(Circular) (Six-base) MAPSORT of: pFN21AE0991.seq Check: 4954 from: 1 to: 6583 pFN21AE0991 6583 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3304 3304 Size: 6583 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3926 1937 Size: 280 1709 4594 Fragments arranged by size: 4594 1709 280 Acc65I G'GTAC_C Cuts at: 2496 3904 2496 Size: 1408 5175 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 3417 3657 3715 4646 6030 6298 1632 Size: 647 1138 240 58 931 1384 268 1917 Fragments arranged by size: 1917 1384 1138 931 647 268 240 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 2777 2935 3368 3498 4326 5898 2777 Size: 158 433 130 828 1572 3462 Fragments arranged by size: 3462 1572 828 433 158 130 ApaI G_GGCC'C Cuts at: 2036 2043 2073 3389 3616 2036 Size: 7 30 1316 227 5003 Fragments arranged by size: 5003 1316 227 30 7 ApaLI G'TGCA_C Cuts at: 2363 4640 5796 2363 Size: 2277 1156 3150 Fragments arranged by size: 3150 2277 1156 ApoI r'AATT_y Cuts at: 1493 3892 4118 4270 6379 1493 Size: 2399 226 152 2109 1697 Fragments arranged by size: 2399 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2074 2101 2484 2760 3132 3329 Size: 73 99 32 27 383 276 372 197 Cuts at: 3329 3630 3681 3908 1870 Size: 301 51 227 4545 Fragments arranged by size: 4545 383 372 301 276 227 197 99 73 51 32 27 BamHI G'GATC_C Cuts at: 2943 3913 4280 2943 Size: 970 367 5246 Fragments arranged by size: 5246 970 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2229 2328 2496 3831 Size: 325 217 627 269 173 99 168 1335 Cuts at: 3831 3904 5359 618 Size: 73 1455 1842 Fragments arranged by size: 1842 1455 1335 627 325 269 217 173 168 99 73 BanII G_rGCy'C Cuts at: 1391 2036 2043 2073 2134 2663 2754 3008 3336 Size: 645 7 30 61 529 91 254 328 Cuts at: 3336 3389 3603 3616 3690 3902 1391 Size: 53 214 13 74 212 4072 Fragments arranged by size: 4072 645 529 328 254 214 212 91 74 61 53 30 13 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 928 Size: 971 1266 4346 Fragments arranged by size: 4346 1266 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4774 4808 1903 Size: 34 2837 34 3678 Fragments arranged by size: 3678 2837 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4475 5685 4475 Size: 1210 5373 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 2458 5194 Size: 107 122 71 631 605 405 381 2736 Cuts at: 5194 136 Size: 1525 Fragments arranged by size: 2736 1525 631 605 405 381 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 2426 4000 2426 Size: 1574 5009 Bme1580I G_kGCm'C Cuts at: 1790 2036 2043 2073 2090 2367 2911 3389 3616 Size: 246 7 30 17 277 544 478 227 Cuts at: 3616 3758 3834 3877 4644 5800 1790 Size: 142 76 43 767 1156 2573 Fragments arranged by size: 2573 1156 767 544 478 277 246 227 142 76 43 30 17 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 3124 4314 Size: 416 53 83 399 24 854 853 1190 Cuts at: 4314 5272 442 Size: 958 1753 Fragments arranged by size: 1753 1190 958 854 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2070 2664 2969 3426 3665 5243 1239 Size: 831 594 305 457 239 1578 2579 Fragments arranged by size: 2579 1578 831 594 457 305 239 Bpu10I CC'TnA_GC Cuts at: 2324 2961 3429 2324 Size: 637 468 5478 Fragments arranged by size: 5478 637 468 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3266 5246 882 Size: 617 1767 1980 2219 Fragments arranged by size: 2219 1980 1767 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4390 4772 5407 275 Size: 53 83 186 936 2857 382 635 1451 Fragments arranged by size: 2857 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3268 5016 5414 5688 5835 6246 3268 Size: 1748 398 274 147 411 3605 Fragments arranged by size: 3605 1748 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2346 2376 2489 2519 1178 Size: 30 165 30 943 30 113 30 5242 Fragments arranged by size: 5242 943 165 113 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 3806 1186 Size: 924 1696 3963 Fragments arranged by size: 3963 1696 924 BseYI C'CCAG_C Cuts at: 2897 2928 3197 3456 3559 3588 3593 3615 3854 Size: 31 269 259 103 29 5 22 239 Cuts at: 3854 5786 6282 2897 Size: 1932 496 3198 Fragments arranged by size: 3198 1932 496 269 259 239 103 31 29 22 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2363 2486 1163 Size: 573 591 36 123 5260 Fragments arranged by size: 5260 591 573 123 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 2754 3336 4644 4729 5800 2367 Size: 387 582 1308 85 1071 3150 Fragments arranged by size: 3150 1308 1071 582 387 85 BsmI GAATG_Cn' Cuts at: 4094 4187 4094 Size: 93 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2043 2073 2090 2134 2367 2663 Size: 399 246 7 30 17 44 233 296 Cuts at: 2663 2754 2911 3008 3336 3389 3603 3616 3690 Size: 91 157 97 328 53 214 13 74 Cuts at: 3690 3758 3834 3877 3902 4644 4729 5800 1391 Size: 68 76 43 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 296 246 233 214 157 97 91 85 76 74 68 53 44 43 30 25 17 13 7 BspEI T'CCGG_A Cuts at: 3268 6246 3268 Size: 2978 3605 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 2776 3304 3938 844 Size: 1664 268 528 634 3489 Fragments arranged by size: 3489 1664 634 528 268 BsrBI CCG'CTC Cuts at: 1394 1823 3312 3649 4470 1394 Size: 429 1489 337 821 3507 Fragments arranged by size: 3507 1489 821 429 337 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 5078 5252 65 Size: 1183 1230 2600 174 1396 Fragments arranged by size: 2600 1396 1230 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 2319 2452 3069 5227 5414 1700 Size: 110 388 121 133 617 2158 187 2869 Fragments arranged by size: 2869 2158 617 388 187 133 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 3625 794 Size: 2831 3752 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 3259 1184 Size: 2075 4508 BstXI CCAn_nnnn'nTGG Cuts at: 3588 3588 Size: 6583 BstYI r'GATC_y Cuts at: 1972 2943 3049 3913 4280 4665 4682 6134 6220 Size: 971 106 864 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 971 864 385 367 346 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2342 3164 2342 Size: 822 5761 BtgI C'CryG_G Cuts at: 513 1065 2004 2652 513 Size: 552 939 648 4444 Fragments arranged by size: 4444 939 648 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2740 487 Size: 683 56 1514 4330 Fragments arranged by size: 4330 1514 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2176 2852 2878 3356 4099 4893 4913 2024 Size: 152 676 26 478 743 794 20 3694 Fragments arranged by size: 3694 794 743 676 478 152 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2450 2601 Size: 54 1425 50 275 18 177 443 151 Cuts at: 2601 2649 2871 3474 4030 4919 6548 8 Size: 48 222 603 556 889 1629 43 Fragments arranged by size: 1629 1425 889 603 556 443 275 222 177 151 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3086 3393 4513 1353 Size: 1733 307 1120 3423 Fragments arranged by size: 3423 1733 1120 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 5170 5544 5690 387 Size: 1064 138 520 3061 374 146 1280 Fragments arranged by size: 3061 1280 1064 520 374 146 138 EcoICRI GAG'CTC Cuts at: 2752 3334 2752 Size: 582 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2279 2664 2969 3417 3426 3657 Size: 393 438 209 385 305 448 9 231 Cuts at: 3657 3665 3715 4646 5243 6030 6298 1239 Size: 8 50 931 597 787 268 1524 Fragments arranged by size: 1524 931 787 597 448 438 393 385 305 268 231 209 50 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 3531 3531 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 2032 2033 2069 2070 3023 3365 3386 3536 2032 Size: 1 36 1 953 342 21 150 5079 Fragments arranged by size: 5079 953 342 150 36 21 1 1 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2145 3455 5730 1678 Size: 467 1310 2275 2531 Fragments arranged by size: 2531 2275 1310 467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2551 2983 3015 3528 Size: 32 135 32 947 32 432 32 513 Cuts at: 3528 3560 5224 5256 5298 5330 1373 Size: 32 1664 32 42 32 2626 Fragments arranged by size: 2626 1664 947 513 432 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3139 3927 4173 669 Size: 988 281 1201 788 246 3079 Fragments arranged by size: 3079 1201 988 788 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 2500 3908 2500 Size: 1408 5175 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 5697 5881 1305 Size: 1273 3119 184 2007 Fragments arranged by size: 3119 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2873 3476 10 Size: 54 1425 343 1041 603 3117 Fragments arranged by size: 3117 1425 1041 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4541 4900 5059 518 Size: 648 3375 359 159 2042 Fragments arranged by size: 3375 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 2377 2422 2579 2956 3250 3306 3380 Size: 705 66 45 157 377 294 56 74 Cuts at: 3380 3460 3563 3773 3865 3999 4676 5824 6069 Size: 80 103 210 92 134 677 1148 245 Cuts at: 6069 1606 Size: 2120 Fragments arranged by size: 2120 1148 705 677 377 294 245 210 157 134 103 92 80 74 66 56 45 NaeI GCC'GGC Cuts at: 2200 2200 Size: 6583 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5092 Fragments arranged by size: 5092 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NgoMIV G'CCGG_C Cuts at: 2198 2198 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3941 5486 1132 Size: 97 2712 1545 2229 Fragments arranged by size: 2712 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2036 1348 Size: 688 5895 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 2935 3368 1108 Size: 1827 433 4323 Fragments arranged by size: 4323 1827 433 PfoI T'CCnGG_A Cuts at: 2946 2946 Size: 6583 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2409 2441 4652 4684 6165 6197 2409 Size: 32 2211 32 1481 32 2795 Fragments arranged by size: 2795 2211 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3023 3365 3536 3023 Size: 342 171 6070 Fragments arranged by size: 6070 342 171 PshAI GACnn'nnGTC Cuts at: 1613 3134 3278 1613 Size: 1521 144 4918 Fragments arranged by size: 4918 1521 144 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PspOMI G'GGCC_C Cuts at: 2032 2039 2069 3385 3612 2032 Size: 7 30 1316 227 5003 Fragments arranged by size: 5003 1316 227 30 7 PspXI vC'TCGA_Gb Cuts at: 3132 3132 Size: 6583 PstI C_TGCA'G Cuts at: 830 2773 3864 3935 830 Size: 1943 1091 71 3478 Fragments arranged by size: 3478 1943 1091 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2311 2377 2422 2956 3250 3460 3563 3773 3865 Size: 66 45 534 294 210 103 210 92 Cuts at: 3865 2311 Size: 5029 Fragments arranged by size: 5029 534 294 210 210 103 92 66 45 SacI G_AGCT'C Cuts at: 2754 3336 2754 Size: 582 6001 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SanDI GG'GwC_CC Cuts at: 3023 3023 Size: 6583 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SfcI C'TryA_G Cuts at: 826 1045 2769 3860 3931 5066 5747 5938 826 Size: 219 1724 1091 71 1135 681 191 1471 Fragments arranged by size: 1724 1471 1135 1091 681 219 191 71 SfiI GGCCn_nnn'nGGCC Cuts at: 2077 2077 Size: 6583 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 2044 2076 2103 3632 3683 3910 1872 Size: 172 32 27 1529 51 227 4545 Fragments arranged by size: 4545 1529 227 172 51 32 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 3132 4687 5588 5850 820 Size: 197 926 1189 1555 901 262 1553 Fragments arranged by size: 1555 1553 1189 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SrfI GCCC'GGGC Cuts at: 2044 2103 2044 Size: 59 6524 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 2490 1517 Size: 973 5610 StyI C'CwwG_G Cuts at: 513 1065 2004 3255 4022 513 Size: 552 939 1251 767 3074 Fragments arranged by size: 3074 1251 939 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 3211 3862 3868 4610 4627 4780 Size: 1255 996 635 651 6 742 17 153 Cuts at: 4780 4965 5410 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 996 742 651 635 445 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4829 6541 96 Size: 274 80 33 51 1443 2852 1712 138 Fragments arranged by size: 2852 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 6543 6575 2346 Size: 32 4165 32 2354 Fragments arranged by size: 4165 2354 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 3132 1943 Size: 1189 5394 XmaI C'CCGG_G Cuts at: 1870 2042 2074 2101 3630 3681 3908 1870 Size: 172 32 27 1529 51 227 4545 Fragments arranged by size: 4545 1529 227 172 51 32 27 XmnI GAAnn'nnTTC Cuts at: 2610 4712 2610 Size: 2102 4481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SapI SexAI SfoI SwaI XcmI