################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MamI MluNI MscI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AclI 1073 AACGTT AA^CGTT AlwI 336 GGATC GGATC(4/5) AlwNI 518 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 1031 CCCGAG C^[CT]CG[GA]G AvaII 23 GGTCC G^G[AT]CC BmyI 812 GTGCCC G[AGT]GC[ACT]^C BseRI 364 GAGGAG GAGGAG(10/8) BsmAI 1052 GTCTC GTCTC(1/5) BsoBI 1031 CCCGAG C^[CT]CG[GA]G Bsp1286I 812 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 1149 TGTACA T^GTACA BspLU11I 1175 ACATGT A^CATGT BsrDI 511 GCAATG GCAATG(2/0) BsrGI 1149 TGTACA T^GTACA BsrI 581 ACTGG ACTGG(1/-1) BstBI 146 TTCGAA TT^CGAA BstYI 336 GGATCT [GA]^GATC[CT] DpnII 337 GATC ^GATC DraII 22 GGGTCCC [GA]G^G[ACGT]CC[CT] EarI 826 GAAGAG CTCTTC(1/4) EcoO109I 22 GGGTCCC [GA]G^G[ACGT]CC[CT] FauI 67 CCCGC CCCGC(4/6) HgaI 32 GCGTC GACGC(5/10) HphI 975 TCACC GGTGA(8/7) Ksp632I 826 GAAGAG CTCTTC(1/4) MboI 337 GATC ^GATC MfeI 1190 CAATTG C^AATTG MflI 336 GGATCT [GA]^GATC[CT] MluI 30 ACGCGT A^CGCGT MunI 1190 CAATTG C^AATTG NdeII 337 GATC ^GATC NspI 1175 ACATGT [GA]CATG^[CT] NspV 146 TTCGAA TT^CGAA PflMI 1225 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1081 GACTC GAGTC(4/5) PpuMI 22 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 1045 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1073 AACGTT AA^CGTT PstI 485 CTGCAG CTGCA^G Sau3AI 337 GATC ^GATC SfuI 146 TTCGAA TT^CGAA SspBI 1149 TGTACA T^GTACA TaqI 147 TCGA T^CGA TthHB8I 147 TCGA T^CGA Van91I 1225 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 105 TCTAGA T^CTAGA XcmI 1088 CCACTGCTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 336 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 88 TCCGGA T^CCGGA 179 TCCGGA AcsI 143 GAATTC [GA]^AATT[CT] 910 GAATTC AflIII 30 ACGCGT A^C[GA][CT]GT 1175 ACATGT ApoI 143 GAATTC [GA]^AATT[CT] 910 GAATTC BciVI 768 GGATAC GTATCC(6/5) 1171 GGATAC BsaWI 88 TCCGGA [AT]^CCGG[AT] 179 TCCGGA BseAI 88 TCCGGA T^CCGGA 179 TCCGGA BsmFI 732 GGGAC GGGAC(10/14) 24 GTCCC BspEI 88 TCCGGA T^CCGGA 179 TCCGGA BspHI 660 TCATGA T^CATGA 851 TCATGA BstF5I 570 GGATG GGATG(2/0) 1209 GGATG Cfr13I 23 GGTCC G^G[ACGT]CC 391 GGCCC EaeI 2 TGGCCG [CT]^GGCC[GA] 42 CGGCCA EcoRI 143 GAATTC G^AATTC 910 GAATTC FokI 570 GGATG GGATG(9/13) 1209 GGATG HapII 89 CCGG C^CGG 180 CCGG HpaII 89 CCGG C^CGG 180 CCGG MaeII 102 ACGT A^CGT 1074 ACGT MroI 88 TCCGGA T^CCGGA 179 TCCGGA MspA1I 61 CAGCTG C[AC]G^C[GT]G 1057 CAGCTG MspI 89 CCGG C^CGG 180 CCGG NlaIV 22 GGGTCC GG[ACGT]^[ACGT]CC 23 GGTCCC PvuII 61 CAGCTG CAG^CTG 1057 CAGCTG RcaI 660 TCATGA T^CATGA 851 TCATGA Sau96I 23 GGTCC G^G[ACGT]CC 391 GGCCC SfcI 485 CTGCAG C^T[GA][CT]AG 979 CTACAG SspI 304 AATATT AAT^ATT 1106 AATATT TspRI 986 CAGTG CA[GC]TG[ACGT][ACGT]^ 1089 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AfaI AluI Asp700I BbvI BfaI BpmI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI DdeI DsaI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HindIII HinfI ItaI MaeI MaeIII MboII MnlI MseI MslI MvaI MvnI MwoI NcoI NlaIII RsaI ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I XmnI