################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BsaAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HinfI HpaI KasI KspI MaeI MfeI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 655 GACGTC GACGT^C Acc65I 455 GGTACC G^GTACC AccIII 1007 TCCGGA T^CCGGA AcyI 655 GACGTC G[GA]^CG[CT]C Asp718I 455 GGTACC G^GTACC AviII 555 TGCGCA TGC^GCA BanI 455 GGTACC G^G[CT][GA]CC BglI 583 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1202 AGATCT A^GATCT BlpI 1050 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1050 GCTCAGC GC^T[ACGT]AGC BsaBI 714 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 655 GACGTC G[GA]^CG[CT]C BsaWI 1007 TCCGGA [AT]^CCGG[AT] BseAI 1007 TCCGGA T^CCGGA BsgI 827 CTGCAC GTGCAG(16/14) BsmBI 905 GAGACG CGTCTC(1/5) BsmI 552 GAATGC GAATGC(1/-1) BspEI 1007 TCCGGA T^CCGGA BspMI 7 ACCTGC ACCTGC(4/8) BsrDI 534 CATTGC GCAATG(2/0) BsrFI 796 ACCGGC [GA]^CCGG[CT] BssSI 763 CACGAG CACGAG(-5/-1) BstEII 354 GGTGACC G^GT[ACGT]ACC BstPI 354 GGTGACC G^GT[ACGT]ACC CelII 1050 GCTCAGC GC^T[ACGT]AGC Cfr10I 796 ACCGGC [GA]^CCGG[CT] EarI 1347 CTCTTC CTCTTC(1/4) EcoO65I 354 GGTGACC G^GT[ACGT]ACC EcoT14I 267 CCTTGG C^C[AT][AT]GG FspI 555 TGCGCA TGC^GCA Hin1I 655 GACGTC G[GA]^CG[CT]C KpnI 455 GGTACC GGTAC^C Ksp632I 1347 CTCTTC CTCTTC(1/4) MamI 714 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1007 TCCGGA T^CCGGA NspI 1106 GCATGC [GA]CATG^[CT] PflMI 1020 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 487 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 916 ACCTGGT A^CC[AT]GGT SmlI 1375 CTCAAG C^T[CT][GA]AG SphI 1106 GCATGC GCATG^C StuI 726 AGGCCT AGG^CCT StyI 267 CCTTGG C^C[AT][AT]GG Van91I 1020 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 1223 CGCG CG^CG 1321 CGCG AlwI 444 GATCC GGATC(4/5) 1122 GATCC AlwNI 1021 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1126 CAGGGACTG AocI 519 CCTGAGG CC^T[ACGT]AGG 750 CCTGAGG BalI 695 TGGCCA TGG^CCA 1302 TGGCCA BbsI 1218 GAAGAC GAAGAC(2/6) 1327 GTCTTC BcnI 672 CCGGG CC^[GC]GG 1364 CCCGG BpuAI 1218 GAAGAC GAAGAC(2/6) 1327 GTCTTC BsaI 913 GAGACC GGTCTC(1/5) 1087 GAGACC BstUI 1223 CGCG CG^CG 1321 CGCG Bsu36I 519 CCTGAGG CC^T[ACGT]AGG 750 CCTGAGG CfoI 556 GCGC GCG^C 669 GCGC Eco81I 519 CCTGAGG CC^T[ACGT]AGG 750 CCTGAGG FauI 1319 CCCGC CCCGC(4/6) 185 GCGGG HgaI 907 GACGC GACGC(5/10) 1221 GACGC HhaI 556 GCGC GCG^C 669 GCGC HinP1I 556 GCGC G^CGC 669 GCGC MaeII 656 ACGT A^CGT 812 ACGT MluNI 695 TGGCCA TGG^CCA 1302 TGGCCA MscI 695 TGGCCA TGG^CCA 1302 TGGCCA MvnI 1223 CGCG CG^CG 1321 CGCG NciI 672 CCGGG CC^[GC]GG 1364 CCCGG PpuMI 112 GGGTCCT [GA]G^G[AT]CC[CT] 515 AGGACCT PstI 9 CTGCAG CTGCA^G 1039 CTGCAG PvuII 1111 CAGCTG CAG^CTG 1153 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII