1482 1371 111
Fragments arranged by size:
1482 1371 111
FspI TGC'GCA
Cuts at: 0 735 1756 2964
Size: 735 1021 1208
Fragments arranged by size:
1208 1021 735
HaeII r_GCGC'y
Cuts at: 0 1384 1605 1837 2778 2964
Size: 1384 221 232 941 186
Fragments arranged by size:
1384 941 232 221 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 964 996 2964
Size: 94 32 838 32 1968
Fragments arranged by size:
1968 838 94 32 32
HincII GTy'rAC
Cuts at: 0 96 864 1134 2964
Size: 96 768 270 1830
Fragments arranged by size:
1830 768 270 96
KasI G'GCGC_C
Cuts at: 0 1601 2774 2964
Size: 1601 1173 190
Fragments arranged by size:
1601 1173 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 2021 2964
Size: 786 1235 943
Fragments arranged by size:
1235 943 786
MscI TGG'CCA
Cuts at: 0 2306 2964
Size: 2306 658
MslI CAynn'nnrTG
Cuts at: 0 137 515 647 784 1556 1916 2964
Size: 137 378 132 137 772 360 1048
Fragments arranged by size:
1048 772 378 360 137 137 132
MspA1I CmG'CkG
Cuts at: 0 77 647 797 1049 1254 1424 1542 2264
Size: 77 570 150 252 205 170 118 722
Cuts at: 2264 2273 2696 2964
Size: 9 423 268
Fragments arranged by size:
722 570 423 268 252 205 170 150
118 77 9
NaeI GCC'GGC
Cuts at: 0 1771 2964
Size: 1771 1193
NarI GG'CG_CC
Cuts at: 0 1602 2775 2964
Size: 1602 1173 189
Fragments arranged by size:
1602 1173 189
NcoI C'CATG_G
Cuts at: 0 404 510 2964
Size: 404 106 2454
Fragments arranged by size:
2454 404 106
NgoMIV G'CCGG_C
Cuts at: 0 1769 2964
Size: 1769 1195
NspI r_CATG'y
Cuts at: 0 767 839 1694 1856 2964
Size: 767 72 855 162 1108
Fragments arranged by size:
1108 855 767 162 72
PasI CC'CwG_GG
Cuts at: 0 45 85 2964
Size: 45 40 2879
Fragments arranged by size:
2879 45 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 1968 2964
Size: 1968 996
PfoI T'CCnGG_A
Cuts at: 0 2201 2964
Size: 2201 763
PmlI CAC'GTG
Cuts at: 0 674 719 2964
Size: 674 45 2245
Fragments arranged by size:
2245 674 45
PshAI GACnn'nnGTC
Cuts at: 0 359 2964
Size: 359 2605
PspOMI G'GGCC_C
Cuts at: 0 1247 1417 2964
Size: 1247 170 1547
Fragments arranged by size:
1547 1247 170
PstI C_TGCA'G
Cuts at: 0 568 821 1264 1388 1736 2964
Size: 568 253 443 124 348 1228
Fragments arranged by size:
1228 568 443 348 253 124
PvuII CAG'CTG
Cuts at: 0 647 797 1254 1424 2964
Size: 647 150 457 170 1540
Fragments arranged by size:
1540 647 457 170 150
SacI G_AGCT'C
Cuts at: 0 1928 2964
Size: 1928 1036
SacII CC_GC'GG
Cuts at: 0 1050 2964
Size: 1050 1914
SalI G'TCGA_C
Cuts at: 0 94 2964
Size: 94 2870
SbfI CC_TGCA'GG
Cuts at: 0 1736 2964
Size: 1736 1228
SfcI C'TryA_G
Cuts at: 0 291 564 817 1260 1384 1732 1873 2964
Size: 291 273 253 443 124 348 141 1091
Fragments arranged by size:
1091 443 348 291 273 253 141 124
SfoI GGC'GCC
Cuts at: 0 1603 2776 2964
Size: 1603 1173 188
Fragments arranged by size:
1603 1173 188
SmaI CCC'GGG
Cuts at: 0 284 1022 1169 1246 2136 2964
Size: 284 738 147 77 890 828
Fragments arranged by size:
890 828 738 284 147 77
SmlI C'TyrA_G
Cuts at: 0 1576 2964
Size: 1576 1388
SrfI GCCC'GGGC
Cuts at: 0 2136 2964
Size: 2136 828
StuI AGG'CCT
Cuts at: 0 1681 2104 2964
Size: 1681 423 860
Fragments arranged by size:
1681 860 423
StyI C'CwwG_G
Cuts at: 0 404 510 1139 1625 1829 2891 2964
Size: 404 106 629 486 204 1062 73
Fragments arranged by size:
1062 629 486 404 204 106 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1924 2272 2964
Size: 1924 348 692
Fragments arranged by size:
1924 692 348
TatI w'GTAC_w
Cuts at: 0 2873 2964
Size: 2873 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 2299 2964
Size: 549 1750 665
Fragments arranged by size:
1750 665 549
XmaI C'CCGG_G
Cuts at: 0 282 1020 1167 1244 2134 2964
Size: 282 738 147 77 890 830
Fragments arranged by size:
890 830 738 282 147 77
XmnI GAAnn'nnTTC
Cuts at: 0 2866 2964
Size: 2866 98
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BclI BglI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PshAI PspOMI PstI PvuII SacI SacII SalI SbfI
SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BbvCI BciVI BfrBI
BlpI BmrI BmtI BsiWI BspEI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I DraI DrdI EciI EcoRI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TstI Tth111I XbaI XhoI ZraI