(Circular) (Six-base) MAPSORT of: pFN21AE0214.seq Check: 2719 from: 1 to: 6088 pFN21AE0214 6088 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2264 3431 1937 Size: 327 1167 4594 Fragments arranged by size: 4594 1167 327 Acc65I G'GTAC_C Cuts at: 3149 3243 3409 3149 Size: 94 166 5828 Fragments arranged by size: 5828 166 94 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3363 4151 5535 5803 1632 Size: 1731 788 1384 268 1917 Fragments arranged by size: 1917 1731 1384 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 2063 4919 2063 Size: 2856 3232 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 3089 1166 Size: 1923 4165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 3233 3831 5403 3233 Size: 598 1572 3918 Fragments arranged by size: 3918 1572 598 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2306 3219 3397 3623 3775 5884 1493 Size: 813 913 178 226 152 2109 1697 Fragments arranged by size: 2109 1697 913 813 226 178 152 AseI AT'TA_AT Cuts at: 160 1032 2246 4642 160 Size: 872 1214 2396 1606 Fragments arranged by size: 2396 1606 1214 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 AvrII C'CTAG_G Cuts at: 2941 2941 Size: 6088 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3070 3149 3243 3409 4864 Size: 325 217 627 1283 79 94 166 1455 Cuts at: 4864 618 Size: 1842 Fragments arranged by size: 1842 1455 1283 627 325 217 166 94 79 BanII G_rGCy'C Cuts at: 1391 3407 1391 Size: 2016 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 928 Size: 971 417 4700 Fragments arranged by size: 4700 971 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 2290 2473 1548 Size: 742 183 5163 Fragments arranged by size: 5163 742 183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 4149 5305 1790 Size: 2359 1156 2573 Fragments arranged by size: 2573 2359 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4748 1239 Size: 3509 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2063 4521 4919 5193 5340 5751 2063 Size: 2458 398 274 147 411 2400 Fragments arranged by size: 2458 2400 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2652 2682 1178 Size: 30 165 30 1249 30 4584 Fragments arranged by size: 4584 1249 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3204 1186 Size: 2018 4070 BseYI C'CCAG_C Cuts at: 5291 5787 5291 Size: 496 5592 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2338 4149 4234 5305 2338 Size: 1811 85 1071 3121 Fragments arranged by size: 3121 1811 1071 85 BsmI GAATG_Cn' Cuts at: 2183 2761 3599 3692 2183 Size: 578 838 93 4579 Fragments arranged by size: 4579 838 578 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2764 2764 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 3407 4149 4234 5305 1391 Size: 399 548 1069 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 742 548 399 85 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2035 3443 844 Size: 1191 1408 3489 Fragments arranged by size: 3489 1408 1191 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2832 4583 4757 65 Size: 1183 1584 1751 174 1396 Fragments arranged by size: 1751 1584 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 4732 4919 1700 Size: 110 253 2669 187 2869 Fragments arranged by size: 2869 2669 253 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 2506 4892 2506 Size: 2386 3702 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 2877 2877 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2662 3418 3785 4170 4187 5639 5725 5737 Size: 690 756 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 756 690 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 3195 3604 4398 4418 3195 Size: 409 794 20 4865 Fragments arranged by size: 4865 794 409 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4018 1353 Size: 2665 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3363 4151 4748 5535 5803 1239 Size: 393 1731 788 597 787 268 1524 Fragments arranged by size: 1731 1524 788 787 597 393 268 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 5235 1678 Size: 3557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2666 2698 2766 2798 4729 Size: 32 135 32 1094 32 68 32 1931 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1931 1094 135 68 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3153 3247 3413 3153 Size: 94 166 5828 Fragments arranged by size: 5828 166 94 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 4046 4405 4564 518 Size: 648 1923 957 359 159 2042 Fragments arranged by size: 2042 1923 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3324 3504 4181 5329 5574 1606 Size: 1718 180 677 1148 245 2120 Fragments arranged by size: 2120 1718 1148 677 245 180 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 2982 387 Size: 2595 3493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2746 3446 4991 1132 Size: 97 1517 700 1545 2229 Fragments arranged by size: 2229 1545 1517 700 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2352 2445 1108 Size: 1244 93 4751 Fragments arranged by size: 4751 1244 93 PfoI T'CCnGG_A Cuts at: 2187 2187 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3336 3368 3336 Size: 32 6056 PstI C_TGCA'G Cuts at: 830 2229 3202 3440 830 Size: 1399 973 238 3478 Fragments arranged by size: 3478 1399 973 238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 3324 3324 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2225 3198 3436 4571 5252 5443 826 Size: 219 1180 973 238 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 973 681 238 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 2933 152 Size: 2781 3307 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 2143 4012 5 Size: 47 2091 1869 2081 Fragments arranged by size: 2091 2081 1869 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2941 3527 513 Size: 552 1876 586 3074 Fragments arranged by size: 3074 1876 586 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4115 4132 4285 4470 4915 325 Size: 1255 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3041 4334 6046 Size: 274 80 33 51 1443 1064 1293 1712 Cuts at: 6046 96 Size: 138 Fragments arranged by size: 1712 1443 1293 1064 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2652 2684 6048 6080 2652 Size: 32 3364 32 2660 Fragments arranged by size: 3364 2660 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2976 4217 2976 Size: 1241 4847 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI