Restriction map for
pFN21AE4355
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI HinP1I HpaI KasI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 220 GACGTC GACGT^C
Acc65I 307 GGTACC G^GTACC
AcsI 133 GAATTT [GA]^AATT[CT]
AcyI 220 GACGTC G[GA]^CG[CT]C
AlwNI 313 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 133 GAATTT [GA]^AATT[CT]
Asp718I 307 GGTACC G^GTACC
BamHI 626 GGATCC G^GATCC
BanI 307 GGTACC G^G[CT][GA]CC
BbsI 352 GTCTTC GAAGAC(2/6)
BbvI 11 GCTGC GCAGC(8/12)
BcnI 383 CCGGG CC^[GC]GG
BglI 703 GCCTTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1005 AGATCT A^GATCT
BlpI 997 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 997 GCTCAGC GC^T[ACGT]AGC
BpuAI 352 GTCTTC GAAGAC(2/6)
BsaHI 220 GACGTC G[GA]^CG[CT]C
BsaI 1093 GAGACC GGTCTC(1/5)
BsiEI 227 CGGTCG CG[GA][CT]^CG
BsmBI 222 CGTCTC CGTCTC(1/5)
BsmFI 719 GGGAC GGGAC(10/14)
Bsp1407I 536 TGTACA T^GTACA
BspHI 287 TCATGA T^CATGA
BsrFI 1082 ACCGGC [GA]^CCGG[CT]
BsrGI 536 TGTACA T^GTACA
CelII 997 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1082 ACCGGC [GA]^CCGG[CT]
DsaI 1055 CCGTGG C^C[GA][CT]GG
EaeI 417 CGGCCA [CT]^GGCC[GA]
EcoT14I 98 CCTTGG C^C[AT][AT]GG
FauI 717 GCGGG CCCGC(4/6)
Hin1I 220 GACGTC G[GA]^CG[CT]C
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
HindIII 475 AAGCTT A^AGCTT
KpnI 307 GGTACC GGTAC^C
MspA1I 1029 CAGCTG C[AC]G^C[GT]G
NciI 383 CCGGG CC^[GC]GG
PflMI 693 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 719 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1029 CAGCTG CAG^CTG
RcaI 287 TCATGA T^CATGA
SapI 427 GAAGAGC GCTCTTC(1/4)
SmlI 705 CTTGAG C^T[CT][GA]AG
SspBI 536 TGTACA T^GTACA
StyI 98 CCTTGG C^C[AT][AT]GG
TseI 11 GCTGC G^C[AT]GC
TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 693 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1021 CCACTCTACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 385 GGGCCC GGGCC^C
689 GGGCCC
BpmI 145 CTCCAG CTGGAG(16/14)
6 CTGGAG
BstNI 216 CCAGG CC^[AT]GG
686 CCTGG
BstYI 626 GGATCC [GA]^GATC[CT]
1005 AGATCT
DrdI 220 GACGTCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1095 GACCCTGAAGTC
EarI 70 GAAGAG CTCTTC(1/4)
427 GAAGAG
EcoRII 216 CCAGG ^CC[AT]GG
686 CCTGG
Fnu4HI 11 GCTGC GC^[ACGT]GC
499 GCGGC
HapII 383 CCGG C^CGG
1083 CCGG
HpaII 383 CCGG C^CGG
1083 CCGG
ItaI 11 GCTGC GC^[ACGT]GC
499 GCGGC
Ksp632I 70 GAAGAG CTCTTC(1/4)
427 GAAGAG
MaeII 221 ACGT A^CGT
680 ACGT
MflI 626 GGATCC [GA]^GATC[CT]
1005 AGATCT
MspI 383 CCGG C^CGG
1083 CCGG
MvaI 216 CCAGG CC^[AT]GG
686 CCTGG
NlaIII 288 CATG CATG^
446 CATG
PleI 1110 GACTC GAGTC(4/5)
280 GAGTC
SfcI 653 CTACAG C^T[GA][CT]AG
1026 CTACAG
StuI 107 AGGCCT AGG^CCT
326 AGGCCT
TfiI 513 GAATC G^A[AT]TC
838 GATTC
XhoII 626 GGATCC [GA]^GATC[CT]
1005 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I FokI HaeIII HinfI HphI
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI Tru9I Tsp45I Tsp509I