(Circular) (Six-base) MAPSORT of: pFN21AE6326.seq Check: 8063 from: 1 to: 5995 pFN21AE6326 5995 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 3079 4122 4495 1563 Size: 1516 1043 373 3063 Fragments arranged by size: 3063 1516 1043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 5442 5710 1632 Size: 2426 1384 268 1917 Fragments arranged by size: 2426 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 2035 3180 4894 5871 1203 Size: 832 1145 1714 977 1327 Fragments arranged by size: 1714 1327 1145 977 832 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2528 3738 5310 2528 Size: 1210 1572 3213 Fragments arranged by size: 3213 1572 1210 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2148 2915 3304 3530 3682 5791 1493 Size: 655 767 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 655 389 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3036 3320 1870 Size: 73 1093 284 4545 Fragments arranged by size: 4545 1093 284 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3314 1391 Size: 1923 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2767 3170 3887 5097 2767 Size: 403 717 1210 3665 Fragments arranged by size: 3665 1210 717 403 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 2821 4056 5212 1790 Size: 1031 1235 1156 2573 Fragments arranged by size: 2573 1235 1156 1031 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2271 2550 3044 4655 1239 Size: 912 120 279 494 1611 2579 Fragments arranged by size: 2579 1611 912 494 279 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4120 4985 5283 4120 Size: 865 298 4832 Fragments arranged by size: 4832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4658 882 Size: 617 3159 2219 Fragments arranged by size: 3159 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2093 2184 4428 4826 5100 5247 5658 2093 Size: 91 2244 398 274 147 411 2430 Fragments arranged by size: 2430 2244 411 398 274 147 91 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 1178 Size: 30 165 30 601 30 5139 Fragments arranged by size: 5139 601 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 1186 Size: 1198 4797 BseYI C'CCAG_C Cuts at: 5198 5694 5198 Size: 496 5499 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4056 4141 5212 4056 Size: 85 1071 4839 Fragments arranged by size: 4839 1071 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2821 3314 4056 4141 5212 1391 Size: 399 1031 493 742 85 1071 2174 Fragments arranged by size: 2174 1071 1031 742 493 399 85 BspEI T'CCGG_A Cuts at: 2093 2184 5658 2093 Size: 91 3474 2430 Fragments arranged by size: 3474 2430 91 BspHI T'CATG_A Cuts at: 2665 2856 3884 5614 2665 Size: 191 1028 1730 3046 Fragments arranged by size: 3046 1730 1028 191 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 844 Size: 2506 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 4490 4664 65 Size: 1183 1275 1967 174 1396 Fragments arranged by size: 1967 1396 1275 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4639 4826 1700 Size: 110 2829 187 2869 Fragments arranged by size: 2869 2829 187 110 BsrGI T'GTAC_A Cuts at: 96 3154 96 Size: 3058 2937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 2152 4799 2152 Size: 2647 3348 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2341 3325 3692 4077 4094 5546 5632 5644 Size: 369 984 367 385 17 1452 86 12 Cuts at: 5644 5990 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 984 385 369 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 2686 3163 513 Size: 552 939 442 240 477 3345 Fragments arranged by size: 3345 939 552 477 442 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2095 487 Size: 683 56 869 4387 Fragments arranged by size: 4387 869 683 56 BtsI GCAGTG_nn' Cuts at: 3093 3511 4305 4325 3093 Size: 418 794 20 4763 Fragments arranged by size: 4763 794 418 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2047 3442 Size: 54 1425 50 275 18 177 40 1395 Cuts at: 3442 4331 5960 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1395 889 275 177 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 3925 1353 Size: 1473 1099 3423 Fragments arranged by size: 3423 1473 1099 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3079 4582 4956 5102 387 Size: 1064 138 1490 1503 374 146 1280 Fragments arranged by size: 1503 1490 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2271 2550 3044 4058 4655 5442 Size: 393 519 120 279 494 1014 597 787 Cuts at: 5442 5710 1239 Size: 268 1524 Fragments arranged by size: 1524 1014 787 597 519 494 393 279 268 120 EcoO109I rG'GnC_Cy Cuts at: 2028 2028 Size: 5995 EcoRI G'AATT_C Cuts at: 2148 2915 3304 3682 2148 Size: 767 389 378 4461 Fragments arranged by size: 4461 767 389 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2315 2976 2315 Size: 661 5334 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 5142 1678 Size: 3464 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2212 2244 2333 2365 3267 Size: 32 135 32 640 32 89 32 902 Cuts at: 3267 3299 4636 4668 4710 4742 1373 Size: 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1337 902 640 135 89 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 2376 2958 3204 748 Size: 1628 582 246 3539 Fragments arranged by size: 3539 1628 582 246 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3195 3594 3195 Size: 399 5596 MluI A'CGCG_T Cuts at: 2035 5871 2035 Size: 3836 2159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5109 5293 1305 Size: 3804 184 2007 Fragments arranged by size: 3804 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2325 3025 3027 3953 4312 4471 518 Size: 648 1159 700 2 926 359 159 2042 Fragments arranged by size: 2042 1159 926 700 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2068 3064 3411 4088 5236 5481 1606 Size: 462 996 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 996 677 462 347 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2446 2686 3163 513 Size: 552 939 442 240 477 3345 Fragments arranged by size: 3345 939 552 477 442 240 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3184 3353 4898 1132 Size: 97 1955 169 1545 2229 Fragments arranged by size: 2229 1955 1545 169 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 3180 4894 3180 Size: 1714 4281 PflMI CCAn_nnn'nTGG Cuts at: 1108 3236 1108 Size: 2128 3867 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 2028 Size: 5995 PshAI GACnn'nnGTC Cuts at: 1613 3054 1613 Size: 1441 4554 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PstI C_TGCA'G Cuts at: 830 2494 3347 830 Size: 1664 853 3478 Fragments arranged by size: 3478 1664 853 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2068 3064 2068 Size: 996 4999 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SanDI GG'GwC_CC Cuts at: 2028 2028 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2490 2984 3343 4478 5159 5350 826 Size: 219 1445 494 359 1135 681 191 1471 Fragments arranged by size: 1471 1445 1135 681 494 359 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4099 5000 5262 820 Size: 197 926 2156 901 262 1553 Fragments arranged by size: 2156 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 2311 3113 3919 5 Size: 47 2259 802 806 2081 Fragments arranged by size: 2259 2081 806 802 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 2446 2686 3163 3434 513 Size: 552 939 442 240 477 271 3074 Fragments arranged by size: 3074 939 552 477 442 271 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4022 4039 4192 4377 4822 325 Size: 1255 2442 17 153 185 445 1498 Fragments arranged by size: 2442 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3154 4241 Size: 274 80 33 51 1443 369 808 1087 Cuts at: 4241 5953 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1087 808 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 2592 2624 5955 5987 2004 Size: 32 556 32 3331 32 2012 Fragments arranged by size: 3331 2012 556 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 2110 3331 2110 Size: 1221 4774 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3100 3100 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 2161 2204 2220 4124 2161 Size: 43 16 1904 4032 Fragments arranged by size: 4032 1904 43 16 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI