Restriction map for
pFN21AE6706
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspEI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI MamI MluI
MluNI MroI MscI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PvuI SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AclI 527 AACGTT AA^CGTT
AlwI 2475 GGATC GGATC(4/5)
BsaAI 263 TACGTG [CT]AC^GT[GA]
BseRI 1213 GAGGAG GAGGAG(10/8)
BspDI 2016 ATCGAT AT^CGAT
BsrDI 2459 CATTGC GCAATG(2/0)
BstAPI 39 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1607 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2016 ATCGAT AT^CGAT
CpoI 65 CGGACCG CG^G[AT]CCG
DraI 1321 TTTAAA TTT^AAA
DrdI 607 GACTTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 427 CCTGTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 505 GAATTC G^AATTC
FseI 138 GGCCGGCC GGCCGG^CC
HgaI 91 GACGC GACGC(5/10)
KspI 160 CCGCGG CCGC^GG
MslI 2077 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2515 CCATGG C^CATGG
NdeI 2762 CATATG CA^TATG
PflMI 2944 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1217 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 527 AACGTT AA^CGTT
PstI 1171 CTGCAG CTGCA^G
RsrII 65 CGGACCG CG^G[AT]CCG
SacII 160 CCGCGG CCGC^GG
ScaI 1128 AGTACT AGT^ACT
SmlI 736 CTTGAG C^T[CT][GA]AG
Van91I 2944 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2036 CCAAGGCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AgeI 68 ACCGGT A^CCGGT
967 ACCGGT
BanI 45 GGTGCC G^G[CT][GA]CC
1667 GGCACC
BbsI 1376 GAAGAC GAAGAC(2/6)
2653 GAAGAC
BbvCI 2323 CCTCAGC CCTCAGC(-5/-2)
1444 GCTGAGG
BcnI 186 CCGGG CC^[GC]GG
2745 CCCGG
BfaI 2356 CTAG C^TAG
2914 CTAG
BglII 2064 AGATCT A^GATCT
2571 AGATCT
BlpI 1909 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
Bpu1102I 1909 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
BpuAI 1376 GAAGAC GAAGAC(2/6)
2653 GAAGAC
BsaI 365 GAGACC GGTCTC(1/5)
437 GAGACC
BsmI 1553 GCATTC GAATGC(1/-1)
1704 GCATTC
BspHI 2772 TCATGA T^CATGA
2930 TCATGA
BssSI 305 CTCGTG CACGAG(-5/-1)
1208 CTCGTG
CelII 1909 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
DsaI 160 CCGCGG C^C[GA][CT]GG
2515 CCATGG
EaeI 137 TGGCCG [CT]^GGCC[GA]
141 CGGCCA
EarI 238 GAAGAG CTCTTC(1/4)
2785 GAAGAG
Ksp632I 238 GAAGAG CTCTTC(1/4)
2785 GAAGAG
MaeI 2356 CTAG C^TAG
2914 CTAG
MfeI 233 CAATTG C^AATTG
2897 CAATTG
MunI 233 CAATTG C^AATTG
2897 CAATTG
NaeI 139 GCCGGC GCC^GGC
2665 GCCGGC
NciI 186 CCGGG CC^[GC]GG
2745 CCCGG
NgoMIV 139 GCCGGC G^CCGGC
2665 GCCGGC
NspI 2072 ACATGC [GA]CATG^[CT]
2362 GCATGT
PinAI 68 ACCGGT A^CCGGT
967 ACCGGT
RcaI 2772 TCATGA T^CATGA
2930 TCATGA
SspI 854 AATATT AAT^ATT
1957 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI AseI AsnI
Asp700I AvaII BbvI BciVI BmyI
BpmI BsaJI BsaWI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI