(Circular) (Six-base) MAPSORT of: pFN21AE4478.seq Check: 9504 from: 1 to: 6193 pFN21AE4478 6193 bp With 182 enzymes: SgfI * March 28, 2012 14:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2891 2891 Size: 6193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2565 3166 4256 5640 5908 1632 Size: 933 601 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1090 933 601 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 2255 4922 2255 Size: 2667 3526 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 5077 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaI G_GGCC'C Cuts at: 3194 3194 Size: 6193 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2123 2499 2528 3502 3728 3880 5989 1493 Size: 630 376 29 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 630 376 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2459 2696 3514 4969 618 Size: 325 217 627 672 237 818 1455 1842 Fragments arranged by size: 1842 1455 818 672 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2784 2916 3194 3512 1391 Size: 1393 132 278 318 4072 Fragments arranged by size: 4072 1393 318 278 132 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2719 4085 5295 2719 Size: 1366 1210 3617 Fragments arranged by size: 3617 1366 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2436 2514 3425 6188 2436 Size: 78 911 2763 2441 Fragments arranged by size: 2763 2441 911 78 BlpI GC'TnA_GC Cuts at: 2670 3610 2670 Size: 940 5253 Bme1580I G_kGCm'C Cuts at: 1790 2421 2699 3194 4254 5410 1790 Size: 631 278 495 1060 1156 2573 Fragments arranged by size: 2573 1156 1060 631 495 278 BmgBI CAC'GTC Cuts at: 1318 2184 1318 Size: 866 5327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2097 2576 3924 Size: 416 53 83 399 24 680 479 1348 Cuts at: 3924 4882 442 Size: 958 1753 Fragments arranged by size: 1753 1348 958 680 479 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2666 2816 4853 1239 Size: 1427 150 2037 2579 Fragments arranged by size: 2579 2037 1427 150 Bpu10I CC'TnA_GC Cuts at: 3471 3471 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3353 3470 4856 882 Size: 617 1854 117 1386 2219 Fragments arranged by size: 2219 1854 1386 617 117 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3441 4000 4382 5017 Size: 53 83 186 936 1908 559 382 635 Cuts at: 5017 275 Size: 1451 Fragments arranged by size: 1908 1451 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3221 3251 3279 3309 1178 Size: 30 165 30 1818 30 28 30 4062 Fragments arranged by size: 4062 1818 165 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2675 1186 Size: 1489 4704 BseYI C'CCAG_C Cuts at: 2026 2482 3199 5396 5892 2026 Size: 456 717 2197 496 2327 Fragments arranged by size: 2327 2197 717 496 456 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 2622 3438 1163 Size: 573 653 233 816 3918 Fragments arranged by size: 3918 816 653 573 233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2784 2832 4254 4339 5410 2784 Size: 48 1422 85 1071 3567 Fragments arranged by size: 3567 1422 1071 85 48 BsmI GAATG_Cn' Cuts at: 2671 3704 3797 2671 Size: 1033 93 5067 Fragments arranged by size: 5067 1033 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2190 2190 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2421 2699 2784 2832 2916 3194 3512 Size: 399 631 278 85 48 84 278 318 Cuts at: 3512 4254 4339 5410 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 631 399 318 278 278 85 85 84 48 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 3048 4082 5812 3048 Size: 1034 1730 3429 Fragments arranged by size: 3429 1730 1034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 3548 844 Size: 2047 657 3489 Fragments arranged by size: 3489 2047 657 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3218 3311 4837 5024 1700 Size: 110 1408 93 1526 187 2869 Fragments arranged by size: 2869 1526 1408 187 110 93 BsrGI T'GTAC_A Cuts at: 96 2424 96 Size: 2328 3865 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 3416 794 Size: 2622 3571 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 2020 1184 Size: 836 5357 BstXI CCAn_nnnn'nTGG Cuts at: 2519 2519 Size: 6193 BstYI r'GATC_y Cuts at: 1972 2436 2514 2676 3425 3523 3890 4275 4292 Size: 464 78 162 749 98 367 385 17 Cuts at: 4292 5744 5830 5842 6188 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 749 464 385 367 346 162 98 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3364 513 Size: 552 939 1360 3342 Fragments arranged by size: 3342 1360 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3221 487 Size: 683 56 1995 3459 Fragments arranged by size: 3459 1995 683 56 BtsI GCAGTG_nn' Cuts at: 3219 3709 4503 4523 3219 Size: 490 794 20 4889 Fragments arranged by size: 4889 794 490 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DraIII CAC_nnn'GTG Cuts at: 3416 3416 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2555 3171 Size: 54 1425 50 275 18 375 350 616 Cuts at: 3171 3216 3640 4529 6158 8 Size: 45 424 889 1629 43 Fragments arranged by size: 1629 1425 889 616 424 375 350 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 2396 2541 2652 3185 3266 4123 1353 Size: 905 138 145 111 533 81 857 3423 Fragments arranged by size: 3423 905 857 533 145 138 111 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2782 2782 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 2666 2816 3166 4256 4853 5640 Size: 393 933 101 150 350 1090 597 787 Cuts at: 5640 5908 1239 Size: 268 1524 Fragments arranged by size: 1524 1090 933 787 597 393 350 268 150 101 EcoO109I rG'GnC_Cy Cuts at: 3149 3190 3191 3149 Size: 41 1 6151 Fragments arranged by size: 6151 41 1 EcoRI G'AATT_C Cuts at: 2499 2528 3502 3880 2499 Size: 29 974 378 4812 Fragments arranged by size: 4812 974 378 29 EcoRV GAT'ATC Cuts at: 3385 3385 Size: 6193 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2356 2388 2506 Size: 32 135 32 669 32 83 32 118 Cuts at: 2506 2538 2625 2657 2796 2828 4834 4866 4908 Size: 32 87 32 139 32 2006 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2626 2006 669 139 135 118 87 83 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 2296 3537 3783 669 Size: 988 281 322 36 1241 246 3079 Fragments arranged by size: 3079 1241 988 322 281 246 36 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2227 2476 3232 3252 3264 5307 5491 1305 Size: 922 249 756 20 12 2043 184 2007 Fragments arranged by size: 2043 2007 922 756 249 184 20 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2207 10 Size: 54 1425 343 375 3996 Fragments arranged by size: 3996 1425 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3047 3449 4151 4510 4669 518 Size: 648 1881 402 702 359 159 2042 Fragments arranged by size: 2042 1881 702 648 402 359 159 MspA1I CmG'CkG Cuts at: 1606 2482 2560 2686 3203 3272 3609 4286 5434 Size: 876 78 126 517 69 337 677 1148 Cuts at: 5434 5679 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 876 677 517 337 245 126 78 69 NaeI GCC'GGC Cuts at: 3220 3220 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 NdeI CA'TA_TG Cuts at: 387 3009 387 Size: 2622 3571 NgoMIV G'CCGG_C Cuts at: 3218 3218 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3551 5096 1132 Size: 97 2322 1545 2229 Fragments arranged by size: 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 3159 1108 Size: 2051 4142 PfoI T'CCnGG_A Cuts at: 2721 2721 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 3190 3190 Size: 6193 PstI C_TGCA'G Cuts at: 830 3545 830 Size: 2715 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2482 3203 2482 Size: 721 5472 SacI G_AGCT'C Cuts at: 2784 2784 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SapI GCTCTTCn'nnn_ Cuts at: 2541 3266 2541 Size: 725 5468 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2961 3541 4676 5357 5548 826 Size: 219 1916 580 1135 681 191 1471 Fragments arranged by size: 1916 1471 1135 681 580 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4297 5198 5460 820 Size: 197 926 2354 901 262 1553 Fragments arranged by size: 2354 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 3018 1517 Size: 1501 4692 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 3019 3632 513 Size: 552 939 532 483 613 3074 Fragments arranged by size: 3074 939 613 552 532 483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 4220 4237 4390 4575 5020 325 Size: 1255 1134 1506 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 1134 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 4439 6151 Size: 274 80 33 51 1443 447 2015 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 2015 1712 1443 447 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 6153 6185 2490 Size: 32 3631 32 2498 Fragments arranged by size: 3631 2498 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3440 1316 Size: 2124 4069 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI