Restriction map for
pFN21AE4355
(Circular) (Six-base) MAPSORT of: pFN21AE4355.seq Check: 4701 from: 1 to: 5821
pFN21AE4355 5821 bp
With 182 enzymes: SgfI *
March 28, 2012 13:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2229 3631 278
Size: 53 83 186 936 693 1402 2468
Fragments arranged by size:
2468 1402 936 693 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 2312 3142 2312
Size: 830 4991
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2101 2108 3125 3884 5268 5536 1632
Size: 469 7 1017 759 1384 268 1917
Fragments arranged by size:
1917 1384 1017 759 469 268 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 2323 3564 5136 2323
Size: 1241 1572 3008
Fragments arranged by size:
3008 1572 1241
ApaI G_GGCC'C
Cuts at: 2394 2698 2394
Size: 304 5517
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2138 3130 3356 3508 5617 1493
Size: 645 992 226 152 2109 1697
Fragments arranged by size:
2109 1697 992 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 2631 3151 3518 2631
Size: 520 367 4934
Fragments arranged by size:
4934 520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 3142 4597 618
Size: 325 217 627 525 830 1455 1842
Fragments arranged by size:
1842 1455 830 627 525 325 217
BanII G_rGCy'C
Cuts at: 1391 2394 2698 2719 3140 1391
Size: 1003 304 21 421 4072
Fragments arranged by size:
4072 1003 421 304 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 928
Size: 971 451 4399
Fragments arranged by size:
4399 971 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 4432 136
Size: 107 122 71 631 605 1042 1718 1525
Fragments arranged by size:
1718 1525 1042 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3010 5816 3010
Size: 2806 3015
BlpI GC'TnA_GC
Cuts at: 3003 3238 3003
Size: 235 5586
Bme1580I G_kGCm'C
Cuts at: 1790 2394 2698 3882 5038 1790
Size: 604 304 1184 1156 2573
Fragments arranged by size:
2573 1184 1156 604 304
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2663 3552 4510
Size: 416 53 83 399 24 1246 889 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1246 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2135 4481 1239
Size: 793 103 2346 2579
Fragments arranged by size:
2579 2346 793 103
Bpu10I CC'TnA_GC
Cuts at: 2816 2816
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2731 3946 4811 5109 2731
Size: 1215 865 298 3443
Fragments arranged by size:
3443 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3092 4484 882
Size: 617 1593 1392 2219
Fragments arranged by size:
2219 1593 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 3628 4010 4645
Size: 53 83 186 936 693 1402 382 635
Cuts at: 4645 275
Size: 1451
Fragments arranged by size:
1451 1402 936 693 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2316 3036 5024 5520 2316
Size: 720 1988 496 2617
Fragments arranged by size:
2617 1988 720 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 3271 4032 5060 1540
Size: 275 186 234 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
BsmBI CGTCTCn'nnnn_
Cuts at: 2233 2233
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2698 2719 3140 3882 3967 5038
Size: 399 604 304 21 421 742 85 1071
Cuts at: 5038 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 604 421 399 304 85
21
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 2292 3710 5440 2292
Size: 1418 1730 2673
Fragments arranged by size:
2673 1730 1418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3176 844
Size: 2332 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3087 4465 4652 1700
Size: 110 1277 1378 187 2869
Fragments arranged by size:
2869 1378 1277 187 110
BsrGI T'GTAC_A
Cuts at: 96 2541 96
Size: 2445 3376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2631 3010 3151 3518 3903 3920 5372 5458
Size: 659 379 141 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 659 385 379 367 346 141
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3060 513
Size: 552 939 1056 3274
Fragments arranged by size:
3274 1056 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2231 3106 4828 809
Size: 507 915 875 1722 1802
Fragments arranged by size:
1802 1722 915 875 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2422 3268 4157
Size: 54 1425 50 275 18 592 846 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 846 592 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2427 3751 1353
Size: 717 357 1324 3423
Fragments arranged by size:
3423 1324 717 357
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2825 4408 4782 4928 387
Size: 1064 138 462 774 1583 374 146 1280
Fragments arranged by size:
1583 1280 1064 774 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2101 2108 2135 3125 3884 4481
Size: 393 400 69 7 27 990 759 597
Cuts at: 4481 5268 5536 1239
Size: 787 268 1524
Fragments arranged by size:
1524 990 787 759 597 400 393 268
69 27 7
EcoO109I rG'GnC_Cy
Cuts at: 2100 2614 2725 2737 2100
Size: 514 111 12 5184
Fragments arranged by size:
5184 514 111 12
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 4462 4494 4536
Size: 32 135 32 702 32 2156 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 2156 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 3165 3411 669
Size: 988 281 727 500 246 3079
Fragments arranged by size:
3079 988 727 500 281 246
HindIII A'AGCT_T
Cuts at: 748 2480 748
Size: 1732 4089
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 2316 3146 2316
Size: 830 4991
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 2626 4935 5119 1305
Size: 1002 319 2309 184 2007
Fragments arranged by size:
2309 2007 1002 319 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4138 4297 518
Size: 648 2613 359 159 2042
Fragments arranged by size:
2613 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3237 3914 5062 5307 1606
Size: 1430 201 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245 201
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2704 1108
Size: 902 694 4225
Fragments arranged by size:
4225 902 694
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2390 2694 2390
Size: 304 5517
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 3036 3036
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SanDI GG'GwC_CC
Cuts at: 2725 2725
Size: 5821
SapI GCTCTTCn'nnn_
Cuts at: 2427 2427
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2658 3031 3169 4304 4985 5176 826
Size: 219 1613 373 138 1135 681 191 1471
Fragments arranged by size:
1613 1471 1135 681 373 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2710 3925 4826 5088 820
Size: 197 926 767 1215 901 262 1553
Fragments arranged by size:
1553 1215 926 901 767 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2114 2333 1517
Size: 597 219 5005
Fragments arranged by size:
5005 597 219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 3260 513
Size: 552 939 99 1157 3074
Fragments arranged by size:
3074 1157 939 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 2502 3848 3865 4018 4203 4648
Size: 1255 641 281 1346 17 153 185 445
Cuts at: 4648 325
Size: 1498
Fragments arranged by size:
1498 1346 1255 641 445 281 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2173 2541 2874
Size: 274 80 33 51 1443 196 368 333
Cuts at: 2874 4067 5779 96
Size: 1193 1712 138
Fragments arranged by size:
1712 1443 1193 368 333 274 196 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 5781 5813 2270
Size: 32 3479 32 2278
Fragments arranged by size:
3479 2278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3033 3033
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2227 3629 276
Size: 53 83 186 936 693 1402 2468
Fragments arranged by size:
2468 1402 936 693 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI