################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BciVI BfrI BglII BlnI BsiWI BspDI BsrDI BssSI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoRI FseI FspI KpnI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SapI ScaI SexAI SgrAI SmaI SmlI SnaBI SpeI SspI StuI SwaI Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 3201 TCCGGA T^CCGGA AhdI 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 691 GTGCAC G^TGCAC ApaLI 691 GTGCAC G^TGCAC AspEI 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3097 CCCGAG C^[CT]CG[GA]G BbeI 21 GGCGCC GGCGC^C BbvCI 2644 GCTGAGG CCTCAGC(-5/-2) BcgI 1940 GCAGCGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 881 TGATCA T^GATCA BglI 2281 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2053 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2053 GCTGAGC GC^T[ACGT]AGC BsaAI 2967 CACGTA [CT]AC^GT[GA] BsaBI 2796 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3201 TCCGGA [AT]^CCGG[AT] BseAI 3201 TCCGGA T^CCGGA BsmI 2523 GAATGC GAATGC(1/-1) BsoBI 3097 CCCGAG C^[CT]CG[GA]G Bsp1407I 362 TGTACA T^GTACA BspEI 3201 TCCGGA T^CCGGA BspHI 2183 TCATGA T^CATGA BspLU11I 134 ACATGT A^CATGT BsrBI 2791 GAGCGG CCGCTC(-3/-3) BsrGI 362 TGTACA T^GTACA BssHII 30 GCGCGC G^CGCGC BstAPI 3128 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2870 TTCGAA TT^CGAA BstEII 1239 GGTCACC G^GT[ACGT]ACC BstPI 1239 GGTCACC G^GT[ACGT]ACC BstYI 1151 AGATCC [GA]^GATC[CT] CelII 2053 GCTGAGC GC^T[ACGT]AGC DraII 160 AGGGCCT [GA]G^G[ACGT]CC[CT] EagI 188 CGGCCG C^GGCCG Eam1105I 217 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 188 CGGCCG C^GGCCG Eco52I 188 CGGCCG C^GGCCG EcoNI 2196 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 160 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1239 GGTCACC G^GT[ACGT]ACC EcoRV 1561 GATATC GAT^ATC FbaI 881 TGATCA T^GATCA HpaI 2155 GTTAAC GTT^AAC KasI 21 GGCGCC G^GCGCC KspI 2020 CCGCGG CCGC^GG MamI 2796 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1151 AGATCC [GA]^GATC[CT] MroI 3201 TCCGGA T^CCGGA NarI 21 GGCGCC GG^CGCC NdeI 877 CATATG CA^TATG NspV 2870 TTCGAA TT^CGAA PflFI 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 2183 TCATGA T^CATGA SacII 2020 CCGCGG CCGC^GG SfiI 2280 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 21 GGCGCC GGC^GCC SfuI 2870 TTCGAA TT^CGAA SspBI 362 TGTACA T^GTACA TfiI 1003 GATTC G^A[AT]TC Tth111I 327 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1151 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 1794 GACGTC GACGT^C 3087 GACGTC AccI 1420 GTCGAC GT^[AC][GT]AC 2652 GTCGAC AflIII 134 ACATGT A^C[GA][CT]GT 2573 ACACGT AlwI 1152 GATCC GGATC(4/5) 2712 GATCC Aor51HI 658 AGCGCT AGC^GCT 755 AGCGCT BanI 21 GGCGCC G^G[CT][GA]CC 500 GGCACC BsgI 412 CTGCAC GTGCAG(16/14) 580 GTGCAG BsiEI 188 CGGCCG CG[GA][CT]^CG 2331 CGACCG BsmBI 703 GAGACG CGTCTC(1/5) 1279 GAGACG BsrFI 590 ACCGGC [GA]^CCGG[CT] 2058 GCCGGT BstXI 1101 CCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1958 CCATCAAGATGG Cfr10I 590 ACCGGC [GA]^CCGG[CT] 2058 GCCGGT Eco47III 658 AGCGCT AGC^GCT 755 AGCGCT EcoT22I 1121 ATGCAT ATGCA^T 2797 ATGCAT HindIII 968 AAGCTT A^AGCTT 1900 AAGCTT NcoI 512 CCATGG C^CATGG 1826 CCATGG NsiI 1121 ATGCAT ATGCA^T 2797 ATGCAT PshAI 1281 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1767 GACCCTGGTC PvuII 474 CAGCTG CAG^CTG 583 CAGCTG SalI 1420 GTCGAC G^TCGAC 2652 GTCGAC SbfI 858 CCTGCAGG CCTGCA^GG 1191 CCTGCAGG SphI 548 GCATGC GCATG^C 2243 GCATGC Sse8387I 858 CCTGCAGG CCTGCA^GG 1191 CCTGCAGG XcmI 242 CCAGTGACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1826 CCATGGAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I