(Linear) (Six-base) MAPSORT of: KIEE0991.seq Check: 371 from: 1 to: 1878 KIEE0991.orf With 182 enzymes: SgfI * July 3, 2012 16:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1299 1878 Size: 1299 579 AccI GT'mk_AC Cuts at: 0 212 1878 Size: 212 1666 Acc65I G'GTAC_C Cuts at: 0 491 1878 Size: 491 1387 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 1412 1652 1710 1878 Size: 274 1138 240 58 168 Fragments arranged by size: 1138 274 240 168 58 AlwNI CAG_nnn'CTG Cuts at: 0 772 930 1363 1493 1878 Size: 772 158 433 130 385 Fragments arranged by size: 772 433 385 158 130 ApaI G_GGCC'C Cuts at: 0 31 38 68 1384 1611 1878 Size: 31 7 30 1316 227 267 Fragments arranged by size: 1316 267 227 31 30 7 ApaLI G'TGCA_C Cuts at: 0 358 1878 Size: 358 1520 AvaI C'yCGr_G Cuts at: 0 37 69 96 479 755 1127 1324 1625 Size: 37 32 27 383 276 372 197 301 Cuts at: 1625 1676 1878 Size: 51 202 Fragments arranged by size: 383 372 301 276 202 197 51 37 32 27 BamHI G'GATC_C Cuts at: 0 938 1878 Size: 938 940 BanI G'GyrC_C Cuts at: 0 51 224 323 491 1826 1878 Size: 51 173 99 168 1335 52 Fragments arranged by size: 1335 173 168 99 52 51 BanII G_rGCy'C Cuts at: 0 31 38 68 129 658 749 1003 1331 Size: 31 7 30 61 529 91 254 328 Cuts at: 1331 1384 1598 1611 1685 1878 Size: 53 214 13 74 193 Fragments arranged by size: 529 328 254 214 193 91 74 61 53 31 30 13 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 1160 1878 Size: 1160 718 BglI GCCn_nnn'nGGC Cuts at: 0 72 453 1878 Size: 72 381 1425 Fragments arranged by size: 1425 381 72 BlpI GC'TnA_GC Cuts at: 0 421 1878 Size: 421 1457 Bme1580I G_kGCm'C Cuts at: 0 31 38 68 85 362 906 1384 1611 Size: 31 7 30 17 277 544 478 227 Cuts at: 1611 1753 1829 1872 1878 Size: 142 76 43 6 Fragments arranged by size: 544 478 277 227 142 76 43 31 30 17 7 6 BmrI ACTGGGnnnn_n' Cuts at: 0 266 1119 1878 Size: 266 853 759 Fragments arranged by size: 853 759 266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 659 964 1421 1660 1878 Size: 65 594 305 457 239 218 Fragments arranged by size: 594 457 305 239 218 65 Bpu10I CC'TnA_GC Cuts at: 0 319 956 1424 1878 Size: 319 637 468 454 Fragments arranged by size: 637 468 454 319 BsaI GGTCTCn'nnnn_ Cuts at: 0 1261 1878 Size: 1261 617 BsaWI w'CCGG_w Cuts at: 0 1263 1878 Size: 1263 615 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 341 371 484 514 1878 Size: 341 30 113 30 1364 Fragments arranged by size: 1364 341 113 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 105 1801 1878 Size: 105 1696 77 Fragments arranged by size: 1696 105 77 BseYI C'CCAG_C Cuts at: 0 892 923 1192 1451 1554 1583 1588 1610 Size: 892 31 269 259 103 29 5 22 Cuts at: 1610 1849 1878 Size: 239 29 Fragments arranged by size: 892 269 259 239 103 31 29 29 22 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 358 481 1878 Size: 322 36 123 1397 Fragments arranged by size: 1397 322 123 36 BsiHKAI G_wGCw'C Cuts at: 0 362 749 1331 1878 Size: 362 387 582 547 Fragments arranged by size: 582 547 387 362 Bsp1286I G_dGCh'C Cuts at: 0 31 38 68 85 129 362 658 749 Size: 31 7 30 17 44 233 296 91 Cuts at: 749 906 1003 1331 1384 1598 1611 1685 1753 Size: 157 97 328 53 214 13 74 68 Cuts at: 1753 1829 1872 1878 Size: 76 43 6 Fragments arranged by size: 328 296 233 214 157 97 91 76 74 68 53 44 43 31 30 17 13 7 6 BspEI T'CCGG_A Cuts at: 0 1263 1878 Size: 1263 615 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 503 771 1299 1878 Size: 503 268 528 579 Fragments arranged by size: 579 528 503 268 BsrBI CCG'CTC Cuts at: 0 1307 1644 1878 Size: 1307 337 234 Fragments arranged by size: 1307 337 234 BsrDI GCAATG_nn' Cuts at: 0 473 1878 Size: 473 1405 BsrFI r'CCGG_y Cuts at: 0 193 314 447 1064 1878 Size: 193 121 133 617 814 Fragments arranged by size: 814 617 193 133 121 BstAPI GCAn_nnn'nTGC Cuts at: 0 1620 1878 Size: 1620 258 BstEII G'GTnAC_C Cuts at: 0 1254 1878 Size: 1254 624 BstXI CCAn_nnnn'nTGG Cuts at: 0 1583 1878 Size: 1583 295 BstYI r'GATC_y Cuts at: 0 938 1044 1878 Size: 938 106 834 Fragments arranged by size: 938 834 106 Bsu36I CC'TnA_GG Cuts at: 0 337 1159 1878 Size: 337 822 719 Fragments arranged by size: 822 719 337 BtgI C'CryG_G Cuts at: 0 647 1878 Size: 647 1231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 735 1878 Size: 735 1143 BtsI GCAGTG_nn' Cuts at: 0 19 171 847 873 1351 1878 Size: 19 152 676 26 478 527 Fragments arranged by size: 676 527 478 152 26 19 EaeI y'GGCC_r Cuts at: 0 2 445 596 644 866 1469 1878 Size: 2 443 151 48 222 603 409 Fragments arranged by size: 603 443 409 222 151 48 2 EarI CTCTTCn'nnn_ Cuts at: 0 1081 1388 1878 Size: 1081 307 490 Fragments arranged by size: 1081 490 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 104 1878 Size: 104 1774 EcoICRI GAG'CTC Cuts at: 0 747 1329 1878 Size: 747 582 549 Fragments arranged by size: 747 582 549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 274 659 964 1412 1421 1652 1660 Size: 65 209 385 305 448 9 231 8 Cuts at: 1660 1710 1878 Size: 50 168 Fragments arranged by size: 448 385 305 231 209 168 65 50 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 1526 1878 Size: 1526 352 EcoO109I rG'GnC_Cy Cuts at: 0 27 28 64 65 1018 1360 1381 1531 Size: 27 1 36 1 953 342 21 150 Cuts at: 1531 1878 Size: 347 Fragments arranged by size: 953 347 342 150 36 27 21 1 1 HaeII r_GCGC'y Cuts at: 0 140 1450 1878 Size: 140 1310 428 Fragments arranged by size: 1310 428 140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 514 546 978 1010 1523 1555 1878 Size: 514 32 432 32 513 32 323 Fragments arranged by size: 514 513 432 323 32 32 32 HincII GTy'rAC Cuts at: 0 1134 1878 Size: 1134 744 KpnI G_GTAC'C Cuts at: 0 495 1878 Size: 495 1383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 573 1878 Size: 573 1305 MscI TGG'CCA Cuts at: 0 868 1471 1878 Size: 868 603 407 Fragments arranged by size: 868 603 407 MspA1I CmG'CkG Cuts at: 0 306 372 417 574 951 1245 1301 1375 Size: 306 66 45 157 377 294 56 74 Cuts at: 1375 1455 1558 1768 1860 1878 Size: 80 103 210 92 18 Fragments arranged by size: 377 306 294 210 157 103 92 80 74 66 56 45 18 NaeI GCC'GGC Cuts at: 0 195 1878 Size: 195 1683 NgoMIV G'CCGG_C Cuts at: 0 193 1878 Size: 193 1685 PasI CC'CwG_GG Cuts at: 0 31 1878 Size: 31 1847 PflMI CCAn_nnn'nTGG Cuts at: 0 930 1363 1878 Size: 930 433 515 Fragments arranged by size: 930 515 433 PfoI T'CCnGG_A Cuts at: 0 941 1878 Size: 941 937 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 404 436 1878 Size: 404 32 1442 Fragments arranged by size: 1442 404 32 PpuMI rG'GwC_Cy Cuts at: 0 1018 1360 1531 1878 Size: 1018 342 171 347 Fragments arranged by size: 1018 347 342 171 PshAI GACnn'nnGTC Cuts at: 0 1129 1273 1878 Size: 1129 144 605 Fragments arranged by size: 1129 605 144 PspOMI G'GGCC_C Cuts at: 0 27 34 64 1380 1607 1878 Size: 27 7 30 1316 227 271 Fragments arranged by size: 1316 271 227 30 27 7 PspXI vC'TCGA_Gb Cuts at: 0 1127 1878 Size: 1127 751 PstI C_TGCA'G Cuts at: 0 768 1859 1878 Size: 768 1091 19 Fragments arranged by size: 1091 768 19 PvuII CAG'CTG Cuts at: 0 306 372 417 951 1245 1455 1558 1768 Size: 306 66 45 534 294 210 103 210 Cuts at: 1768 1860 1878 Size: 92 18 Fragments arranged by size: 534 306 294 210 210 103 92 66 45 18 SacI G_AGCT'C Cuts at: 0 749 1331 1878 Size: 749 582 547 Fragments arranged by size: 749 582 547 SanDI GG'GwC_CC Cuts at: 0 1018 1878 Size: 1018 860 SfcI C'TryA_G Cuts at: 0 764 1855 1878 Size: 764 1091 23 Fragments arranged by size: 1091 764 23 SfiI GGCCn_nnn'nGGCC Cuts at: 0 72 1878 Size: 72 1806 SmaI CCC'GGG Cuts at: 0 39 71 98 1627 1678 1878 Size: 39 32 27 1529 51 200 Fragments arranged by size: 1529 200 51 39 32 27 SmlI C'TyrA_G Cuts at: 0 1127 1878 Size: 1127 751 SrfI GCCC'GGGC Cuts at: 0 39 98 1878 Size: 39 59 1780 Fragments arranged by size: 1780 59 39 StuI AGG'CCT Cuts at: 0 485 1878 Size: 485 1393 StyI C'CwwG_G Cuts at: 0 1250 1878 Size: 1250 628 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 571 1206 1857 1863 1878 Size: 571 635 651 6 15 Fragments arranged by size: 651 635 571 15 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 341 373 1878 Size: 341 32 1505 Fragments arranged by size: 1505 341 32 XhoI C'TCGA_G Cuts at: 0 1127 1878 Size: 1127 751 XmaI C'CCGG_G Cuts at: 0 37 69 96 1625 1676 1878 Size: 37 32 27 1529 51 202 Fragments arranged by size: 1529 202 51 37 32 27 XmnI GAAnn'nnTTC Cuts at: 0 605 1878 Size: 605 1273 Enzymes that do cut: AarI AccI Acc65I AcuI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MspA1I NaeI NgoMIV PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TstI XhoI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XcmI ZraI