(Circular) (Six-base) MAPSORT of: pFN21AE3581.seq Check: 612 from: 1 to: 7948 pFN21AE3581 7948 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2527 3388 2527 Size: 861 7087 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3803 5096 5758 278 Size: 53 83 186 936 2267 1293 662 2468 Fragments arranged by size: 2468 2267 1293 936 662 186 83 53 AccI GT'mk_AC Cuts at: 1937 3426 4658 5291 1937 Size: 1489 1232 633 4594 Fragments arranged by size: 4594 1489 1232 633 Acc65I G'GTAC_C Cuts at: 5269 5269 Size: 7948 AclI AA'CG_TT Cuts at: 1563 6075 6448 1563 Size: 4512 373 3063 Fragments arranged by size: 4512 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 2849 2939 3044 3182 4577 6011 7395 Size: 928 289 90 105 138 1395 1434 1384 Cuts at: 7395 7663 1632 Size: 268 1917 Fragments arranged by size: 1917 1434 1395 1384 928 289 268 138 105 90 AfeI AGC'GCT Cuts at: 2665 2762 2665 Size: 97 7851 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7751 AflIII A'CryG_T Cuts at: 1203 2139 4578 6847 7824 1203 Size: 936 2439 2269 977 1327 Fragments arranged by size: 2439 2269 1327 977 936 AgeI A'CCGG_T Cuts at: 6779 6779 Size: 7948 AhdI GACnn_n'nnGTC Cuts at: 2227 6677 2227 Size: 4450 3498 AleI CACnn'nnGTG Cuts at: 1166 2238 2333 2681 1166 Size: 1072 95 348 6433 Fragments arranged by size: 6433 1072 348 95 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7948 AlwNI CAG_nnn'CTG Cuts at: 2169 2481 3160 3775 4848 5691 7263 2169 Size: 312 679 615 1073 843 1572 2854 Fragments arranged by size: 2854 1572 1073 843 679 615 312 ApaLI G'TGCA_C Cuts at: 2696 6005 7161 2696 Size: 3309 1156 3483 Fragments arranged by size: 3483 3309 1156 ApoI r'AATT_y Cuts at: 1493 5257 5483 5635 7744 1493 Size: 3764 226 152 2109 1697 Fragments arranged by size: 3764 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6502 160 Size: 872 5470 1606 Fragments arranged by size: 5470 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 AvaI C'yCGr_G Cuts at: 1870 1943 5102 5273 1870 Size: 73 3159 171 4545 Fragments arranged by size: 4545 3159 171 73 BamHI G'GATC_C Cuts at: 5278 5645 5278 Size: 367 7581 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2505 5269 6724 618 Size: 325 217 627 239 479 2764 1455 1842 Fragments arranged by size: 2764 1842 1455 627 479 325 239 217 BanII G_rGCy'C Cuts at: 1391 2127 2160 2568 2994 3185 3207 3701 4052 Size: 736 33 408 426 191 22 494 351 Cuts at: 4052 4790 5267 1391 Size: 738 477 4072 Fragments arranged by size: 4072 738 736 494 477 426 408 351 191 33 22 BbeI G_GCGC'C Cuts at: 2030 2030 Size: 7948 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2331 2817 4921 5115 5217 928 Size: 971 432 486 2104 194 102 3659 Fragments arranged by size: 3659 2104 971 486 432 194 102 BbvCI CC'TCA_GC Cuts at: 4650 4650 Size: 7948 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3934 3968 6139 6173 1903 Size: 34 50 34 1913 34 2171 34 3678 Fragments arranged by size: 3678 2171 1913 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5840 7050 5840 Size: 1210 6738 BclI T'GATC_A Cuts at: 1548 2886 1548 Size: 1338 6610 BfrBI ATG'CAT Cuts at: 3128 4804 3128 Size: 1676 6272 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4292 6559 136 Size: 107 122 71 631 605 2620 2267 1525 Fragments arranged by size: 2620 2267 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7943 7943 Size: 7948 BlpI GC'TnA_GC Cuts at: 4059 5365 4059 Size: 1306 6642 Bme1580I G_kGCm'C Cuts at: 1790 2147 2700 3342 6009 7165 1790 Size: 357 553 642 2667 1156 2573 Fragments arranged by size: 2667 2573 1156 642 553 357 BmgBI CAC'GTC Cuts at: 1318 3363 4354 4437 4581 1318 Size: 2045 991 83 144 4685 Fragments arranged by size: 4685 2045 991 144 83 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4723 5679 6637 Size: 416 53 83 399 24 3306 956 958 Cuts at: 6637 442 Size: 1753 Fragments arranged by size: 3306 1753 958 956 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7948 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3769 3769 Size: 7948 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 3002 3008 4661 4706 4795 6608 1239 Size: 1130 633 6 1653 45 89 1813 2579 Fragments arranged by size: 2579 1813 1653 1130 633 89 45 6 Bpu10I CC'TnA_GC Cuts at: 3459 4650 4784 5183 3459 Size: 1191 134 399 6224 Fragments arranged by size: 6224 1191 399 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6073 6938 7236 6073 Size: 865 298 6785 Fragments arranged by size: 6785 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 2291 2732 3784 6611 882 Size: 617 738 54 441 1052 2827 2219 Fragments arranged by size: 2827 2219 1052 738 617 441 54 BsaAI yAC'GTr Cuts at: 493 1204 4974 5739 493 Size: 711 3770 765 2702 Fragments arranged by size: 3770 2702 765 711 BsaBI GATnn'nnATC Cuts at: 4805 7942 4805 Size: 3137 4811 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2814 3287 3800 Size: 53 83 186 936 494 787 473 513 Cuts at: 3800 4661 5093 5755 6137 6772 275 Size: 861 432 662 382 635 1451 Fragments arranged by size: 1451 936 861 787 662 635 513 494 473 432 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5206 6381 6779 7053 7200 7611 5206 Size: 1175 398 274 147 411 5543 Fragments arranged by size: 5543 1175 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2366 2396 2411 Size: 30 165 30 768 30 165 30 15 Cuts at: 2411 2441 1178 Size: 30 6685 Fragments arranged by size: 6685 768 165 165 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2117 2309 2419 3503 3851 3893 4447 Size: 840 91 192 110 1084 348 42 554 Cuts at: 4447 4658 4667 1186 Size: 211 9 4467 Fragments arranged by size: 4467 1084 840 554 348 211 192 110 91 42 9 BseYI C'CCAG_C Cuts at: 2174 2567 2996 3633 3647 3788 4016 4704 7151 Size: 393 429 637 14 141 228 688 2447 Cuts at: 7151 7647 2174 Size: 496 2475 Fragments arranged by size: 2475 2447 688 637 496 429 393 228 141 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2606 1163 Size: 573 666 204 6505 Fragments arranged by size: 6505 666 573 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 4339 5398 6159 7187 1540 Size: 275 186 195 2143 1059 761 1028 2301 Fragments arranged by size: 2301 2143 1059 1028 761 275 195 186 BsiHKAI G_wGCw'C Cuts at: 2160 2700 2994 3185 3207 6009 6094 7165 2160 Size: 540 294 191 22 2802 85 1071 2943 Fragments arranged by size: 2943 2802 1071 540 294 191 85 22 BsmI GAATG_Cn' Cuts at: 4534 5459 5552 4534 Size: 925 93 6930 Fragments arranged by size: 6930 925 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2702 3278 2702 Size: 576 7372 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2147 2160 2568 2700 2994 3185 Size: 399 337 20 13 408 132 294 191 Cuts at: 3185 3207 3342 3701 4052 4790 5267 6009 6094 Size: 22 135 359 351 738 477 742 85 Cuts at: 6094 7165 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 738 477 408 399 359 351 337 294 191 135 132 85 22 20 13 BspEI T'CCGG_A Cuts at: 5206 7611 5206 Size: 2405 5543 BspHI T'CATG_A Cuts at: 4188 5837 7567 4188 Size: 1649 1730 4569 Fragments arranged by size: 4569 1730 1649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2424 2527 3388 5236 5303 844 Size: 1580 103 861 1848 67 3489 Fragments arranged by size: 3489 1848 1580 861 103 67 BsrBI CCG'CTC Cuts at: 1394 1823 4798 5835 1394 Size: 429 2975 1037 3507 Fragments arranged by size: 3507 2975 1037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6443 6617 65 Size: 1183 5195 174 1396 Fragments arranged by size: 5195 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2595 4063 6592 6779 1700 Size: 110 785 1468 2529 187 2869 Fragments arranged by size: 2869 2529 1468 785 187 110 BsrGI T'GTAC_A Cuts at: 96 2367 96 Size: 2271 5677 BssHII G'CGCG_C Cuts at: 1925 2035 1925 Size: 110 7838 BssSI C'ACGA_G Cuts at: 6008 7020 6008 Size: 1012 6936 BstAPI GCAn_nnn'nTGC Cuts at: 794 5139 794 Size: 4345 3603 BstBI TT'CG_AA Cuts at: 4876 6752 4876 Size: 1876 6072 BstEII G'GTnAC_C Cuts at: 1184 3244 1184 Size: 2060 5888 BstXI CCAn_nnnn'nTGG Cuts at: 3113 3970 3113 Size: 857 7091 BstYI r'GATC_y Cuts at: 1972 3156 5278 5645 6030 6047 7499 7585 7597 Size: 1184 2122 367 385 17 1452 86 12 Cuts at: 7597 7943 1972 Size: 346 1977 Fragments arranged by size: 2122 1977 1452 1184 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2517 3831 4025 4464 513 Size: 552 1452 1314 194 439 3997 Fragments arranged by size: 3997 1452 1314 552 439 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3999 487 Size: 683 56 2773 4436 Fragments arranged by size: 4436 2773 683 56 BtsI GCAGTG_nn' Cuts at: 3207 5464 6258 6278 3207 Size: 2257 794 20 4877 Fragments arranged by size: 4877 2257 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7913 DraI TTT'AAA Cuts at: 5252 5252 Size: 7948 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6955 809 Size: 507 5639 1802 Fragments arranged by size: 5639 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2193 2845 Size: 54 1425 50 275 18 300 63 652 Cuts at: 2845 3103 3229 4122 4293 4854 4918 5395 6284 Size: 258 126 893 171 561 64 477 889 Cuts at: 6284 7913 8 Size: 1629 43 Fragments arranged by size: 1629 1425 893 889 652 561 477 300 275 258 171 126 64 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2193 5395 1537 Size: 275 381 3202 4090 Fragments arranged by size: 4090 3202 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2286 2579 3480 3511 3537 3704 4385 Size: 699 234 293 901 31 26 167 681 Cuts at: 4385 4667 5878 1353 Size: 282 1211 3423 Fragments arranged by size: 3423 1211 901 699 681 293 282 234 167 31 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4292 6535 6909 7055 387 Size: 1064 138 2703 2243 374 146 1280 Fragments arranged by size: 2703 2243 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2158 2992 3183 3205 2158 Size: 834 191 22 6901 Fragments arranged by size: 6901 834 191 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2560 2849 2939 3002 3008 3044 Size: 393 737 191 289 90 63 6 36 Cuts at: 3044 3182 4577 4661 4706 4795 6011 6608 7395 Size: 138 1395 84 45 89 1216 597 787 Cuts at: 7395 7663 1239 Size: 268 1524 Fragments arranged by size: 1524 1395 1216 787 737 597 393 289 268 191 138 90 89 84 63 45 36 6 EcoNI CCTnn'n_nnAGG Cuts at: 4205 4205 Size: 7948 EcoO109I rG'GnC_Cy Cuts at: 2166 2166 Size: 7948 EcoRI G'AATT_C Cuts at: 5257 5635 5257 Size: 378 7570 EcoRV GAT'ATC Cuts at: 3568 3568 Size: 7948 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3838 5234 3838 Size: 1396 6552 FspI TGC'GCA Cuts at: 6454 6454 Size: 7948 HaeII r_GCGC'y Cuts at: 1678 2030 2517 2667 2764 4176 7095 1678 Size: 352 487 150 97 1412 2919 2531 Fragments arranged by size: 2919 2531 1412 487 352 150 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 4934 4966 5035 Size: 32 135 32 641 32 2689 32 69 Cuts at: 5035 5067 5110 5142 6589 6621 6663 6695 1373 Size: 32 43 32 1447 32 42 32 2626 Fragments arranged by size: 2689 2626 1447 641 135 69 43 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 3427 4162 4357 4659 5292 Size: 988 281 753 736 735 195 302 633 Cuts at: 5292 5538 669 Size: 246 3079 Fragments arranged by size: 3079 988 753 736 735 633 302 281 246 195 HindIII A'AGCT_T Cuts at: 748 2973 3905 748 Size: 2225 932 4791 Fragments arranged by size: 4791 2225 932 HpaI GTT'AAC Cuts at: 4162 5538 4162 Size: 1376 6572 KasI G'GCGC_C Cuts at: 2026 2026 Size: 7948 KpnI G_GTAC'C Cuts at: 5273 5273 Size: 7948 MfeI C'AATT_G Cuts at: 5547 5547 Size: 7948 MluI A'CGCG_T Cuts at: 7824 7824 Size: 7948 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 2409 2424 3096 7062 7246 1305 Size: 1014 90 15 672 3966 184 2007 Fragments arranged by size: 3966 2007 1014 672 184 90 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2847 3231 4295 4856 4920 Size: 54 1425 343 1015 384 1064 561 64 Cuts at: 4920 10 Size: 3038 Fragments arranged by size: 3038 1425 1064 1015 561 384 343 64 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2333 2681 3111 3968 4247 5906 Size: 648 1072 95 348 430 857 279 1659 Cuts at: 5906 6265 6424 518 Size: 359 159 2042 Fragments arranged by size: 2042 1659 1072 857 648 430 359 348 279 159 95 MspA1I CmG'CkG Cuts at: 1606 2481 2490 2590 2649 4027 4289 5364 6041 Size: 875 9 100 59 1378 262 1075 677 Cuts at: 6041 7189 7434 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1378 1148 1075 875 677 262 245 100 59 9 NarI GG'CG_CC Cuts at: 2027 2027 Size: 7948 NcoI C'CATG_G Cuts at: 513 1065 2517 3831 513 Size: 552 1452 1314 4630 Fragments arranged by size: 4630 1452 1314 552 NdeI CA'TA_TG Cuts at: 387 2883 387 Size: 2496 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7948 NotI GC'GGCC_GC Cuts at: 5395 5395 Size: 7948 NsiI A_TGCA'T Cuts at: 3130 4806 3130 Size: 1676 6272 NspI r_CATG'y Cuts at: 1132 1229 2143 2557 2839 4252 5306 6851 1132 Size: 97 914 414 282 1413 1054 1545 2229 Fragments arranged by size: 2229 1545 1413 1054 914 414 282 97 PasI CC'CwG_GG Cuts at: 1348 3694 1348 Size: 2346 5602 PciI A'CATG_T Cuts at: 2139 6847 2139 Size: 4708 3240 PflMI CCAn_nnn'nTGG Cuts at: 1108 2218 2763 3220 3775 4852 1108 Size: 1110 545 457 555 1077 4204 Fragments arranged by size: 4204 1110 1077 555 545 457 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 7948 PmeI GTTT'AAAC Cuts at: 5252 5252 Size: 7948 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6017 6049 7530 7562 6017 Size: 32 1481 32 6403 Fragments arranged by size: 6403 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3290 3776 1613 Size: 1677 486 5785 Fragments arranged by size: 5785 1677 486 PsiI TTA'TAA Cuts at: 5518 5518 Size: 7948 PstI C_TGCA'G Cuts at: 830 2535 2663 2868 3201 4182 4314 5300 830 Size: 1705 128 205 333 981 132 986 3478 Fragments arranged by size: 3478 1705 986 981 333 205 132 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7948 PvuII CAG'CTG Cuts at: 2481 2590 2481 Size: 109 7839 SacI G_AGCT'C Cuts at: 2160 2994 3185 3207 2160 Size: 834 191 22 6901 Fragments arranged by size: 6901 834 191 22 SacII CC_GC'GG Cuts at: 4028 4028 Size: 7948 SalI G'TCGA_C Cuts at: 1936 3425 4657 5290 1936 Size: 1489 1232 633 4594 Fragments arranged by size: 4594 1489 1232 633 SbfI CC_TGCA'GG Cuts at: 2868 3201 5300 2868 Size: 333 2099 5516 Fragments arranged by size: 5516 2099 333 ScaI AGT'ACT Cuts at: 6196 7908 6196 Size: 1712 6236 SfcI C'TryA_G Cuts at: 826 1045 2531 2659 2864 3197 4178 4310 5296 Size: 219 1486 128 205 333 981 132 986 Cuts at: 5296 6431 7112 7303 826 Size: 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 986 981 681 333 219 205 191 132 128 SfiI GGCCn_nnn'nGGCC Cuts at: 4292 4292 Size: 7948 SfoI GGC'GCC Cuts at: 2028 2028 Size: 7948 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7948 SmaI CCC'GGG Cuts at: 1872 5275 1872 Size: 3403 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6052 6953 7215 820 Size: 197 926 4109 901 262 1553 Fragments arranged by size: 4109 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5739 493 Size: 5246 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7948 SphI G_CATG'C Cuts at: 1132 2557 4252 5306 1132 Size: 1425 1695 1054 3774 Fragments arranged by size: 3774 1695 1425 1054 SspI AAT'ATT Cuts at: 5 52 5872 5 Size: 47 5820 2081 Fragments arranged by size: 5820 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7948 StyI C'CwwG_G Cuts at: 513 1065 2301 2517 3552 3579 3831 4410 5387 Size: 552 1236 216 1035 27 252 579 977 Cuts at: 5387 513 Size: 3074 Fragments arranged by size: 3074 1236 1035 977 579 552 252 216 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2268 2756 3802 4023 4447 5975 5992 Size: 1255 688 488 1046 221 424 1528 17 Cuts at: 5992 6145 6330 6775 325 Size: 153 185 445 1498 Fragments arranged by size: 1528 1498 1255 1046 688 488 445 424 221 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2367 2838 Size: 274 80 33 51 1443 159 231 471 Cuts at: 2838 4767 6194 7906 96 Size: 1929 1427 1712 138 Fragments arranged by size: 1929 1712 1443 1427 471 274 231 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2411 2443 7908 7940 2411 Size: 32 5465 32 2419 Fragments arranged by size: 5465 2419 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2335 1316 Size: 1019 6929 XbaI T'CTAG_A Cuts at: 5284 5284 Size: 7948 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 3838 2254 Size: 1584 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7948 XmaI C'CCGG_G Cuts at: 1870 5273 1870 Size: 3403 4545 XmnI GAAnn'nnTTC Cuts at: 6077 6077 Size: 7948 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3801 5094 5756 276 Size: 53 83 186 936 2267 1293 662 2468 Fragments arranged by size: 2468 2267 1293 936 662 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI