Restriction map for
pFN21AE4355
(Linear) (Six-base) MAPSORT of: KIEE4355.seq Check: 9164 from: 1 to: 1116
KIEE4355.orf
With 182 enzymes: SgfI *
March 22, 2012 17:48 ..
AatII G_ACGT'C
Cuts at: 0 224 1116
Size: 224 892
Acc65I G'GTAC_C
Cuts at: 0 307 1116
Size: 307 809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 103 1116
Size: 96 7 1013
Fragments arranged by size:
1013 96 7
AlwNI CAG_nnn'CTG
Cuts at: 0 318 1116
Size: 318 798
ApaI G_GGCC'C
Cuts at: 0 389 693 1116
Size: 389 304 423
Fragments arranged by size:
423 389 304
ApoI r'AATT_y
Cuts at: 0 133 1116
Size: 133 983
BamHI G'GATC_C
Cuts at: 0 626 1116
Size: 626 490
BanI G'GyrC_C
Cuts at: 0 307 1116
Size: 307 809
BanII G_rGCy'C
Cuts at: 0 389 693 714 1116
Size: 389 304 21 402
Fragments arranged by size:
402 389 304 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 1116
Size: 345 771
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1116
Size: 709 407
BglII A'GATC_T
Cuts at: 0 1005 1116
Size: 1005 111
BlpI GC'TnA_GC
Cuts at: 0 998 1116
Size: 998 118
Bme1580I G_kGCm'C
Cuts at: 0 389 693 1116
Size: 389 304 423
Fragments arranged by size:
423 389 304
BmrI ACTGGGnnnn_n'
Cuts at: 0 658 1116
Size: 658 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 130 1116
Size: 27 103 986
Fragments arranged by size:
986 103 27
Bpu10I CC'TnA_GC
Cuts at: 0 811 1116
Size: 811 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1116
Size: 726 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 1087 1116
Size: 1087 29
BsaHI Gr'CG_yC
Cuts at: 0 221 1116
Size: 221 895
BseYI C'CCAG_C
Cuts at: 0 311 1031 1116
Size: 311 720 85
Fragments arranged by size:
720 311 85
BsiEI CG_ry'CG
Cuts at: 0 230 1116
Size: 230 886
BsmBI CGTCTCn'nnnn_
Cuts at: 0 228 1116
Size: 228 888
Bsp1286I G_dGCh'C
Cuts at: 0 389 693 714 1116
Size: 389 304 21 402
Fragments arranged by size:
402 389 304 21
BspHI T'CATG_A
Cuts at: 0 287 1116
Size: 287 829
BsrFI r'CCGG_y
Cuts at: 0 1082 1116
Size: 1082 34
BsrGI T'GTAC_A
Cuts at: 0 536 1116
Size: 536 580
BstYI r'GATC_y
Cuts at: 0 626 1005 1116
Size: 626 379 111
Fragments arranged by size:
626 379 111
BtgI C'CryG_G
Cuts at: 0 1055 1116
Size: 1055 61
DrdI GACnn_nn'nnGTC
Cuts at: 0 226 1101 1116
Size: 226 875 15
Fragments arranged by size:
875 226 15
EaeI y'GGCC_r
Cuts at: 0 417 1116
Size: 417 699
EarI CTCTTCn'nnn_
Cuts at: 0 65 422 1116
Size: 65 357 694
Fragments arranged by size:
694 357 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 820 1116
Size: 46 774 296
Fragments arranged by size:
774 296 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 96 103 130 1116
Size: 27 69 7 27 986
Fragments arranged by size:
986 69 27 27 7
EcoO109I rG'GnC_Cy
Cuts at: 0 95 609 720 732 1116
Size: 95 514 111 12 384
Fragments arranged by size:
514 384 111 95 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 1116
Size: 269 32 815
Fragments arranged by size:
815 269 32
HincII GTy'rAC
Cuts at: 0 660 1116
Size: 660 456
HindIII A'AGCT_T
Cuts at: 0 475 1116
Size: 475 641
KpnI G_GTAC'C
Cuts at: 0 311 1116
Size: 311 805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 621 1116
Size: 302 319 495
Fragments arranged by size:
495 319 302
MspA1I CmG'CkG
Cuts at: 0 1031 1116
Size: 1031 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1116
Size: 699 417
PpuMI rG'GwC_Cy
Cuts at: 0 720 1116
Size: 720 396
PspOMI G'GGCC_C
Cuts at: 0 385 689 1116
Size: 385 304 427
Fragments arranged by size:
427 385 304
PvuII CAG'CTG
Cuts at: 0 1031 1116
Size: 1031 85
SanDI GG'GwC_CC
Cuts at: 0 720 1116
Size: 720 396
SapI GCTCTTCn'nnn_
Cuts at: 0 422 1116
Size: 422 694
SfcI C'TryA_G
Cuts at: 0 653 1026 1116
Size: 653 373 90
Fragments arranged by size:
653 373 90
SmlI C'TyrA_G
Cuts at: 0 705 1116
Size: 705 411
StuI AGG'CCT
Cuts at: 0 109 328 1116
Size: 109 219 788
Fragments arranged by size:
788 219 109
StyI C'CwwG_G
Cuts at: 0 98 1116
Size: 98 1018
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 216 497 1116
Size: 216 281 619
Fragments arranged by size:
619 281 216
TatI w'GTAC_w
Cuts at: 0 168 536 869 1116
Size: 168 368 333 247
Fragments arranged by size:
368 333 247 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1116
Size: 265 32 819
Fragments arranged by size:
819 265 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1028 1116
Size: 1028 88
ZraI GAC'GTC
Cuts at: 0 222 1116
Size: 222 894
Enzymes that do cut:
AatII Acc65I AcuI AlwNI ApaI ApoI BamHI BanI
BanII BbsI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BseYI BsiEI BsmBI Bsp1286I
BspHI BsrFI BsrGI BstYI BtgI DrdI EaeI EarI
EciI Eco57MI EcoO109I Hin4I HincII HindIII KpnI MmeI
MspA1I PflMI PpuMI PspOMI PvuII SanDI SapI SfcI
SmlI StuI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsiWI BsmI BspEI BspMI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI