(Circular) (Six-base) MAPSORT of: pFN21AB6678.seq Check: 9082 from: 1 to: 6013 pFN21AB6678 6013 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 2071 2342 3143 4076 5460 5728 1632 Size: 427 12 271 801 933 1384 268 1917 Fragments arranged by size: 1917 1384 933 801 427 271 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 3006 4912 5889 1203 Size: 1803 1906 977 1327 Fragments arranged by size: 1906 1803 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2955 2987 2955 Size: 32 5981 AlwNI CAG_nnn'CTG Cuts at: 2191 2426 3756 5328 2191 Size: 235 1330 1572 2876 Fragments arranged by size: 2876 1572 1330 235 ApaI G_GGCC'C Cuts at: 2054 2054 Size: 6013 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 3322 3548 3700 5809 1493 Size: 1829 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2053 2098 3338 1870 Size: 73 104 6 45 1240 4545 Fragments arranged by size: 4545 1240 104 73 45 6 BamHI G'GATC_C Cuts at: 2512 3343 3710 2512 Size: 831 367 4815 Fragments arranged by size: 4815 831 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 4789 618 Size: 325 217 627 1547 1455 1842 Fragments arranged by size: 1842 1547 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2105 3332 1391 Size: 663 51 1227 4072 Fragments arranged by size: 4072 1227 663 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2348 928 Size: 971 449 4593 Fragments arranged by size: 4593 971 449 BbvCI CC'TCA_GC Cuts at: 2391 2391 Size: 6013 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 4624 136 Size: 107 122 71 631 605 367 2585 1525 Fragments arranged by size: 2585 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2054 4074 5230 1790 Size: 264 2020 1156 2573 Fragments arranged by size: 2573 2020 1156 264 BmgBI CAC'GTC Cuts at: 1318 3092 1318 Size: 1774 4239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3200 3744 4702 Size: 416 53 83 399 24 1783 544 958 Cuts at: 4702 442 Size: 1753 Fragments arranged by size: 1783 1753 958 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 4673 1239 Size: 850 2584 2579 Fragments arranged by size: 2584 2579 850 Bpu10I CC'TnA_GC Cuts at: 2391 2391 Size: 6013 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3032 3111 4676 882 Size: 617 1533 79 1565 2219 Fragments arranged by size: 2219 1565 1533 617 79 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 2435 6007 2435 Size: 3572 2441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2114 2716 3820 4202 Size: 53 83 186 936 581 602 1104 382 Cuts at: 4202 4837 275 Size: 635 1451 Fragments arranged by size: 1451 1104 936 635 602 581 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2955 2985 1178 Size: 30 165 30 684 30 838 30 4206 Fragments arranged by size: 4206 838 684 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2976 1186 Size: 1790 4223 BseYI C'CCAG_C Cuts at: 2923 3272 5216 5712 2923 Size: 349 1944 496 3224 Fragments arranged by size: 3224 1944 496 349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3003 1163 Size: 573 1267 4173 Fragments arranged by size: 4173 1267 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4074 4159 5230 4074 Size: 85 1071 4857 Fragments arranged by size: 4857 1071 85 BsmI GAATG_Cn' Cuts at: 2215 2857 3524 3617 2215 Size: 642 667 93 4611 Fragments arranged by size: 4611 667 642 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2105 3332 4074 4159 5230 1391 Size: 399 264 51 1227 742 85 1071 2174 Fragments arranged by size: 2174 1227 1071 742 399 264 85 51 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3368 844 Size: 2524 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2173 3900 1394 Size: 429 350 1727 3507 Fragments arranged by size: 3507 1727 429 350 BsrDI GCAATG_nn' Cuts at: 65 1248 2680 2862 4508 4682 65 Size: 1183 1432 182 1646 174 1396 Fragments arranged by size: 1646 1432 1396 1183 182 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2069 4657 4844 1700 Size: 110 232 27 2588 187 2869 Fragments arranged by size: 2869 2588 232 187 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 2062 2153 1925 Size: 137 91 5785 Fragments arranged by size: 5785 137 91 BssSI C'ACGA_G Cuts at: 3030 3248 4073 5085 3030 Size: 218 825 1012 3958 Fragments arranged by size: 3958 1012 825 218 BstAPI GCAn_nnn'nTGC Cuts at: 794 2251 794 Size: 1457 4556 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2512 3343 3710 4095 4112 5564 5650 5662 Size: 540 831 367 385 17 1452 86 12 Cuts at: 5662 6008 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 831 540 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5461 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3057 3313 3529 4323 4343 3057 Size: 256 216 794 20 4727 Fragments arranged by size: 4727 794 256 216 20 ClaI AT'CG_AT Cuts at: 1554 2551 1554 Size: 997 5016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2781 2816 529 Size: 35 2217 35 3726 Fragments arranged by size: 3726 2217 35 35 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DraIII CAC_nnn'GTG Cuts at: 2833 2833 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3266 5020 809 Size: 507 1950 1754 1802 Fragments arranged by size: 1950 1802 1754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 3460 4349 Size: 54 1425 50 275 18 210 1420 889 Cuts at: 4349 5978 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3943 1353 Size: 2590 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2071 2089 2342 3143 4076 4673 Size: 393 427 12 18 253 801 933 597 Cuts at: 4673 5460 5728 1239 Size: 787 268 1524 Fragments arranged by size: 1524 933 801 787 597 427 393 268 253 18 12 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2518 2518 Size: 6013 FseI GG_CCGG'CC Cuts at: 2046 2046 Size: 6013 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2186 2218 4654 Size: 32 135 32 515 32 67 32 2436 Cuts at: 4654 4686 4728 4760 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2436 515 135 67 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3357 3603 669 Size: 988 281 1245 174 246 3079 Fragments arranged by size: 3079 1245 988 281 246 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 2327 3612 2327 Size: 1285 4728 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5127 5311 1305 Size: 3822 184 2007 Fragments arranged by size: 3822 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2454 3971 4330 4489 518 Size: 648 1288 1517 359 159 2042 Fragments arranged by size: 2042 1517 1288 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3429 4106 5254 5499 1606 Size: 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 245 NaeI GCC'GGC Cuts at: 2044 2071 2044 Size: 27 5986 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5461 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2042 2069 2042 Size: 27 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 3010 3371 4916 1132 Size: 97 1781 361 1545 2229 Fragments arranged by size: 2229 1781 1545 361 97 PasI CC'CwG_GG Cuts at: 1348 2476 1348 Size: 1128 4885 PciI A'CATG_T Cuts at: 3006 4912 3006 Size: 1906 4107 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2955 2987 4082 4114 5595 5627 2955 Size: 32 1095 32 1481 32 3341 Fragments arranged by size: 3341 1481 1095 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 2050 2050 Size: 6013 PstI C_TGCA'G Cuts at: 830 3365 830 Size: 2535 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 2540 4261 5973 2540 Size: 1721 1712 2580 Fragments arranged by size: 2580 1721 1712 SfcI C'TryA_G Cuts at: 826 1045 3361 4496 5177 5368 826 Size: 219 2316 1135 681 191 1471 Fragments arranged by size: 2316 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 2049 2055 3340 1872 Size: 177 6 1285 4545 Fragments arranged by size: 4545 1285 177 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5018 5280 820 Size: 197 926 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SrfI GCCC'GGGC Cuts at: 2049 2049 Size: 6013 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2037 3112 3452 513 Size: 552 972 1075 340 3074 Fragments arranged by size: 3074 1075 972 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2973 4040 4057 4210 4395 4840 325 Size: 1255 1393 1067 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1255 1067 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 2677 4259 Size: 274 80 33 51 1443 561 139 1582 Cuts at: 4259 5971 96 Size: 1712 138 Fragments arranged by size: 1712 1582 1443 561 274 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3093 3125 5973 6005 3093 Size: 32 2848 32 3101 Fragments arranged by size: 3101 2848 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 2047 2053 3338 1870 Size: 177 6 1285 4545 Fragments arranged by size: 4545 1285 177 6 XmnI GAAnn'nnTTC Cuts at: 2186 4142 2186 Size: 1956 4057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI