(Circular) (Six-base) MAPSORT of: pFN21AE6706.seq Check: 4031 from: 1 to: 7717 pFN21AE6706 7717 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2035 2886 2035 Size: 851 6866 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5527 278 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 3102 3516 4341 5060 1937 Size: 894 271 414 825 719 4594 Fragments arranged by size: 4594 894 825 719 414 271 Acc65I G'GTAC_C Cuts at: 5038 5038 Size: 7717 AclI AA'CG_TT Cuts at: 1563 2533 5844 6217 1563 Size: 970 3311 373 3063 Fragments arranged by size: 3311 3063 970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2350 3266 3384 3780 4218 4373 4946 5780 Size: 718 916 118 396 438 155 573 834 Cuts at: 5780 7164 7432 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 916 834 718 573 438 396 268 155 118 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7520 AflIII A'CryG_T Cuts at: 1203 6616 7593 1203 Size: 5413 977 1327 Fragments arranged by size: 5413 1327 977 AgeI A'CCGG_T Cuts at: 2073 2972 6548 2073 Size: 899 3576 3242 Fragments arranged by size: 3576 3242 899 AhdI GACnn_n'nnGTC Cuts at: 6446 6446 Size: 7717 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7717 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2649 2681 4161 4193 2649 Size: 32 1480 32 6173 Fragments arranged by size: 6173 1480 32 32 AlwNI CAG_nnn'CTG Cuts at: 2155 2878 3362 5460 7032 2155 Size: 723 484 2098 1572 2840 Fragments arranged by size: 2840 2098 1572 723 484 ApaLI G'TGCA_C Cuts at: 5774 6930 5774 Size: 1156 6561 ApoI r'AATT_y Cuts at: 1493 2510 2730 2998 3463 5026 5252 5404 7513 Size: 1017 220 268 465 1563 226 152 2109 Cuts at: 7513 1493 Size: 1697 Fragments arranged by size: 2109 1697 1563 1017 465 268 226 220 152 AseI AT'TA_AT Cuts at: 160 1032 2502 3821 3834 3966 6271 160 Size: 872 1470 1319 13 132 2305 1606 Fragments arranged by size: 2305 1606 1470 1319 872 132 13 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 AvaI C'yCGr_G Cuts at: 1870 1943 5042 1870 Size: 73 3099 4545 Fragments arranged by size: 4545 3099 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3408 3441 3408 Size: 33 7684 BamHI G'GATC_C Cuts at: 5047 5414 5047 Size: 367 7350 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 3672 5038 6493 618 Size: 325 217 627 263 1622 1366 1455 1842 Fragments arranged by size: 1842 1622 1455 1366 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 5036 1391 Size: 3645 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3388 4665 928 Size: 971 1489 1277 3980 Fragments arranged by size: 3980 1489 1277 971 BbvCI CC'TCA_GC Cuts at: 3450 4329 3450 Size: 879 6838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5908 5942 1903 Size: 34 3971 34 3678 Fragments arranged by size: 3971 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2330 3120 3997 5609 6819 2330 Size: 790 877 1612 1210 3228 Fragments arranged by size: 3228 1612 1210 877 790 BclI T'GATC_A Cuts at: 1548 1548 Size: 7717 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6328 136 Size: 107 122 71 631 605 4656 1525 Fragments arranged by size: 4656 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4069 4576 7712 4069 Size: 507 3136 4074 Fragments arranged by size: 4074 3136 507 BlpI GC'TnA_GC Cuts at: 3915 4356 5134 3915 Size: 441 778 6498 Fragments arranged by size: 6498 778 441 Bme1580I G_kGCm'C Cuts at: 1790 2055 3675 3933 5778 6934 1790 Size: 265 1620 258 1845 1156 2573 Fragments arranged by size: 2573 1845 1620 1156 265 258 BmgBI CAC'GTC Cuts at: 1318 3157 4531 1318 Size: 1839 1374 4504 Fragments arranged by size: 4504 1839 1374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5448 6406 442 Size: 416 53 83 399 24 4031 958 1753 Fragments arranged by size: 4031 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2465 2530 4464 2465 Size: 65 1934 5718 Fragments arranged by size: 5718 1934 65 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2241 2305 2657 2846 2933 3648 4447 Size: 845 157 64 352 189 87 715 799 Cuts at: 4447 6377 1239 Size: 1930 2579 Fragments arranged by size: 2579 1930 845 799 715 352 189 157 87 64 Bpu10I CC'TnA_GC Cuts at: 3450 4308 4329 3450 Size: 858 21 6838 Fragments arranged by size: 6838 858 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 5842 6707 7005 2762 Size: 3080 865 298 3474 Fragments arranged by size: 3474 3080 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2364 2436 6380 882 Size: 617 865 72 3944 2219 Fragments arranged by size: 3944 2219 865 617 72 BsaAI yAC'GTr Cuts at: 493 1204 2270 5508 493 Size: 711 1066 3238 2702 Fragments arranged by size: 3238 2702 1066 711 BsaBI GATnn'nnATC Cuts at: 7711 7711 Size: 7717 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5524 5906 6541 275 Size: 53 83 186 936 3991 382 635 1451 Fragments arranged by size: 3991 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2073 2972 4797 6150 6548 6822 6969 7380 2073 Size: 899 1825 1353 398 274 147 411 2410 Fragments arranged by size: 2410 1825 1353 899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2649 2679 2931 2961 3212 Size: 30 165 30 1246 30 252 30 251 Cuts at: 3212 3242 1178 Size: 30 5653 Fragments arranged by size: 5653 1246 252 251 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3233 1186 Size: 2047 5670 BseYI C'CCAG_C Cuts at: 2185 3455 3705 6920 7416 2185 Size: 1270 250 3215 496 2486 Fragments arranged by size: 3215 2486 1270 496 250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2009 2030 2443 3217 1163 Size: 573 273 21 413 774 5663 Fragments arranged by size: 5663 774 573 413 273 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5167 5928 6956 1540 Size: 275 186 3166 761 1028 2301 Fragments arranged by size: 3166 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5778 5863 6934 5778 Size: 85 1071 6561 Fragments arranged by size: 6561 1071 85 BsmI GAATG_Cn' Cuts at: 3558 3709 5228 5321 3558 Size: 151 1519 93 5954 Fragments arranged by size: 5954 1519 151 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 3675 3933 5036 5778 5863 6934 Size: 399 265 1620 258 1103 742 85 1071 Cuts at: 6934 1391 Size: 2174 Fragments arranged by size: 2174 1620 1103 1071 742 399 265 258 85 BspEI T'CCGG_A Cuts at: 7380 7380 Size: 7717 BspHI T'CATG_A Cuts at: 4777 4935 5606 7336 4777 Size: 158 671 1730 5158 Fragments arranged by size: 5158 1730 671 158 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 2035 2886 3183 5072 844 Size: 1187 4 851 297 1889 3489 Fragments arranged by size: 3489 1889 1187 851 297 4 BsrBI CCG'CTC Cuts at: 1394 1823 5604 1394 Size: 429 3781 3507 Fragments arranged by size: 3781 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4463 6212 6386 65 Size: 1183 3215 1749 174 1396 Fragments arranged by size: 3215 1749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2073 2144 2972 4670 6361 6548 1700 Size: 110 263 71 828 1698 1691 187 2869 Fragments arranged by size: 2869 1698 1691 828 263 187 110 71 BsrGI T'GTAC_A Cuts at: 96 2016 2379 3501 96 Size: 1920 363 1122 4312 Fragments arranged by size: 4312 1920 1122 363 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7717 BssSI C'ACGA_G Cuts at: 2310 3213 5777 6789 2310 Size: 903 2564 1012 3238 Fragments arranged by size: 3238 2564 1012 903 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 6461 BstBI TT'CG_AA Cuts at: 6521 6521 Size: 7717 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7717 BstXI CCAn_nnnn'nTGG Cuts at: 3619 3619 Size: 7717 BstYI r'GATC_y Cuts at: 1972 4069 4480 4576 5047 5414 5799 5816 7268 Size: 2097 411 96 471 367 385 17 1452 Cuts at: 7268 7354 7366 7712 1972 Size: 86 12 346 1977 Fragments arranged by size: 2097 1977 1452 471 411 385 367 346 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2165 4520 513 Size: 552 939 161 2355 3710 Fragments arranged by size: 3710 2355 939 552 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6978 Fragments arranged by size: 6978 683 56 BtsI GCAGTG_nn' Cuts at: 3185 3205 4056 5233 6027 6047 3185 Size: 20 851 1177 794 20 4855 Fragments arranged by size: 4855 1177 851 794 20 20 ClaI AT'CG_AT Cuts at: 1554 4022 1554 Size: 2468 5249 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3132 3167 529 Size: 35 2568 35 5079 Fragments arranged by size: 5079 2568 35 35 DraI TTT'AAA Cuts at: 3328 5021 3328 Size: 1693 6024 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2618 6724 809 Size: 507 1302 4106 1802 Fragments arranged by size: 4106 1802 1302 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2146 5164 Size: 54 1425 50 275 18 312 4 3018 Cuts at: 5164 6053 7682 8 Size: 889 1629 43 Fragments arranged by size: 3018 1629 1425 889 312 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 5164 1537 Size: 275 3352 4090 Fragments arranged by size: 4090 3352 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 4785 5647 1353 Size: 885 2547 862 3423 Fragments arranged by size: 3423 2547 885 862 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6304 6678 6824 387 Size: 1064 138 4715 374 146 1280 Fragments arranged by size: 4715 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2241 2305 2350 2657 2846 2933 Size: 393 452 157 64 45 307 189 87 Cuts at: 2933 3266 3384 3648 3780 4218 4373 4447 4946 Size: 333 118 264 132 438 155 74 499 Cuts at: 4946 5780 6377 7164 7432 1239 Size: 834 597 787 268 1524 Fragments arranged by size: 1524 834 787 597 499 452 438 393 333 307 268 264 189 157 155 132 118 87 74 64 45 EcoNI CCTnn'n_nnAGG Cuts at: 2436 2436 Size: 7717 EcoO109I rG'GnC_Cy Cuts at: 2407 2645 3223 2407 Size: 238 578 6901 Fragments arranged by size: 6901 578 238 EcoRI G'AATT_C Cuts at: 2510 5026 5404 2510 Size: 2516 378 4823 Fragments arranged by size: 4823 2516 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2462 2462 Size: 7717 FseI GG_CCGG'CC Cuts at: 2148 2148 Size: 7717 FspI TGC'GCA Cuts at: 6223 6223 Size: 7717 HaeII r_GCGC'y Cuts at: 1678 6864 1678 Size: 5186 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2677 2709 2894 2926 2962 Size: 32 135 32 1105 32 185 32 36 Cuts at: 2962 2994 3807 3839 6358 6390 6432 6464 1373 Size: 32 813 32 2519 32 42 32 2626 Fragments arranged by size: 2626 2519 1105 813 185 135 42 36 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5061 5307 669 Size: 988 281 3123 246 3079 Fragments arranged by size: 3123 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7717 HpaI GTT'AAC Cuts at: 5307 5307 Size: 7717 KpnI G_GTAC'C Cuts at: 5042 5042 Size: 7717 MfeI C'AATT_G Cuts at: 2238 4902 5316 2238 Size: 2664 414 4639 Fragments arranged by size: 4639 2664 414 MluI A'CGCG_T Cuts at: 7593 7593 Size: 7717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 4205 6831 7015 1305 Size: 1264 1636 2626 184 2007 Fragments arranged by size: 2626 2007 1636 1264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5895 Fragments arranged by size: 5895 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4086 5675 6034 6193 518 Size: 648 2920 1589 359 159 2042 Fragments arranged by size: 2920 2042 1589 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2167 2953 3619 5133 5810 6958 7203 Size: 408 153 786 666 1514 677 1148 245 Cuts at: 7203 1606 Size: 2120 Fragments arranged by size: 2120 1514 1148 786 677 666 408 245 153 NaeI GCC'GGC Cuts at: 2146 4672 2146 Size: 2526 5191 NcoI C'CATG_G Cuts at: 513 1065 2004 4520 513 Size: 552 939 2516 3710 Fragments arranged by size: 3710 2516 939 552 NdeI CA'TA_TG Cuts at: 387 4768 387 Size: 4381 3336 NgoMIV G'CCGG_C Cuts at: 2144 4670 2144 Size: 2526 5191 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7717 NotI GC'GGCC_GC Cuts at: 5164 5164 Size: 7717 NspI r_CATG'y Cuts at: 1132 1229 4081 4371 5075 6620 1132 Size: 97 2852 290 704 1545 2229 Fragments arranged by size: 2852 2229 1545 704 290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7717 PciI A'CATG_T Cuts at: 6616 6616 Size: 7717 PflMI CCAn_nnn'nTGG Cuts at: 1108 4955 1108 Size: 3847 3870 PmeI GTTT'AAAC Cuts at: 5021 5021 Size: 7717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2649 2681 5786 5818 7299 7331 2649 Size: 32 3105 32 1481 32 3035 Fragments arranged by size: 3105 3035 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3223 3223 Size: 7717 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7717 PsiI TTA'TAA Cuts at: 5287 5287 Size: 7717 PstI C_TGCA'G Cuts at: 830 3180 5069 830 Size: 2350 1889 3478 Fragments arranged by size: 3478 2350 1889 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7717 PvuII CAG'CTG Cuts at: 2014 2953 3619 2014 Size: 939 666 6112 Fragments arranged by size: 6112 939 666 RsrII CG'GwC_CG Cuts at: 2071 2071 Size: 7717 SacII CC_GC'GG Cuts at: 2168 2168 Size: 7717 SalI G'TCGA_C Cuts at: 1936 5059 1936 Size: 3123 4594 SbfI CC_TGCA'GG Cuts at: 5069 5069 Size: 7717 ScaI AGT'ACT Cuts at: 3135 5965 7677 3135 Size: 2830 1712 3175 Fragments arranged by size: 3175 2830 1712 SfcI C'TryA_G Cuts at: 826 1045 2209 2884 3176 3444 3520 3943 4495 Size: 219 1164 675 292 268 76 423 552 Cuts at: 4495 5065 6200 6881 7072 826 Size: 570 1135 681 191 1471 Fragments arranged by size: 1471 1164 1135 681 675 570 552 423 292 268 219 191 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7717 SmaI CCC'GGG Cuts at: 1872 5044 1872 Size: 3172 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2741 5821 6722 6984 820 Size: 197 926 798 3080 901 262 1553 Fragments arranged by size: 3080 1553 926 901 798 262 197 SnaBI TAC'GTA Cuts at: 493 5508 493 Size: 5015 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7717 SphI G_CATG'C Cuts at: 1132 5075 1132 Size: 3943 3774 SspI AAT'ATT Cuts at: 5 52 2861 3964 5641 5 Size: 47 2809 1103 1677 2081 Fragments arranged by size: 2809 2081 1677 1103 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7717 StyI C'CwwG_G Cuts at: 513 1065 2004 3676 4041 4520 5156 513 Size: 552 939 1672 365 479 636 3074 Fragments arranged by size: 3074 1672 939 636 552 479 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3406 3447 5744 5761 5914 6099 6544 Size: 1255 1826 41 2297 17 153 185 445 Cuts at: 6544 325 Size: 1498 Fragments arranged by size: 2297 1826 1498 1255 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 2379 3133 Size: 274 80 33 51 1443 39 363 754 Cuts at: 3133 3501 4419 5963 7675 96 Size: 368 918 1544 1712 138 Fragments arranged by size: 1712 1544 1443 918 754 368 363 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4212 4244 7677 7709 4212 Size: 32 3433 32 4220 Fragments arranged by size: 4220 3433 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7717 XbaI T'CTAG_A Cuts at: 5053 5053 Size: 7717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4048 4048 Size: 7717 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7717 XmaI C'CCGG_G Cuts at: 1870 5042 1870 Size: 3172 4545 XmnI GAAnn'nnTTC Cuts at: 2860 3369 4931 5846 2860 Size: 509 1562 915 4731 Fragments arranged by size: 4731 1562 915 509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5525 276 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI