Restriction map for
pFN21AB9130
(Circular) (Six-base) MAPSORT of: pFN21AB9130.seq Check: 455 from: 1 to: 6988
pFN21AB9130 6988 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4798 278
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2460 3330 4331 1937
Size: 523 870 1001 4594
Fragments arranged by size:
4594 1001 870 523
Acc65I G'GTAC_C
Cuts at: 2374 4309 2374
Size: 1935 5053
AclI AA'CG_TT
Cuts at: 1563 5115 5488 1563
Size: 3552 373 3063
Fragments arranged by size:
3552 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2229 2295 2625 2748 2784 5051 6435
Size: 531 66 66 330 123 36 2267 1384
Cuts at: 6435 6703 1632
Size: 268 1917
Fragments arranged by size:
2267 1917 1384 531 330 268 123 66
66 36
AflII C'TTAA_G
Cuts at: 820 1017 3818 820
Size: 197 2801 3990
Fragments arranged by size:
3990 2801 197
AflIII A'CryG_T
Cuts at: 1203 2431 4168 5887 6864 1203
Size: 1228 1737 1719 977 1327
Fragments arranged by size:
1737 1719 1327 1228 977
AgeI A'CCGG_T
Cuts at: 5819 5819
Size: 6988
AhdI GACnn_n'nnGTC
Cuts at: 5717 5717
Size: 6988
AleI CACnn'nnGTG
Cuts at: 1166 3775 3864 4204 4206 1166
Size: 2609 89 340 2 3948
Fragments arranged by size:
3948 2609 340 89 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6988
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2421
Size: 32 6956
AlwNI CAG_nnn'CTG
Cuts at: 2141 2243 2516 2834 3957 4731 6303 2141
Size: 102 273 318 1123 774 1572 2826
Fragments arranged by size:
2826 1572 1123 774 318 273 102
ApaLI G'TGCA_C
Cuts at: 5045 6201 5045
Size: 1156 5832
ApoI r'AATT_y
Cuts at: 1493 3027 3344 3508 4211 4297 4523 4675 6784
Size: 1534 317 164 703 86 226 152 2109
Cuts at: 6784 1493
Size: 1697
Fragments arranged by size:
2109 1697 1534 703 317 226 164 152
86
AseI AT'TA_AT
Cuts at: 160 1032 3042 5542 160
Size: 872 2010 2500 1606
Fragments arranged by size:
2500 2010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
AvaI C'yCGr_G
Cuts at: 1870 1943 4313 1870
Size: 73 2370 4545
Fragments arranged by size:
4545 2370 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2460 2493 2460
Size: 33 6955
BamHI G'GATC_C
Cuts at: 4318 4685 4318
Size: 367 6621
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 4266 4309 5764 618
Size: 325 217 627 587 1892 43 1455 1842
Fragments arranged by size:
1892 1842 1455 627 587 325 217 43
BanII G_rGCy'C
Cuts at: 1391 3906 4307 1391
Size: 2515 401 4072
Fragments arranged by size:
4072 2515 401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 4097 4240 928
Size: 971 714 1484 143 3676
Fragments arranged by size:
3676 1484 971 714 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5179 5213 1903
Size: 34 3242 34 3678
Fragments arranged by size:
3678 3242 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4880 6090 4880
Size: 1210 5778
BclI T'GATC_A
Cuts at: 1548 2191 2332 3223 1548
Size: 643 141 891 5313
Fragments arranged by size:
5313 891 643 141
BfrBI ATG'CAT
Cuts at: 4151 4151
Size: 6988
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5599 136
Size: 107 122 71 631 605 3927 1525
Fragments arranged by size:
3927 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6983 6983
Size: 6988
BlpI GC'TnA_GC
Cuts at: 4405 4405
Size: 6988
Bme1580I G_kGCm'C
Cuts at: 1790 5049 6205 1790
Size: 3259 1156 2573
Fragments arranged by size:
3259 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4719 5677 442
Size: 416 53 83 399 24 3302 958 1753
Fragments arranged by size:
3302 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 2856 5648 1239
Size: 1314 303 2792 2579
Fragments arranged by size:
2792 2579 1314 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2886 3548 5113 5978 6276 2886
Size: 662 1565 865 298 3598
Fragments arranged by size:
3598 1565 865 662 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 5651 882
Size: 617 613 3539 2219
Fragments arranged by size:
3539 2219 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 4779 493
Size: 711 3575 2702
Fragments arranged by size:
3575 2702 711
BsaBI GATnn'nnATC
Cuts at: 2331 6982 2331
Size: 4651 2337
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4795 5177 5812 275
Size: 53 83 186 936 3262 382 635 1451
Fragments arranged by size:
3262 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5421 5819 6093 6240 6651 5421
Size: 398 274 147 411 5758
Fragments arranged by size:
5758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6763
Fragments arranged by size:
6763 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3094 1186
Size: 1312 596 5080
Fragments arranged by size:
5080 1312 596
BseYI C'CCAG_C
Cuts at: 6191 6687 6191
Size: 496 6492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 3651 3970 1163
Size: 573 352 1563 319 4181
Fragments arranged by size:
4181 1563 573 352 319
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4438 5199 6227 1540
Size: 275 186 2437 761 1028 2301
Fragments arranged by size:
2437 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2600 3803 3906 5049 5134 6205 2600
Size: 1203 103 1143 85 1071 3383
Fragments arranged by size:
3383 1203 1143 1071 103 85
BsmI GAATG_Cn'
Cuts at: 2862 4499 4592 2862
Size: 1637 93 5258
Fragments arranged by size:
5258 1637 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2600 3803 3906 4307 5049 5134 6205
Size: 399 810 1203 103 401 742 85 1071
Cuts at: 6205 1391
Size: 2174
Fragments arranged by size:
2174 1203 1071 810 742 401 399 103
85
BspEI T'CCGG_A
Cuts at: 6651 6651
Size: 6988
BspHI T'CATG_A
Cuts at: 4877 6607 4877
Size: 1730 5258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2374 4343 844
Size: 1530 1969 3489
Fragments arranged by size:
3489 1969 1530
BsrBI CCG'CTC
Cuts at: 1394 1823 4875 1394
Size: 429 3052 3507
Fragments arranged by size:
3507 3052 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5483 5657 65
Size: 1183 4235 174 1396
Fragments arranged by size:
4235 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5632 5819 1700
Size: 110 3822 187 2869
Fragments arranged by size:
3822 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3251 96
Size: 3155 3833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6988
BssSI C'ACGA_G
Cuts at: 5048 6060 5048
Size: 1012 5976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6988
BstBI TT'CG_AA
Cuts at: 5792 5792
Size: 6988
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6988
BstXI CCAn_nnnn'nTGG
Cuts at: 3716 3716
Size: 6988
BstYI r'GATC_y
Cuts at: 1972 4318 4685 5070 5087 6539 6625 6637 6983
Size: 2346 367 385 17 1452 86 12 346
Cuts at: 6983 1972
Size: 1977
Fragments arranged by size:
2346 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2218 513
Size: 552 939 214 5283
Fragments arranged by size:
5283 939 552 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6249
Fragments arranged by size:
6249 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3107 4504 5298 5318 2689
Size: 418 1397 794 20 4359
Fragments arranged by size:
4359 1397 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 2112 1554
Size: 558 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6953
DraI TTT'AAA
Cuts at: 2752 3181 3967 4162 4292 2752
Size: 429 786 195 130 5448
Fragments arranged by size:
5448 786 429 195 130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5995 809
Size: 507 4679 1802
Fragments arranged by size:
4679 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3492 3994 4435
Size: 54 1425 50 275 18 1662 502 441
Cuts at: 4435 5324 6953 8
Size: 889 1629 43
Fragments arranged by size:
1662 1629 1425 889 502 441 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4435 1537
Size: 275 2623 4090
Fragments arranged by size:
4090 2623 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 2926 4918 1353
Size: 948 625 1992 3423
Fragments arranged by size:
3423 1992 948 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5575 5949 6095 387
Size: 1064 138 3986 374 146 1280
Fragments arranged by size:
3986 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3904 3904
Size: 6988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2229 2295 2553 2625 2748 2784
Size: 393 531 66 66 258 72 123 36
Cuts at: 2784 2856 5051 5648 6435 6703 1239
Size: 72 2195 597 787 268 1524
Fragments arranged by size:
2195 1524 787 597 531 393 268 258
123 72 72 66 66 36
EcoO109I rG'GnC_Cy
Cuts at: 3125 3125
Size: 6988
EcoRI G'AATT_C
Cuts at: 4211 4297 4675 4211
Size: 86 378 6524
Fragments arranged by size:
6524 378 86
EcoRV GAT'ATC
Cuts at: 2230 2622 2917 3922 4176 2230
Size: 392 295 1005 254 5042
Fragments arranged by size:
5042 1005 392 295 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2300 4298 2300
Size: 1998 4990
FspI TGC'GCA
Cuts at: 5494 5494
Size: 6988
HaeII r_GCGC'y
Cuts at: 1678 6135 1678
Size: 4457 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 3126 3158 5629
Size: 32 135 32 642 32 880 32 2471
Cuts at: 5629 5661 5703 5735 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2471 880 642 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 3331 4332 4578 669
Size: 988 281 523 870 1001 246 3079
Fragments arranged by size:
3079 1001 988 870 523 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6988
HpaI GTT'AAC
Cuts at: 4578 4578
Size: 6988
KpnI G_GTAC'C
Cuts at: 2378 4313 2378
Size: 1935 5053
MfeI C'AATT_G
Cuts at: 4587 4587
Size: 6988
MluI A'CGCG_T
Cuts at: 6864 6864
Size: 6988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2617 6102 6286 1305
Size: 1312 3485 184 2007
Fragments arranged by size:
3485 2007 1312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3996 10
Size: 54 1425 343 2164 3002
Fragments arranged by size:
3002 2164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2112 3522 3588 3604 3691 3775
Size: 648 903 43 1410 66 16 87 84
Cuts at: 3775 3864 4033 4204 4206 4946 5305 5464 518
Size: 89 169 171 2 740 359 159 2042
Fragments arranged by size:
2042 1410 903 740 648 359 171 169
159 89 87 84 66 43 16 2
MspA1I CmG'CkG
Cuts at: 1606 2207 2531 2863 3113 4404 5081 6229 6474
Size: 601 324 332 250 1291 677 1148 245
Cuts at: 6474 1606
Size: 2120
Fragments arranged by size:
2120 1291 1148 677 601 332 324 250
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2218 513
Size: 552 939 214 5283
Fragments arranged by size:
5283 939 552 214
NdeI CA'TA_TG
Cuts at: 387 2655 3573 4086 387
Size: 2268 918 513 3289
Fragments arranged by size:
3289 2268 918 513
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6988
NotI GC'GGCC_GC
Cuts at: 4435 4435
Size: 6988
NsiI A_TGCA'T
Cuts at: 4153 4153
Size: 6988
NspI r_CATG'y
Cuts at: 1132 1229 2435 4172 4346 5891 1132
Size: 97 1206 1737 174 1545 2229
Fragments arranged by size:
2229 1737 1545 1206 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6988
PciI A'CATG_T
Cuts at: 2431 4168 5887 2431
Size: 1737 1719 3532
Fragments arranged by size:
3532 1737 1719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3102 1108
Size: 1994 4994
PmeI GTTT'AAAC
Cuts at: 4292 4292
Size: 6988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5057 5089 6570 6602 5057
Size: 32 1481 32 5443
Fragments arranged by size:
5443 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3125 3125
Size: 6988
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6988
PsiI TTA'TAA
Cuts at: 2735 3119 4558 2735
Size: 384 1439 5165
Fragments arranged by size:
5165 1439 384
PstI C_TGCA'G
Cuts at: 830 3463 4340 830
Size: 2633 877 3478
Fragments arranged by size:
3478 2633 877
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6988
PvuII CAG'CTG
Cuts at: 2207 2531 3113 2207
Size: 324 582 6082
Fragments arranged by size:
6082 582 324
SacI G_AGCT'C
Cuts at: 3906 3906
Size: 6988
SalI G'TCGA_C
Cuts at: 1936 2459 3329 4330 1936
Size: 523 870 1001 4594
Fragments arranged by size:
4594 1001 870 523
SbfI CC_TGCA'GG
Cuts at: 4340 4340
Size: 6988
ScaI AGT'ACT
Cuts at: 3057 5236 6948 3057
Size: 2179 1712 3097
Fragments arranged by size:
3097 2179 1712
SfcI C'TryA_G
Cuts at: 826 1045 2550 3459 4143 4336 5471 6152 6343
Size: 219 1505 909 684 193 1135 681 191
Cuts at: 6343 826
Size: 1471
Fragments arranged by size:
1505 1471 1135 909 684 681 219 193
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6988
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6988
SmaI CCC'GGG
Cuts at: 1872 4315 1872
Size: 2443 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2901 3563 3818 5092 5993 6255
Size: 197 926 958 662 255 1274 901 262
Cuts at: 6255 820
Size: 1553
Fragments arranged by size:
1553 1274 958 926 901 662 262 255
197
SnaBI TAC'GTA
Cuts at: 493 4779 493
Size: 4286 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6988
SphI G_CATG'C
Cuts at: 1132 4346 1132
Size: 3214 3774
SspI AAT'ATT
Cuts at: 5 52 4912 5
Size: 47 4860 2081
Fragments arranged by size:
4860 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6988
StyI C'CwwG_G
Cuts at: 513 1065 2004 2218 4427 513
Size: 552 939 214 2209 3074
Fragments arranged by size:
3074 2209 939 552 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5015 5032 5185 5370 5815 325
Size: 1255 3435 17 153 185 445 1498
Fragments arranged by size:
3435 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 3005 3055
Size: 274 80 33 51 1443 834 194 50
Cuts at: 3055 3251 5234 6946 96
Size: 196 1983 1712 138
Fragments arranged by size:
1983 1712 1443 834 274 196 194 138
80 51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6948 6980 6948
Size: 32 6956
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6988
XbaI T'CTAG_A
Cuts at: 2048 4324 2048
Size: 2276 4712
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6988
XmaI C'CCGG_G
Cuts at: 1870 4313 1870
Size: 2443 4545
XmnI GAAnn'nnTTC
Cuts at: 2935 4226 5117 2935
Size: 1291 891 4806
Fragments arranged by size:
4806 1291 891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4796 276
Size: 53 83 186 936 3262 2468
Fragments arranged by size:
3262 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI