################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbvCI BciVI BfaI BfrI BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRV FseI FspI HaeII HindIII HpaI KasI MaeI MamI MfeI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 191 GGTACC G^GTACC AccI 940 GTCTAC GT^[AC][GT]AC Asp718I 191 GGTACC G^GTACC BbrPI 988 CACGTG CAC^GTG BclI 1503 TGATCA T^GATCA BglI 64 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 988 CACGTG [CT]AC^GT[GA] BsaI 697 GAGACC GGTCTC(1/5) BspLU11I 1691 ACATGT A^CATGT BssSI 1418 CACGAG CACGAG(-5/-1) BstEII 156 GGTGACC G^GT[ACGT]ACC BstPI 156 GGTGACC G^GT[ACGT]ACC BstXI 236 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoO65I 156 GGTGACC G^GT[ACGT]ACC EcoRI 250 GAATTC G^AATTC EcoT22I 1681 ATGCAT ATGCA^T FbaI 1503 TGATCA T^GATCA HincII 1297 GTCAAC GT[CT]^[GA]AC HindII 1297 GTCAAC GT[CT]^[GA]AC KpnI 191 GGTACC GGTAC^C KspI 1872 CCGCGG CCGC^GG MluI 776 ACGCGT A^CGCGT MslI 678 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 69 GCCGGC GCC^GGC NgoMIV 69 GCCGGC G^CCGGC NsiI 1681 ATGCAT ATGCA^T PaeR7I 1762 CTCGAG C^TCGAG PmaCI 988 CACGTG CAC^GTG PmlI 988 CACGTG CAC^GTG SacII 1872 CCGCGG CCGC^GG SfiI 63 GGCCACGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1136 GCATGC GCATG^C TfiI 1484 GAATC G^A[AT]TC XhoI 1762 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccIII 426 TCCGGA T^CCGGA 1074 TCCGGA AcsI 250 GAATTC [GA]^AATT[CT] 490 AAATTC AcyI 1441 GACGCC G[GA]^CG[CT]C 1945 GACGCC ApoI 250 GAATTC [GA]^AATT[CT] 490 AAATTC Asp700I 564 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1881 GAAACAGTTC AvaI 103 CCCGAG C^[CT]CG[GA]G 1762 CTCGAG BalI 44 TGGCCA TGG^CCA 984 TGGCCA BcgI 205 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1104 CGACAGCAGTGC BglII 170 AGATCT A^GATCT 320 AGATCT BlpI 798 GCTCAGC GC^T[ACGT]AGC 2022 GCTGAGC Bpu1102I 798 GCTCAGC GC^T[ACGT]AGC 2022 GCTGAGC BsaHI 1441 GACGCC G[GA]^CG[CT]C 1945 GACGCC BseAI 426 TCCGGA T^CCGGA 1074 TCCGGA BsmBI 286 GAGACG CGTCTC(1/5) 1831 GAGACG BsoBI 103 CCCGAG C^[CT]CG[GA]G 1762 CTCGAG BspEI 426 TCCGGA T^CCGGA 1074 TCCGGA BspHI 338 TCATGA T^CATGA 818 TCATGA BsrBI 1359 CCGCTC CCGCTC(-3/-3) 1543 GAGCGG BsrFI 69 GCCGGC [GA]^CCGG[CT] 1601 ACCGGC BssHII 76 GCGCGC G^CGCGC 78 GCGCGC CelII 798 GCTCAGC GC^T[ACGT]AGC 2022 GCTGAGC Cfr10I 69 GCCGGC [GA]^CCGG[CT] 1601 ACCGGC Hin1I 1441 GACGCC G[GA]^CG[CT]C 1945 GACGCC MluNI 44 TGGCCA TGG^CCA 984 TGGCCA MroI 426 TCCGGA T^CCGGA 1074 TCCGGA MscI 44 TGGCCA TGG^CCA 984 TGGCCA NcoI 47 CCATGG C^CATGG 1805 CCATGG PleI 328 GACTC GAGTC(4/5) 1243 GACTC PstI 1084 CTGCAG CTGCA^G 1285 CTGCAG PvuII 1636 CAGCTG CAG^CTG 1966 CAGCTG RcaI 338 TCATGA T^CATGA 818 TCATGA SacI 541 GAGCTC GAGCT^C 1510 GAGCTC SfcI 1084 CTGCAG C^T[GA][CT]AG 1285 CTGCAG SmlI 1762 CTCGAG C^T[CT][GA]AG 1864 CTCAAG Tsp45I 157 GTGAC ^GT[GC]AC 1019 GTGAC Tsp509I 251 AATT ^AATT 491 AATT XmnI 564 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1881 GAAACAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I Van91I XcmI XhoII