Restriction map for
pFN21AB9130
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KspI MaeII MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 369 GGTACC G^GTACC
AflII 1813 CTTAAG C^TTAAG
AlwI 1972 GATCC GGATC(4/5)
AseI 1036 ATTAAT AT^TAAT
AsnI 1036 ATTAAT AT^TAAT
Asp718I 369 GGTACC G^GTACC
BalI 1989 TGGCCA TGG^CCA
BanII 1897 GAGCTC G[GA]GC[CT]^C
BfrI 1813 CTTAAG C^TTAAG
BsaBI 322 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 113 GAGACC GGTCTC(1/5)
BsmFI 1700 GTCCC GGGAC(10/14)
BsmI 851 GAATGC GAATGC(1/-1)
Bsp1407I 1246 TGTACA T^GTACA
BspDI 106 ATCGAT AT^CGAT
BspMI 378 GCAGGT ACCTGC(4/8)
BsrGI 1246 TGTACA T^GTACA
BstXI 1704 CCAAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1946 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 106 ATCGAT AT^CGAT
DraII 1119 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 213 CCATGG C^C[GA][CT]GG
EcoO109I 1119 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2206 GAATTC G^AATTC
EcoT14I 213 CCATGG C^C[AT][AT]GG
EcoT22I 2144 ATGCAT ATGCA^T
FauI 858 GCGGG CCCGC(4/6)
KpnI 369 GGTACC GGTAC^C
MamI 322 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1989 TGGCCA TGG^CCA
MscI 1989 TGGCCA TGG^CCA
NcoI 213 CCATGG C^CATGG
NsiI 2144 ATGCAT ATGCA^T
PflMI 1091 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1119 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1036 ATTAAT AT^TAAT
PstI 1454 CTGCAG CTGCA^G
SacI 1897 GAGCTC GAGCT^C
ScaI 1050 AGTACT AGT^ACT
SspBI 1246 TGTACA T^GTACA
StyI 213 CCATGG C^C[AT][AT]GG
Van91I 1091 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 43 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 454 GTCGAC GT^[AC][GT]AC
1324 GTCGAC
AflIII 426 ACATGT A^C[GA][CT]GT
2163 ACATGT
Asp700I 926 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAAGGGATTC
BanI 369 GGTACC G^G[CT][GA]CC
2261 GGCACC
BfaI 44 CTAG C^TAG
1373 CTAG
BpmI 527 CTGGAG CTGGAG(16/14)
830 CTGGAG
BsaJI 213 CCATGG C^C[ACGT][ACGT]GG
1181 CCAGGG
BseRI 478 GAGGAG GAGGAG(10/8)
1074 GAGGAG
BspLU11I 426 ACATGT A^CATGT
2163 ACATGT
EaeI 1487 TGGCCG [CT]^GGCC[GA]
1989 TGGCCA
EarI 301 GAAGAG CTCTTC(1/4)
926 GAAGAG
HincII 454 GTCGAC GT[CT]^[GA]AC
1324 GTCGAC
HindII 454 GTCGAC GT[CT]^[GA]AC
1324 GTCGAC
Ksp632I 301 GAAGAG CTCTTC(1/4)
926 GAAGAG
MaeI 44 CTAG C^TAG
1373 CTAG
NspI 426 ACATGT [GA]CATG^[CT]
2163 ACATGT
SalI 454 GTCGAC G^TCGAC
1324 GTCGAC
XmnI 926 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAAGGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BbsI BbvI
BclI BmyI BpuAI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI EcoRII EcoRV FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I