(Circular) (Six-base) MAPSORT of: pFN21AE0528.seq Check: 5079 from: 1 to: 5569 pFN21AE0528 5569 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2235 2912 1937 Size: 298 677 4594 Fragments arranged by size: 4594 677 298 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 3632 5016 5284 1632 Size: 443 1557 1384 268 1917 Fragments arranged by size: 1917 1557 1384 443 268 AfeI AGC'GCT Cuts at: 2342 2342 Size: 5569 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2750 2760 1184 Size: 1566 10 3993 Fragments arranged by size: 3993 1566 10 AlwNI CAG_nnn'CTG Cuts at: 2281 2662 2849 3312 4884 2281 Size: 381 187 463 1572 2966 Fragments arranged by size: 2966 1572 463 381 187 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 5569 ApaLI G'TGCA_C Cuts at: 2075 3626 4782 2075 Size: 1551 1156 2862 Fragments arranged by size: 2862 1551 1156 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2381 2894 1870 Size: 73 190 248 513 4545 Fragments arranged by size: 4545 513 248 190 73 BamHI G'GATC_C Cuts at: 2899 3266 2899 Size: 367 5202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2701 2890 4345 618 Size: 325 217 627 914 189 1455 1842 Fragments arranged by size: 1842 1455 914 627 325 217 189 BanII G_rGCy'C Cuts at: 1391 2219 2301 2382 2693 2888 1391 Size: 828 82 81 311 195 4072 Fragments arranged by size: 4072 828 311 195 82 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 928 Size: 971 316 4282 Fragments arranged by size: 4282 971 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3760 3794 1903 Size: 34 1823 34 3678 Fragments arranged by size: 3678 1823 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2305 4180 136 Size: 107 122 71 631 605 633 1875 1525 Fragments arranged by size: 1875 1525 633 631 605 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2986 2986 Size: 5569 Bme1580I G_kGCm'C Cuts at: 1790 2079 2301 2704 3630 4786 1790 Size: 289 222 403 926 1156 2573 Fragments arranged by size: 2573 1156 926 403 289 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 3300 4258 Size: 416 53 83 399 24 796 1087 958 Cuts at: 4258 442 Size: 1753 Fragments arranged by size: 1753 1087 958 796 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2291 2591 4229 1239 Size: 968 84 300 1638 2579 Fragments arranged by size: 2579 1638 968 300 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3694 4559 4857 3694 Size: 865 298 4406 Fragments arranged by size: 4406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3376 3758 4393 275 Size: 53 83 186 936 1843 382 635 1451 Fragments arranged by size: 1843 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2774 2804 1178 Size: 30 165 30 1371 30 3943 Fragments arranged by size: 3943 1371 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2207 2663 1186 Size: 1018 3 456 4092 Fragments arranged by size: 4092 1018 456 3 BseYI C'CCAG_C Cuts at: 2477 4772 5268 2477 Size: 2295 496 2778 Fragments arranged by size: 2778 2295 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 1163 Size: 573 501 4495 Fragments arranged by size: 4495 573 501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2445 3630 3715 4786 2079 Size: 366 1185 85 1071 2862 Fragments arranged by size: 2862 1185 1071 366 85 BsmI GAATG_Cn' Cuts at: 2098 3080 3173 2098 Size: 982 93 4494 Fragments arranged by size: 4494 982 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2130 2130 Size: 5569 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2219 2301 2382 2445 2693 2704 Size: 399 289 140 82 81 63 248 11 Cuts at: 2704 2888 3630 3715 4786 1391 Size: 184 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 248 184 140 85 82 81 63 11 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 2103 3458 5188 2103 Size: 1355 1730 2484 Fragments arranged by size: 2484 1730 1355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 2924 844 Size: 1637 443 3489 Fragments arranged by size: 3489 1637 443 BsrBI CCG'CTC Cuts at: 1394 1823 2553 2790 3456 1394 Size: 429 730 237 666 3507 Fragments arranged by size: 3507 730 666 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 2517 4064 4238 65 Size: 1183 1269 1547 174 1396 Fragments arranged by size: 1547 1396 1269 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4213 4400 1700 Size: 110 2403 187 2869 Fragments arranged by size: 2869 2403 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 2360 3629 4641 2360 Size: 1269 1012 3288 Fragments arranged by size: 3288 1269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2093 2662 794 Size: 1299 569 3701 Fragments arranged by size: 3701 1299 569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2128 2420 1184 Size: 944 292 4333 Fragments arranged by size: 4333 944 292 BstYI r'GATC_y Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564 Size: 927 367 385 17 1452 86 12 346 Cuts at: 5564 1972 Size: 1977 Fragments arranged by size: 1977 1452 927 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2751 2865 513 Size: 552 939 747 114 3217 Fragments arranged by size: 3217 939 747 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4830 Fragments arranged by size: 4830 683 56 BtsI GCAGTG_nn' Cuts at: 3085 3879 3899 3085 Size: 794 20 4755 Fragments arranged by size: 4755 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DraIII CAC_nnn'GTG Cuts at: 2365 2365 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534 Size: 54 1425 50 275 18 1186 889 1629 Cuts at: 5534 8 Size: 43 Fragments arranged by size: 1629 1425 1186 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 2601 3499 1353 Size: 888 360 898 3423 Fragments arranged by size: 3423 898 888 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2207 2291 2591 3632 4229 5016 Size: 393 443 132 84 300 1041 597 787 Cuts at: 5016 5284 1239 Size: 268 1524 Fragments arranged by size: 1524 1041 787 597 443 393 300 268 132 84 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2189 2273 2057 Size: 132 84 5353 Fragments arranged by size: 5353 132 84 EcoO109I rG'GnC_Cy Cuts at: 2050 2278 2298 2491 2815 2050 Size: 228 20 193 324 4804 Fragments arranged by size: 4804 324 228 193 20 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2344 4716 1678 Size: 666 2372 2531 Fragments arranged by size: 2531 2372 666 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373 Size: 32 135 32 2638 32 42 32 2626 Fragments arranged by size: 2638 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4683 4867 1305 Size: 3378 184 2007 Fragments arranged by size: 3378 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3747 Fragments arranged by size: 3747 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2177 3527 3886 4045 518 Size: 648 1009 2 1350 359 159 2042 Fragments arranged by size: 2042 1350 1009 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2304 2719 2985 3662 4810 5055 1606 Size: 698 415 266 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 415 266 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2865 513 Size: 552 939 861 3217 Fragments arranged by size: 3217 939 861 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2032 2927 4472 1132 Size: 97 803 895 1545 2229 Fragments arranged by size: 2229 1545 895 803 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2365 1108 Size: 1257 4312 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 2815 2278 Size: 537 5032 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 5569 PstI C_TGCA'G Cuts at: 830 2921 830 Size: 2091 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SapI GCTCTTCn'nnn_ Cuts at: 2241 2241 Size: 5569 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2850 2917 4052 4733 4924 826 Size: 219 1805 67 1135 681 191 1471 Fragments arranged by size: 1805 1471 1135 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3673 4574 4836 820 Size: 197 926 1730 901 262 1553 Fragments arranged by size: 1730 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2032 2927 1132 Size: 900 895 3774 Fragments arranged by size: 3774 900 895 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2679 2865 3008 513 Size: 552 939 63 612 186 143 3074 Fragments arranged by size: 3074 939 612 552 186 143 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2408 3596 3613 3766 3951 4396 Size: 1255 516 312 1188 17 153 185 445 Cuts at: 4396 325 Size: 1498 Fragments arranged by size: 1498 1255 1188 516 445 312 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5529 5561 5529 Size: 32 5537 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI