(Linear) (Six-base) MAPSORT of: KIEE6706.seq Check: 4957 from: 1 to: 3012 KIEE6706.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 30 881 3012 Size: 30 851 2131 Fragments arranged by size: 2131 851 30 AccI GT'mk_AC Cuts at: 0 826 1097 1511 2336 3012 Size: 826 271 414 825 676 Fragments arranged by size: 826 825 676 414 271 AclI AA'CG_TT Cuts at: 0 528 3012 Size: 528 2484 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 1261 1379 1775 2213 2368 2941 3012 Size: 345 916 118 396 438 155 573 71 Fragments arranged by size: 916 573 438 396 345 155 118 71 AgeI A'CCGG_T Cuts at: 0 68 967 3012 Size: 68 899 2045 Fragments arranged by size: 2045 899 68 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 644 676 2156 2188 3012 Size: 644 32 1480 32 824 Fragments arranged by size: 1480 824 644 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 150 873 1357 3012 Size: 150 723 484 1655 Fragments arranged by size: 1655 723 484 150 ApoI r'AATT_y Cuts at: 0 505 725 993 1458 3012 Size: 505 220 268 465 1554 Fragments arranged by size: 1554 505 465 268 220 AseI AT'TA_AT Cuts at: 0 497 1816 1829 1961 3012 Size: 497 1319 13 132 1051 Fragments arranged by size: 1319 1051 497 132 13 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1403 1436 3012 Size: 1403 33 1576 Fragments arranged by size: 1576 1403 33 BanI G'GyrC_C Cuts at: 0 45 1667 3012 Size: 45 1622 1345 Fragments arranged by size: 1622 1345 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 1383 2660 3012 Size: 1383 1277 352 Fragments arranged by size: 1383 1277 352 BbvCI CC'TCA_GC Cuts at: 0 1445 2324 3012 Size: 1445 879 688 Fragments arranged by size: 1445 879 688 BciVI GTATCCnnnnn_n' Cuts at: 0 325 1115 1992 3012 Size: 325 790 877 1020 Fragments arranged by size: 1020 877 790 325 BglII A'GATC_T Cuts at: 0 2064 2571 3012 Size: 2064 507 441 Fragments arranged by size: 2064 507 441 BlpI GC'TnA_GC Cuts at: 0 1910 2351 3012 Size: 1910 441 661 Fragments arranged by size: 1910 661 441 Bme1580I G_kGCm'C Cuts at: 0 50 1670 1928 3012 Size: 50 1620 258 1084 Fragments arranged by size: 1620 1084 258 50 BmgBI CAC'GTC Cuts at: 0 1152 2526 3012 Size: 1152 1374 486 Fragments arranged by size: 1374 1152 486 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 460 525 2459 3012 Size: 460 65 1934 553 Fragments arranged by size: 1934 553 460 65 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 236 300 652 841 928 1643 2442 Size: 79 157 64 352 189 87 715 799 Cuts at: 2442 3012 Size: 570 Fragments arranged by size: 799 715 570 352 189 157 87 79 64 Bpu10I CC'TnA_GC Cuts at: 0 1445 2303 2324 3012 Size: 1445 858 21 688 Fragments arranged by size: 1445 858 688 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 3012 Size: 757 2255 BsaI GGTCTCn'nnnn_ Cuts at: 0 359 431 3012 Size: 359 72 2581 Fragments arranged by size: 2581 359 72 BsaAI yAC'GTr Cuts at: 0 265 3012 Size: 265 2747 BsaWI w'CCGG_w Cuts at: 0 68 967 2792 3012 Size: 68 899 1825 220 Fragments arranged by size: 1825 899 220 68 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 644 674 926 956 1207 1237 3012 Size: 644 30 252 30 251 30 1775 Fragments arranged by size: 1775 644 252 251 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1228 3012 Size: 1228 1784 BseYI C'CCAG_C Cuts at: 0 180 1450 1700 3012 Size: 180 1270 250 1312 Fragments arranged by size: 1312 1270 250 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 25 438 1212 3012 Size: 4 21 413 774 1800 Fragments arranged by size: 1800 774 413 21 4 BsmI GAATG_Cn' Cuts at: 0 1553 1704 3012 Size: 1553 151 1308 Fragments arranged by size: 1553 1308 151 Bsp1286I G_dGCh'C Cuts at: 0 50 1670 1928 3012 Size: 50 1620 258 1084 Fragments arranged by size: 1620 1084 258 50 BspHI T'CATG_A Cuts at: 0 2772 2930 3012 Size: 2772 158 82 Fragments arranged by size: 2772 158 82 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 26 30 881 1178 3012 Size: 26 4 851 297 1834 Fragments arranged by size: 1834 851 297 26 4 BsrDI GCAATG_nn' Cuts at: 0 2458 3012 Size: 2458 554 BsrFI r'CCGG_y Cuts at: 0 68 139 967 2665 3012 Size: 68 71 828 1698 347 Fragments arranged by size: 1698 828 347 71 68 BsrGI T'GTAC_A Cuts at: 0 11 374 1496 3012 Size: 11 363 1122 1516 Fragments arranged by size: 1516 1122 363 11 BssSI C'ACGA_G Cuts at: 0 305 1208 3012 Size: 305 903 1804 Fragments arranged by size: 1804 903 305 BstAPI GCAn_nnn'nTGC Cuts at: 0 45 3012 Size: 45 2967 BstXI CCAn_nnnn'nTGG Cuts at: 0 1614 3012 Size: 1614 1398 BstYI r'GATC_y Cuts at: 0 2064 2475 2571 3012 Size: 2064 411 96 441 Fragments arranged by size: 2064 441 411 96 BtgI C'CryG_G Cuts at: 0 160 2515 3012 Size: 160 2355 497 Fragments arranged by size: 2355 497 160 BtsI GCAGTG_nn' Cuts at: 0 1180 1200 2051 3012 Size: 1180 20 851 961 Fragments arranged by size: 1180 961 851 20 ClaI AT'CG_AT Cuts at: 0 2017 3012 Size: 2017 995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1127 1162 3012 Size: 1127 35 1850 Fragments arranged by size: 1850 1127 35 DraI TTT'AAA Cuts at: 0 1323 3012 Size: 1323 1689 DrdI GACnn_nn'nnGTC Cuts at: 0 613 3012 Size: 613 2399 EaeI y'GGCC_r Cuts at: 0 137 141 3012 Size: 137 4 2871 Fragments arranged by size: 2871 137 4 EarI CTCTTCn'nnn_ Cuts at: 0 233 2780 3012 Size: 233 2547 232 Fragments arranged by size: 2547 233 232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 236 300 345 652 841 928 1261 Size: 79 157 64 45 307 189 87 333 Cuts at: 1261 1379 1643 1775 2213 2368 2442 2941 3012 Size: 118 264 132 438 155 74 499 71 Fragments arranged by size: 499 438 333 307 264 189 157 155 132 118 87 79 74 71 64 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 431 3012 Size: 431 2581 EcoO109I rG'GnC_Cy Cuts at: 0 402 640 1218 3012 Size: 402 238 578 1794 Fragments arranged by size: 1794 578 402 238 EcoRI G'AATT_C Cuts at: 0 505 3012 Size: 505 2507 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 457 3012 Size: 457 2555 FseI GG_CCGG'CC Cuts at: 0 143 3012 Size: 143 2869 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 672 704 889 921 957 989 1802 1834 Size: 672 32 185 32 36 32 813 32 Cuts at: 1834 3012 Size: 1178 Fragments arranged by size: 1178 813 672 185 36 32 32 32 32 MfeI C'AATT_G Cuts at: 0 233 2897 3012 Size: 233 2664 115 Fragments arranged by size: 2664 233 115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 564 2200 3012 Size: 564 1636 812 Fragments arranged by size: 1636 812 564 MslI CAynn'nnrTG Cuts at: 0 2081 3012 Size: 2081 931 MspA1I CmG'CkG Cuts at: 0 9 162 948 1614 3012 Size: 9 153 786 666 1398 Fragments arranged by size: 1398 786 666 153 9 NaeI GCC'GGC Cuts at: 0 141 2667 3012 Size: 141 2526 345 Fragments arranged by size: 2526 345 141 NcoI C'CATG_G Cuts at: 0 2515 3012 Size: 2515 497 NdeI CA'TA_TG Cuts at: 0 2763 3012 Size: 2763 249 NgoMIV G'CCGG_C Cuts at: 0 139 2665 3012 Size: 139 2526 347 Fragments arranged by size: 2526 347 139 NspI r_CATG'y Cuts at: 0 2076 2366 3012 Size: 2076 290 646 Fragments arranged by size: 2076 646 290 PflMI CCAn_nnn'nTGG Cuts at: 0 2950 3012 Size: 2950 62 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 644 676 3012 Size: 644 32 2336 Fragments arranged by size: 2336 644 32 PpuMI rG'GwC_Cy Cuts at: 0 1218 3012 Size: 1218 1794 PstI C_TGCA'G Cuts at: 0 1175 3012 Size: 1175 1837 PvuII CAG'CTG Cuts at: 0 9 948 1614 3012 Size: 9 939 666 1398 Fragments arranged by size: 1398 939 666 9 RsrII CG'GwC_CG Cuts at: 0 66 3012 Size: 66 2946 SacII CC_GC'GG Cuts at: 0 163 3012 Size: 163 2849 ScaI AGT'ACT Cuts at: 0 1130 3012 Size: 1130 1882 SfcI C'TryA_G Cuts at: 0 204 879 1171 1439 1515 1938 2490 3012 Size: 204 675 292 268 76 423 552 522 Fragments arranged by size: 675 552 522 423 292 268 204 76 SmlI C'TyrA_G Cuts at: 0 736 3012 Size: 736 2276 SspI AAT'ATT Cuts at: 0 856 1959 3012 Size: 856 1103 1053 Fragments arranged by size: 1103 1053 856 StyI C'CwwG_G Cuts at: 0 1671 2036 2515 3012 Size: 1671 365 479 497 Fragments arranged by size: 1671 497 479 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1401 1442 3012 Size: 1401 41 1570 Fragments arranged by size: 1570 1401 41 TatI w'GTAC_w Cuts at: 0 11 374 1128 1496 2414 3012 Size: 11 363 754 368 918 598 Fragments arranged by size: 918 754 598 368 363 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2207 2239 3012 Size: 2207 32 773 Fragments arranged by size: 2207 773 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2043 3012 Size: 2043 969 XmnI GAAnn'nnTTC Cuts at: 0 855 1364 2926 3012 Size: 855 509 1562 86 Fragments arranged by size: 1562 855 509 86 Enzymes that do cut: AarI AccI AclI AcuI AgeI AloI AlwNI ApoI AseI BaeI BanI BbsI BbvCI BciVI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI FseI Hin4I MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PflMI PpiI PpuMI PstI PvuII RsrII SacII ScaI SfcI SmlI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanII BbeI BcgI BclI BfrBI BglI BmrI BmtI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI DraIII EagI EciI EcoICRI EcoRV FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MscI NarI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI SacI SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI