################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbsI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 193 GCGG CCGC(-3/-1) AcsI 418 GAATTT [GA]^AATT[CT] AcyI 67 GGCGCC G[GA]^CG[CT]C Alw44I 136 GTGCAC G^TGCAC AlwI 11 GATCC GGATC(4/5) ApaLI 136 GTGCAC G^TGCAC ApoI 418 GAATTT [GA]^AATT[CT] AspHI 136 GTGCAC G[AT]GC[AT]^C BbeI 67 GGCGCC GGCGC^C BbvCI 76 GCTGAGG CCTCAGC(-5/-2) BciVI 400 GGATAC GTATCC(6/5) BcnI 106 CCCGG CC^[GC]GG BfaI 166 CTAG C^TAG BsaHI 67 GGCGCC G[GA]^CG[CT]C BseRI 79 GAGGAG GAGGAG(10/8) BsiHKAI 136 GTGCAC G[AT]GC[AT]^C BstYI 10 AGATCC [GA]^GATC[CT] CfoI 68 GCGC GCG^C DpnII 11 GATC ^GATC EcoT22I 235 ATGCAT ATGCA^T HaeII 67 GGCGCC [GA]GCGC^[CT] HapII 107 CCGG C^CGG HhaI 68 GCGC GCG^C Hin1I 67 GGCGCC G[GA]^CG[CT]C HinP1I 68 GCGC G^CGC HpaII 107 CCGG C^CGG KasI 67 GGCGCC G^GCGCC MaeI 166 CTAG C^TAG MboI 11 GATC ^GATC MboII 340 GAAGA GAAGA(8/7) MflI 10 AGATCC [GA]^GATC[CT] MseI 353 TTAA T^TAA MslI 238 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 107 CCGG C^CGG NarI 67 GGCGCC GG^CGCC NciI 106 CCCGG CC^[GC]GG NdeII 11 GATC ^GATC NsiI 235 ATGCAT ATGCA^T Sau3AI 11 GATC ^GATC SfaNI 162 GCATC GCATC(5/9) SfoI 67 GGCGCC GGC^GCC Tru9I 353 TTAA T^TAA XbaI 165 TCTAGA T^CTAGA XhoII 10 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 94 AGCT AG^CT 218 AGCT BanI 67 GGCGCC G^G[CT][GA]CC 324 GGCACC BmyI 59 GTGCCC G[AGT]GC[ACT]^C 136 GTGCAC BsaJI 63 CCCAGG C^C[ACGT][ACGT]GG 71 CCAGGG BsiYI 42 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 300 CCATAAGGAGG BslI 42 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 300 CCATAAGGAGG Bsp1286I 59 GTGCCC G[AGT]GC[ACT]^C 136 GTGCAC Cfr13I 50 GGGCC G^G[ACGT]CC 117 GGGCC PstI 86 CTGCAG CTGCA^G 433 CTGCAG Sau96I 50 GGGCC G^G[ACGT]CC 117 GGGCC SfcI 86 CTGCAG C^T[GA][CT]AG 433 CTGCAG TspRI 134 CAGTG CA[GC]TG[ACGT][ACGT]^ 431 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### BbvI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIII NlaIV ScrFI TseI Tsp509I