################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HinfI HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TspRI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AcsI 780 GAATTT [GA]^AATT[CT] AflIII 1138 ACGTGT A^C[GA][CT]GT AlwNI 910 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 12 GGGCCC GGGCC^C ApoI 780 GAATTT [GA]^AATT[CT] BbrPI 1137 CACGTG CAC^GTG BbvCI 1395 CCTCAGC CCTCAGC(-5/-2) BglI 1443 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmBI 1344 CGTCTC CGTCTC(1/5) BstYI 681 AGATCC [GA]^GATC[CT] EcoT14I 1169 CCAAGG C^C[AT][AT]GG MflI 681 AGATCC [GA]^GATC[CT] NheI 30 GCTAGC G^CTAGC NspI 356 ACATGC [GA]CATG^[CT] PmaCI 1137 CACGTG CAC^GTG PmlI 1137 CACGTG CAC^GTG PstI 136 CTGCAG CTGCA^G SacI 313 GAGCTC GAGCT^C SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G StyI 1169 CCAAGG C^C[AT][AT]GG TaqI 404 TCGA T^CGA TthHB8I 404 TCGA T^CGA XbaI 678 TCTAGA T^CTAGA XhoII 681 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 367 GTCTAC GT^[AC][GT]AC 1411 GTCTAC AfaI 435 GTAC GT^AC 864 GTAC AspHI 223 GTGCTC G[AT]GC[AT]^C 313 GAGCTC BanII 12 GGGCCC G[GA]GC[CT]^C 313 GAGCTC BbeI 36 GGCGCC GGCGC^C 105 GGCGCC BclI 629 TGATCA T^GATCA 800 TGATCA BfaI 31 CTAG C^TAG 679 CTAG BsaAI 865 TACGTG [CT]AC^GT[GA] 1137 CACGTG BsiHKAI 223 GTGCTC G[AT]GC[AT]^C 313 GAGCTC BsrBI 475 CCGCTC CCGCTC(-3/-3) 1276 GAGCGG BsrFI 39 GCCGGC [GA]^CCGG[CT] 108 GCCGGC BsrI 711 ACTGG ACTGG(1/-1) 1211 ACTGG BssSI 517 CTCGTG CACGAG(-5/-1) 1129 CTCGTG Cfr10I 39 GCCGGC [GA]^CCGG[CT] 108 GCCGGC DdeI 1268 CTGAG C^T[ACGT]AG 1396 CTCAG DraII 1021 GGGTCCC [GA]G^G[ACGT]CC[CT] 1057 GGGTCCC EagI 44 CGGCCG C^GGCCG 548 CGGCCG EclXI 44 CGGCCG C^GGCCG 548 CGGCCG Eco52I 44 CGGCCG C^GGCCG 548 CGGCCG EcoO109I 1021 GGGTCCC [GA]G^G[ACGT]CC[CT] 1057 GGGTCCC FbaI 629 TGATCA T^GATCA 800 TGATCA KasI 36 GGCGCC G^GCGCC 105 GGCGCC MaeI 31 CTAG C^TAG 679 CTAG MaeII 866 ACGT A^CGT 1138 ACGT NaeI 39 GCCGGC GCC^GGC 108 GCCGGC NarI 36 GGCGCC GG^CGCC 105 GGCGCC NgoMIV 39 GCCGGC G^CCGGC 108 GCCGGC PpuMI 1021 GGGTCCC [GA]G^G[AT]CC[CT] 1057 GGGTCCC RsaI 435 GTAC GT^AC 864 GTAC SapI 477 GCTCTTC GCTCTTC(1/4) 1044 GCTCTTC SfoI 36 GGCGCC GGC^GCC 105 GGCGCC SmaI 581 CCCGGG CCC^GGG 1061 CCCGGG Tsp509I 772 AATT ^AATT 781 AATT XcmI 707 CCAAACTGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 848 CCATCGTGACGCTGG XmaCI 581 CCCGGG C^CCGGG 1061 CCCGGG XmaI 581 CCCGGG C^CCGGG 1061 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI AvaII BalI BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I