################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NciI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 930 CGCG CG^CG AcyI 1435 GGCGTC G[GA]^CG[CT]C AfaI 420 GTAC GT^AC AhdI 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1185 GGGCCC GGGCC^C AspEI 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 200 TGGCCA TGG^CCA BciVI 703 GTATCC GTATCC(6/5) BlpI 664 GCTAAGC GC^T[ACGT]AGC Bpu1102I 664 GCTAAGC GC^T[ACGT]AGC BsaHI 1435 GGCGTC G[GA]^CG[CT]C BseRI 655 GAGGAG GAGGAG(10/8) BsmBI 179 CGTCTC CGTCTC(1/5) BsmI 660 GAATGC GAATGC(1/-1) Bsp1407I 419 TGTACA T^GTACA BspHI 1043 TCATGA T^CATGA BspMI 895 GCAGGT ACCTGC(4/8) BsrGI 419 TGTACA T^GTACA BstAPI 1405 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 15 GGTCACC G^GT[ACGT]ACC BstPI 15 GGTCACC G^GT[ACGT]ACC BstUI 930 CGCG CG^CG BstXI 507 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 664 GCTAAGC GC^T[ACGT]AGC DraIII 1406 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1359 CCGTGG C^C[GA][CT]GG Eam1105I 245 GACTCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 15 GGTCACC G^GT[ACGT]ACC EcoRV 1378 GATATC GAT^ATC HgaI 1436 GCGTC GACGC(5/10) Hin1I 1435 GGCGTC G[GA]^CG[CT]C MluNI 200 TGGCCA TGG^CCA MscI 200 TGGCCA TGG^CCA MvnI 930 CGCG CG^CG NaeI 1213 GCCGGC GCC^GGC NdeI 1003 CATATG CA^TATG NgoMIV 1213 GCCGGC G^CCGGC PflFI 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1148 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1043 TCATGA T^CATGA RsaI 420 GTAC GT^AC SacI 775 GAGCTC GAGCT^C SfcI 956 CTGTAG C^T[GA][CT]AG SspBI 419 TGTACA T^GTACA StuI 1011 AGGCCT AGG^CCT TaqI 1457 TCGA T^CGA TfiI 881 GAATC G^A[AT]TC Tth111I 1432 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1457 TCGA T^CGA Van91I 1148 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 775 GAGCTC G[AT]GC[AT]^C 823 GTGCTC BanI 454 GGTGCC G^G[CT][GA]CC 691 GGCACC BfaI 772 CTAG C^TAG 1243 CTAG BpmI 676 CTCCAG CTGGAG(16/14) 826 CTCCAG BsaI 1354 GAGACC GGTCTC(1/5) 1471 GAGACC BsiHKAI 775 GAGCTC G[AT]GC[AT]^C 823 GTGCTC BsrFI 1213 GCCGGC [GA]^CCGG[CT] 1306 ACCGGC CfoI 270 GCGC GCG^C 929 GCGC Cfr10I 1213 GCCGGC [GA]^CCGG[CT] 1306 ACCGGC EcoRI 494 GAATTC G^AATTC 523 GAATTC EcoT14I 531 CCTTGG C^C[AT][AT]GG 1014 CCTTGG HapII 1214 CCGG C^CGG 1307 CCGG HhaI 270 GCGC GCG^C 929 GCGC HinP1I 270 GCGC G^CGC 929 GCGC HincII 253 GTCAAC GT[CT]^[GA]AC 289 GTCAAC HindII 253 GTCAAC GT[CT]^[GA]AC 289 GTCAAC HpaII 1214 CCGG C^CGG 1307 CCGG MaeI 772 CTAG C^TAG 1243 CTAG MaeII 178 ACGT A^CGT 1289 ACGT MslI 1038 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1440 CATGGAGATG MspI 1214 CCGG C^CGG 1307 CCGG PvuII 475 CAGCTG CAG^CTG 1196 CAGCTG SapI 541 GAAGAGC GCTCTTC(1/4) 1254 GCTCTTC StyI 531 CCTTGG C^C[AT][AT]GG 1014 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BanII BbvI BglII BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI XhoII