(Linear) (Six-base) MAPSORT of: KIEE3581.seq Check: 3432 from: 1 to: 3243 KIEE3581.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 522 1383 3243 Size: 522 861 1860 Fragments arranged by size: 1860 861 522 AatII G_ACGT'C Cuts at: 0 1798 3091 3243 Size: 1798 1293 152 Fragments arranged by size: 1798 1293 152 AccI GT'mk_AC Cuts at: 0 1421 2653 3243 Size: 1421 1232 590 Fragments arranged by size: 1421 1232 590 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 555 844 934 1039 1177 2572 3243 Size: 555 289 90 105 138 1395 671 Fragments arranged by size: 1395 671 555 289 138 105 90 AfeI AGC'GCT Cuts at: 0 660 757 3243 Size: 660 97 2486 Fragments arranged by size: 2486 660 97 AflIII A'CryG_T Cuts at: 0 134 2573 3243 Size: 134 2439 670 Fragments arranged by size: 2439 670 134 AhdI GACnn_n'nnGTC Cuts at: 0 222 3243 Size: 222 3021 AleI CACnn'nnGTG Cuts at: 0 233 328 676 3243 Size: 233 95 348 2567 Fragments arranged by size: 2567 348 233 95 AlwNI CAG_nnn'CTG Cuts at: 0 164 476 1155 1770 2843 3243 Size: 164 312 679 615 1073 400 Fragments arranged by size: 1073 679 615 400 312 164 ApaLI G'TGCA_C Cuts at: 0 691 3243 Size: 691 2552 AvaI C'yCGr_G Cuts at: 0 3097 3243 Size: 3097 146 BanI G'GyrC_C Cuts at: 0 21 500 3243 Size: 21 479 2743 Fragments arranged by size: 2743 479 21 BanII G_rGCy'C Cuts at: 0 122 155 563 989 1180 1202 1696 2047 Size: 122 33 408 426 191 22 494 351 Cuts at: 2047 2785 3243 Size: 738 458 Fragments arranged by size: 738 494 458 426 408 351 191 122 33 22 BbeI G_GCGC'C Cuts at: 0 25 3243 Size: 25 3218 BbsI GAAGACnn'nnnn_ Cuts at: 0 326 812 2916 3110 3212 3243 Size: 326 486 2104 194 102 31 Fragments arranged by size: 2104 486 326 194 102 31 BbvCI CC'TCA_GC Cuts at: 0 2645 3243 Size: 2645 598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1929 1963 3243 Size: 1929 34 1280 Fragments arranged by size: 1929 1280 34 BclI T'GATC_A Cuts at: 0 881 3243 Size: 881 2362 BfrBI ATG'CAT Cuts at: 0 1123 2799 3243 Size: 1123 1676 444 Fragments arranged by size: 1676 1123 444 BglI GCCn_nnn'nGGC Cuts at: 0 2287 3243 Size: 2287 956 BlpI GC'TnA_GC Cuts at: 0 2054 3243 Size: 2054 1189 Bme1580I G_kGCm'C Cuts at: 0 142 695 1337 3243 Size: 142 553 642 1906 Fragments arranged by size: 1906 642 553 142 BmgBI CAC'GTC Cuts at: 0 1358 2349 2432 2576 3243 Size: 1358 991 83 144 667 Fragments arranged by size: 1358 991 667 144 83 BmrI ACTGGGnnnn_n' Cuts at: 0 2718 3243 Size: 2718 525 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1764 3243 Size: 1764 1479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 997 1003 2656 2701 2790 3243 Size: 364 633 6 1653 45 89 453 Fragments arranged by size: 1653 633 453 364 89 45 6 Bpu10I CC'TnA_GC Cuts at: 0 1454 2645 2779 3178 3243 Size: 1454 1191 134 399 65 Fragments arranged by size: 1454 1191 399 134 65 BsaI GGTCTCn'nnnn_ Cuts at: 0 232 286 727 1779 3243 Size: 232 54 441 1052 1464 Fragments arranged by size: 1464 1052 441 232 54 BsaAI yAC'GTr Cuts at: 0 2969 3243 Size: 2969 274 BsaBI GATnn'nnATC Cuts at: 0 2800 3243 Size: 2800 443 BsaHI Gr'CG_yC Cuts at: 0 22 809 1282 1795 2656 3088 3243 Size: 22 787 473 513 861 432 155 Fragments arranged by size: 861 787 513 473 432 155 22 BsaWI w'CCGG_w Cuts at: 0 3201 3243 Size: 3201 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 166 196 361 391 406 436 3243 Size: 166 30 165 30 15 30 2807 Fragments arranged by size: 2807 166 165 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 112 304 414 1498 1846 1888 2442 Size: 21 91 192 110 1084 348 42 554 Cuts at: 2442 2653 2662 3243 Size: 211 9 581 Fragments arranged by size: 1084 581 554 348 211 192 110 91 42 21 9 BseYI C'CCAG_C Cuts at: 0 169 562 991 1628 1642 1783 2011 2699 Size: 169 393 429 637 14 141 228 688 Cuts at: 2699 3243 Size: 544 Fragments arranged by size: 688 637 544 429 393 228 169 141 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 601 3243 Size: 397 204 2642 Fragments arranged by size: 2642 397 204 BsiEI CG_ry'CG Cuts at: 0 191 2334 3243 Size: 191 2143 909 Fragments arranged by size: 2143 909 191 BsiHKAI G_wGCw'C Cuts at: 0 155 695 989 1180 1202 3243 Size: 155 540 294 191 22 2041 Fragments arranged by size: 2041 540 294 191 155 22 BsmI GAATG_Cn' Cuts at: 0 2529 3243 Size: 2529 714 BsmBI CGTCTCn'nnnn_ Cuts at: 0 697 1273 3243 Size: 697 576 1970 Fragments arranged by size: 1970 697 576 Bsp1286I G_dGCh'C Cuts at: 0 122 142 155 563 695 989 1180 1202 Size: 122 20 13 408 132 294 191 22 Cuts at: 1202 1337 1696 2047 2785 3243 Size: 135 359 351 738 458 Fragments arranged by size: 738 458 408 359 351 294 191 135 132 122 22 20 13 BspEI T'CCGG_A Cuts at: 0 3201 3243 Size: 3201 42 BspHI T'CATG_A Cuts at: 0 2183 3243 Size: 2183 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 419 522 1383 3243 Size: 419 103 861 1860 Fragments arranged by size: 1860 861 419 103 BsrBI CCG'CTC Cuts at: 0 2793 3243 Size: 2793 450 BsrFI r'CCGG_y Cuts at: 0 590 2058 3243 Size: 590 1468 1185 Fragments arranged by size: 1468 1185 590 BsrGI T'GTAC_A Cuts at: 0 362 3243 Size: 362 2881 BssHII G'CGCG_C Cuts at: 0 30 3243 Size: 30 3213 BstAPI GCAn_nnn'nTGC Cuts at: 0 3134 3243 Size: 3134 109 BstBI TT'CG_AA Cuts at: 0 2871 3243 Size: 2871 372 BstEII G'GTnAC_C Cuts at: 0 1239 3243 Size: 1239 2004 BstXI CCAn_nnnn'nTGG Cuts at: 0 1108 1965 3243 Size: 1108 857 1278 Fragments arranged by size: 1278 1108 857 BstYI r'GATC_y Cuts at: 0 1151 3243 Size: 1151 2092 BtgI C'CryG_G Cuts at: 0 512 1826 2020 2459 3243 Size: 512 1314 194 439 784 Fragments arranged by size: 1314 784 512 439 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1994 3243 Size: 1994 1249 BtsI GCAGTG_nn' Cuts at: 0 1202 3243 Size: 1202 2041 EaeI y'GGCC_r Cuts at: 0 125 188 840 1098 1224 2117 2288 2849 Size: 125 63 652 258 126 893 171 561 Cuts at: 2849 2913 3243 Size: 64 330 Fragments arranged by size: 893 652 561 330 258 171 126 125 64 63 EagI C'GGCC_G Cuts at: 0 188 3243 Size: 188 3055 EarI CTCTTCn'nnn_ Cuts at: 0 47 281 574 1475 1506 1532 1699 2380 Size: 47 234 293 901 31 26 167 681 Cuts at: 2380 2662 3243 Size: 282 581 Fragments arranged by size: 901 681 581 293 282 234 167 47 31 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2287 3243 Size: 2287 956 EcoICRI GAG'CTC Cuts at: 0 153 987 1178 1200 3243 Size: 153 834 191 22 2043 Fragments arranged by size: 2043 834 191 153 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 555 844 934 997 1003 1039 1177 Size: 364 191 289 90 63 6 36 138 Cuts at: 1177 2572 2656 2701 2790 3243 Size: 1395 84 45 89 453 Fragments arranged by size: 1395 453 364 289 191 138 90 89 84 63 45 36 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 2200 3243 Size: 2200 1043 EcoO109I rG'GnC_Cy Cuts at: 0 161 3243 Size: 161 3082 EcoRV GAT'ATC Cuts at: 0 1563 3243 Size: 1563 1680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1833 3229 3243 Size: 1833 1396 14 Fragments arranged by size: 1833 1396 14 HaeII r_GCGC'y Cuts at: 0 25 512 662 759 2171 3243 Size: 25 487 150 97 1412 1072 Fragments arranged by size: 1412 1072 487 150 97 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 208 240 2929 2961 3030 3062 3105 3137 Size: 208 32 2689 32 69 32 43 32 Cuts at: 3137 3243 Size: 106 Fragments arranged by size: 2689 208 106 69 43 32 32 32 32 HincII GTy'rAC Cuts at: 0 686 1422 2157 2352 2654 3243 Size: 686 736 735 195 302 589 Fragments arranged by size: 736 735 686 589 302 195 HindIII A'AGCT_T Cuts at: 0 968 1900 3243 Size: 968 932 1343 Fragments arranged by size: 1343 968 932 HpaI GTT'AAC Cuts at: 0 2157 3243 Size: 2157 1086 KasI G'GCGC_C Cuts at: 0 21 3243 Size: 21 3222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 314 404 419 1091 3243 Size: 314 90 15 672 2152 Fragments arranged by size: 2152 672 314 90 15 MscI TGG'CCA Cuts at: 0 842 1226 2290 2851 2915 3243 Size: 842 384 1064 561 64 328 Fragments arranged by size: 1064 842 561 384 328 64 MslI CAynn'nnrTG Cuts at: 0 233 328 676 1106 1963 2242 3243 Size: 233 95 348 430 857 279 1001 Fragments arranged by size: 1001 857 430 348 279 233 95 MspA1I CmG'CkG Cuts at: 0 476 485 585 644 2022 2284 3243 Size: 476 9 100 59 1378 262 959 Fragments arranged by size: 1378 959 476 262 100 59 9 NarI GG'CG_CC Cuts at: 0 22 3243 Size: 22 3221 NcoI C'CATG_G Cuts at: 0 512 1826 3243 Size: 512 1314 1417 Fragments arranged by size: 1417 1314 512 NdeI CA'TA_TG Cuts at: 0 878 3243 Size: 878 2365 NsiI A_TGCA'T Cuts at: 0 1125 2801 3243 Size: 1125 1676 442 Fragments arranged by size: 1676 1125 442 NspI r_CATG'y Cuts at: 0 138 552 834 2247 3243 Size: 138 414 282 1413 996 Fragments arranged by size: 1413 996 414 282 138 PasI CC'CwG_GG Cuts at: 0 1689 3243 Size: 1689 1554 PciI A'CATG_T Cuts at: 0 134 3243 Size: 134 3109 PflMI CCAn_nnn'nTGG Cuts at: 0 213 758 1215 1770 2847 3243 Size: 213 545 457 555 1077 396 Fragments arranged by size: 1077 555 545 457 396 213 PfoI T'CCnGG_A Cuts at: 0 554 3243 Size: 554 2689 PshAI GACnn'nnGTC Cuts at: 0 1285 1771 3243 Size: 1285 486 1472 Fragments arranged by size: 1472 1285 486 PstI C_TGCA'G Cuts at: 0 530 658 863 1196 2177 2309 3243 Size: 530 128 205 333 981 132 934 Fragments arranged by size: 981 934 530 333 205 132 128 PvuII CAG'CTG Cuts at: 0 476 585 3243 Size: 476 109 2658 Fragments arranged by size: 2658 476 109 SacI G_AGCT'C Cuts at: 0 155 989 1180 1202 3243 Size: 155 834 191 22 2041 Fragments arranged by size: 2041 834 191 155 22 SacII CC_GC'GG Cuts at: 0 2023 3243 Size: 2023 1220 SalI G'TCGA_C Cuts at: 0 1420 2652 3243 Size: 1420 1232 591 Fragments arranged by size: 1420 1232 591 SbfI CC_TGCA'GG Cuts at: 0 863 1196 3243 Size: 863 333 2047 Fragments arranged by size: 2047 863 333 SfcI C'TryA_G Cuts at: 0 526 654 859 1192 2173 2305 3243 Size: 526 128 205 333 981 132 938 Fragments arranged by size: 981 938 526 333 205 132 128 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2287 3243 Size: 2287 956 SfoI GGC'GCC Cuts at: 0 23 3243 Size: 23 3220 SphI G_CATG'C Cuts at: 0 552 2247 3243 Size: 552 1695 996 Fragments arranged by size: 1695 996 552 StyI C'CwwG_G Cuts at: 0 296 512 1547 1574 1826 2405 3243 Size: 296 216 1035 27 252 579 838 Fragments arranged by size: 1035 838 579 296 252 216 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 263 751 1797 2018 2442 3243 Size: 263 488 1046 221 424 801 Fragments arranged by size: 1046 801 488 424 263 221 TatI w'GTAC_w Cuts at: 0 131 362 833 2762 3243 Size: 131 231 471 1929 481 Fragments arranged by size: 1929 481 471 231 131 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 406 438 3243 Size: 406 32 2805 Fragments arranged by size: 2805 406 32 Tth111I GACn'n_nGTC Cuts at: 0 330 3243 Size: 330 2913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 249 1833 3243 Size: 249 1584 1410 Fragments arranged by size: 1584 1410 249 ZraI GAC'GTC Cuts at: 0 1796 3089 3243 Size: 1796 1293 154 Fragments arranged by size: 1796 1293 154 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AleI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PshAI PstI PvuII SacI SacII SalI SbfI SfcI SfiI SfoI SphI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BglII BmtI BpuEI BsiWI BsrDI BssSI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoRI FseI FspI FspAI KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SapI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI