(Linear) (Six-base) MAPSORT of: KIEE1478.seq Check: 4985 from: 1 to: 1521 KIEE1478.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 496 1082 1491 1521 Size: 496 586 409 30 Fragments arranged by size: 586 496 409 30 AccI GT'mk_AC Cuts at: 0 368 1412 1521 Size: 368 1044 109 Fragments arranged by size: 1044 368 109 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1182 1521 Size: 1182 339 AflIII A'CryG_T Cuts at: 0 1138 1521 Size: 1138 383 AleI CACnn'nnGTG Cuts at: 0 220 550 1117 1141 1521 Size: 220 330 567 24 380 Fragments arranged by size: 567 380 330 220 24 AlwNI CAG_nnn'CTG Cuts at: 0 915 1521 Size: 915 606 ApaI G_GGCC'C Cuts at: 0 16 1521 Size: 16 1505 ApoI r'AATT_y Cuts at: 0 780 1521 Size: 780 741 AvaI C'yCGr_G Cuts at: 0 195 499 581 1061 1521 Size: 195 304 82 480 460 Fragments arranged by size: 480 460 304 195 82 BanI G'GyrC_C Cuts at: 0 36 105 319 495 703 1521 Size: 36 69 214 176 208 818 Fragments arranged by size: 818 214 208 176 69 36 BanII G_rGCy'C Cuts at: 0 16 317 1521 Size: 16 301 1204 Fragments arranged by size: 1204 301 16 BbeI G_GCGC'C Cuts at: 0 40 109 1521 Size: 40 69 1412 Fragments arranged by size: 1412 69 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 273 444 978 1363 1521 Size: 273 171 534 385 158 Fragments arranged by size: 534 385 273 171 158 BbvCI CC'TCA_GC Cuts at: 0 1396 1521 Size: 1396 125 BclI T'GATC_A Cuts at: 0 629 800 1521 Size: 629 171 721 Fragments arranged by size: 721 629 171 BglI GCCn_nnn'nGGC Cuts at: 0 1449 1521 Size: 1449 72 Bme1580I G_kGCm'C Cuts at: 0 16 500 1521 Size: 16 484 1021 Fragments arranged by size: 1021 484 16 BmtI G_CTAG'C Cuts at: 0 34 1521 Size: 34 1487 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 255 1521 Size: 255 1266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1023 1441 1521 Size: 382 641 418 80 Fragments arranged by size: 641 418 382 80 Bpu10I CC'TnA_GC Cuts at: 0 1268 1396 1521 Size: 1268 128 125 Fragments arranged by size: 1268 128 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 376 454 1294 1521 Size: 211 165 78 840 227 Fragments arranged by size: 840 227 211 165 78 BsaAI yAC'GTr Cuts at: 0 867 1139 1521 Size: 867 272 382 Fragments arranged by size: 867 382 272 BsaHI Gr'CG_yC Cuts at: 0 37 106 211 278 962 1521 Size: 37 69 105 67 684 559 Fragments arranged by size: 684 559 105 69 67 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1063 1093 1150 1165 1180 1195 1521 Size: 1063 30 57 15 15 15 326 Fragments arranged by size: 1063 326 57 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 64 517 1099 1444 1521 Size: 64 453 582 345 77 Fragments arranged by size: 582 453 345 77 64 BseYI C'CCAG_C Cuts at: 0 639 1521 Size: 639 882 BsiEI CG_ry'CG Cuts at: 0 47 551 1478 1521 Size: 47 504 927 43 Fragments arranged by size: 927 504 47 43 BsiHKAI G_wGCw'C Cuts at: 0 227 317 1521 Size: 227 90 1204 Fragments arranged by size: 1204 227 90 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1350 1521 Size: 1350 171 Bsp1286I G_dGCh'C Cuts at: 0 16 227 317 500 1521 Size: 16 211 90 183 1021 Fragments arranged by size: 1021 211 183 90 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 473 496 827 1082 1491 1521 Size: 473 23 331 255 409 30 Fragments arranged by size: 473 409 331 255 30 23 BsrBI CCG'CTC Cuts at: 0 477 1278 1521 Size: 477 801 243 Fragments arranged by size: 801 477 243 BsrFI r'CCGG_y Cuts at: 0 39 108 1521 Size: 39 69 1413 Fragments arranged by size: 1413 69 39 BssHII G'CGCG_C Cuts at: 0 24 26 296 1521 Size: 24 2 270 1225 Fragments arranged by size: 1225 270 24 2 BssSI C'ACGA_G Cuts at: 0 517 1129 1521 Size: 517 612 392 Fragments arranged by size: 612 517 392 BstXI CCAn_nnnn'nTGG Cuts at: 0 177 519 615 1521 Size: 177 342 96 906 Fragments arranged by size: 906 342 177 96 BstYI r'GATC_y Cuts at: 0 681 1521 Size: 681 840 BtgI C'CryG_G Cuts at: 0 215 929 935 1364 1521 Size: 215 714 6 429 157 Fragments arranged by size: 714 429 215 157 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 413 1521 Size: 413 1108 EaeI y'GGCC_r Cuts at: 0 44 80 167 548 827 932 1229 1521 Size: 44 36 87 381 279 105 297 292 Fragments arranged by size: 381 297 292 279 105 87 44 36 EagI C'GGCC_G Cuts at: 0 44 548 1521 Size: 44 504 973 Fragments arranged by size: 973 504 44 EarI CTCTTCn'nnn_ Cuts at: 0 53 484 1051 1336 1521 Size: 53 431 567 285 185 Fragments arranged by size: 567 431 285 185 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 356 1521 Size: 356 1165 EcoICRI GAG'CTC Cuts at: 0 315 1521 Size: 315 1206 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1023 1182 1441 1521 Size: 382 641 159 259 80 Fragments arranged by size: 641 382 259 159 80 EcoO109I rG'GnC_Cy Cuts at: 0 1022 1058 1521 Size: 1022 36 463 Fragments arranged by size: 1022 463 36 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 495 1194 1521 Size: 495 699 327 Fragments arranged by size: 699 495 327 HaeII r_GCGC'y Cuts at: 0 40 109 256 1521 Size: 40 69 147 1265 Fragments arranged by size: 1265 147 69 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1165 1197 1521 Size: 1165 32 324 Fragments arranged by size: 1165 324 32 KasI G'GCGC_C Cuts at: 0 36 105 1521 Size: 36 69 1416 Fragments arranged by size: 1416 69 36 MscI TGG'CCA Cuts at: 0 169 829 1231 1521 Size: 169 660 402 290 Fragments arranged by size: 660 402 290 169 MslI CAynn'nnrTG Cuts at: 0 175 205 220 550 1117 1141 1240 1288 Size: 175 30 15 330 567 24 99 48 Cuts at: 1288 1501 1521 Size: 213 20 Fragments arranged by size: 567 330 213 175 99 48 30 24 20 15 MspA1I CmG'CkG Cuts at: 0 141 746 1400 1521 Size: 141 605 654 121 Fragments arranged by size: 654 605 141 121 NaeI GCC'GGC Cuts at: 0 41 110 1521 Size: 41 69 1411 Fragments arranged by size: 1411 69 41 NarI GG'CG_CC Cuts at: 0 37 106 1521 Size: 37 69 1415 Fragments arranged by size: 1415 69 37 NgoMIV G'CCGG_C Cuts at: 0 39 108 1521 Size: 39 69 1413 Fragments arranged by size: 1413 69 39 NheI G'CTAG_C Cuts at: 0 30 1521 Size: 30 1491 NspI r_CATG'y Cuts at: 0 360 1521 Size: 360 1161 PfoI T'CCnGG_A Cuts at: 0 587 970 1521 Size: 587 383 551 Fragments arranged by size: 587 551 383 PmlI CAC'GTG Cuts at: 0 1139 1521 Size: 1139 382 PpuMI rG'GwC_Cy Cuts at: 0 1022 1058 1521 Size: 1022 36 463 Fragments arranged by size: 1022 463 36 PspOMI G'GGCC_C Cuts at: 0 12 1521 Size: 12 1509 PstI C_TGCA'G Cuts at: 0 140 1521 Size: 140 1381 SacI G_AGCT'C Cuts at: 0 317 1521 Size: 317 1204 SanDI GG'GwC_CC Cuts at: 0 1022 1058 1521 Size: 1022 36 463 Fragments arranged by size: 1022 463 36 SapI GCTCTTCn'nnn_ Cuts at: 0 484 1051 1521 Size: 484 567 470 Fragments arranged by size: 567 484 470 SfcI C'TryA_G Cuts at: 0 136 561 810 1521 Size: 136 425 249 711 Fragments arranged by size: 711 425 249 136 SfoI GGC'GCC Cuts at: 0 38 107 1521 Size: 38 69 1414 Fragments arranged by size: 1414 69 38 SgrAI Cr'CCGG_yG Cuts at: 0 39 1521 Size: 39 1482 SmaI CCC'GGG Cuts at: 0 583 1063 1521 Size: 583 480 458 Fragments arranged by size: 583 480 458 SmlI C'TyrA_G Cuts at: 0 226 391 469 1273 1521 Size: 226 165 78 804 248 Fragments arranged by size: 804 248 226 165 78 StyI C'CwwG_G Cuts at: 0 1169 1521 Size: 1169 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1115 1521 Size: 1115 406 TatI w'GTAC_w Cuts at: 0 434 1521 Size: 434 1087 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1063 1095 1521 Size: 1063 32 426 Fragments arranged by size: 1063 426 32 XbaI T'CTAG_A Cuts at: 0 678 1521 Size: 678 843 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 714 855 1521 Size: 714 141 666 Fragments arranged by size: 714 666 141 XmaI C'CCGG_G Cuts at: 0 581 1061 1521 Size: 581 480 460 Fragments arranged by size: 581 480 460 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PfoI PmlI PpuMI PspOMI PstI SacI SanDI SapI SfcI SfoI SgrAI SmaI SmlI StyI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BsaI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmnI ZraI