(Circular) (Six-base) MAPSORT of: pFN21AE1383.seq Check: 197 from: 1 to: 6772 pFN21AE1383 6772 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2413 2413 Size: 6772 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4582 278 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2946 4115 1937 Size: 1009 1169 4594 Fragments arranged by size: 4594 1169 1009 Acc65I G'GTAC_C Cuts at: 2196 4093 2196 Size: 1897 4875 AclI AA'CG_TT Cuts at: 1563 4899 5272 1563 Size: 3336 373 3063 Fragments arranged by size: 3336 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 2978 4835 6219 6487 1632 Size: 928 418 1857 1384 268 1917 Fragments arranged by size: 1917 1857 1384 928 418 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6575 AflIII A'CryG_T Cuts at: 1203 2484 2781 3696 5671 6648 1203 Size: 1281 297 915 1975 977 1327 Fragments arranged by size: 1975 1327 1281 977 915 297 AgeI A'CCGG_T Cuts at: 5603 5603 Size: 6772 AhdI GACnn_n'nnGTC Cuts at: 5501 5501 Size: 6772 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6772 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2147 3878 1184 Size: 963 1731 4078 Fragments arranged by size: 4078 1731 963 AlwNI CAG_nnn'CTG Cuts at: 2191 2879 3575 3802 3973 4515 6087 2191 Size: 688 696 227 171 542 1572 2876 Fragments arranged by size: 2876 1572 696 688 542 227 171 ApaLI G'TGCA_C Cuts at: 4829 5985 4829 Size: 1156 5616 ApoI r'AATT_y Cuts at: 1493 2255 2495 4081 4307 4459 6568 1493 Size: 762 240 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 762 240 226 152 AseI AT'TA_AT Cuts at: 160 1032 5326 160 Size: 872 4294 1606 Fragments arranged by size: 4294 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3767 4097 1870 Size: 73 165 1659 330 4545 Fragments arranged by size: 4545 1659 330 165 73 BamHI G'GATC_C Cuts at: 4102 4469 4102 Size: 367 6405 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2425 2625 3059 3985 Size: 325 217 627 409 229 200 434 926 Cuts at: 3985 4036 4093 5548 618 Size: 51 57 1455 1842 Fragments arranged by size: 1842 1455 926 627 434 409 325 229 217 200 57 51 BanII G_rGCy'C Cuts at: 1391 2115 2550 3337 3356 3519 4091 1391 Size: 724 435 787 19 163 572 4072 Fragments arranged by size: 4072 787 724 572 435 163 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2860 3379 3571 3663 928 Size: 971 961 519 192 92 4037 Fragments arranged by size: 4037 971 961 519 192 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2199 2233 3098 3132 4963 4997 1903 Size: 34 262 34 865 34 1831 34 3678 Fragments arranged by size: 3678 1831 865 262 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4664 5874 4664 Size: 1210 5562 BclI T'GATC_A Cuts at: 1548 3508 1548 Size: 1960 4812 BfrBI ATG'CAT Cuts at: 3688 3688 Size: 6772 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 5383 136 Size: 107 122 71 631 605 403 3308 1525 Fragments arranged by size: 3308 1525 631 605 403 122 107 71 BglII A'GATC_T Cuts at: 2175 2325 6767 2175 Size: 150 4442 2180 Fragments arranged by size: 4442 2180 150 BlpI GC'TnA_GC Cuts at: 2804 4028 4189 2804 Size: 1224 161 5387 Fragments arranged by size: 5387 1224 161 Bme1580I G_kGCm'C Cuts at: 1790 3062 3168 3990 4833 5989 1790 Size: 1272 106 822 843 1156 2573 Fragments arranged by size: 2573 1272 1156 843 822 106 BmgBI CAC'GTC Cuts at: 1318 2035 2485 1318 Size: 717 450 5605 Fragments arranged by size: 5605 717 450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2757 3721 4503 Size: 416 53 83 399 24 1340 964 782 Cuts at: 4503 5461 442 Size: 958 1753 Fragments arranged by size: 1753 1340 964 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2165 2252 2309 2939 3299 3632 5432 Size: 797 129 87 57 630 360 333 1800 Cuts at: 5432 1239 Size: 2579 Fragments arranged by size: 2579 1800 797 630 360 333 129 87 57 Bpu10I CC'TnA_GC Cuts at: 3336 3918 3336 Size: 582 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3854 4897 5762 6060 3854 Size: 1043 865 298 4566 Fragments arranged by size: 4566 1043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2696 5435 882 Size: 617 1197 2739 2219 Fragments arranged by size: 2739 2219 1197 617 BsaAI yAC'GTr Cuts at: 493 1204 2995 4563 493 Size: 711 1791 1568 2702 Fragments arranged by size: 2702 1791 1568 711 BsaBI GATnn'nnATC Cuts at: 6766 6766 Size: 6772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3447 3951 4579 4961 Size: 53 83 186 936 1914 504 628 382 Cuts at: 4961 5596 275 Size: 635 1451 Fragments arranged by size: 1914 1451 936 635 628 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2431 3079 3306 5205 5603 5877 6024 6435 2431 Size: 648 227 1899 398 274 147 411 2768 Fragments arranged by size: 2768 1899 648 411 398 274 227 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2837 2867 3239 3269 3446 Size: 30 165 30 1434 30 372 30 177 Cuts at: 3446 3476 3809 3839 3842 3872 1178 Size: 30 333 30 3 30 4078 Fragments arranged by size: 4078 1434 372 333 177 165 30 30 30 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2279 2312 2315 3488 3830 3848 3857 Size: 1063 30 33 3 1173 342 18 9 Cuts at: 3857 1186 Size: 4101 Fragments arranged by size: 4101 1173 1063 342 33 30 18 9 3 BseYI C'CCAG_C Cuts at: 2114 2191 2856 3643 3966 5975 6471 2114 Size: 77 665 787 323 2009 496 2415 Fragments arranged by size: 2415 2009 787 665 496 323 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 3947 3998 1163 Size: 573 1089 1122 51 3937 Fragments arranged by size: 3937 1122 1089 573 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2901 3214 3444 4222 4983 Size: 275 186 78 822 313 230 778 761 Cuts at: 4983 6011 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 822 778 761 313 275 230 186 78 BsiHKAI G_wGCw'C Cuts at: 2550 2925 3519 4833 4918 5989 2550 Size: 375 594 1314 85 1071 3333 Fragments arranged by size: 3333 1314 1071 594 375 85 BsmI GAATG_Cn' Cuts at: 4283 4376 4283 Size: 93 6679 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 3830 2285 Size: 1545 5227 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2550 2925 3062 3168 3337 3356 Size: 399 325 435 375 137 106 169 19 Cuts at: 3356 3519 3990 4091 4833 4918 5989 1391 Size: 163 471 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 435 399 375 325 169 163 137 106 101 85 19 BspEI T'CCGG_A Cuts at: 2431 3079 6435 2431 Size: 648 3356 2768 Fragments arranged by size: 3356 2768 648 BspHI T'CATG_A Cuts at: 2343 2823 4661 6391 2343 Size: 480 1838 1730 2724 Fragments arranged by size: 2724 1838 1730 480 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 2413 2847 4127 844 Size: 1364 205 434 1280 3489 Fragments arranged by size: 3489 1364 1280 434 205 BsrBI CCG'CTC Cuts at: 1394 1823 3366 3550 4659 1394 Size: 429 1543 184 1109 3507 Fragments arranged by size: 3507 1543 1109 429 184 BsrDI GCAATG_nn' Cuts at: 65 1248 5267 5441 65 Size: 1183 4019 174 1396 Fragments arranged by size: 4019 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 3606 5416 5603 1700 Size: 110 264 1532 1810 187 2869 Fragments arranged by size: 2869 1810 1532 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6772 BssHII G'CGCG_C Cuts at: 1925 2081 2083 1925 Size: 156 2 6614 Fragments arranged by size: 6614 156 2 BssSI C'ACGA_G Cuts at: 3423 4832 5844 3423 Size: 1409 1012 4351 Fragments arranged by size: 4351 1409 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3678 3802 3976 3985 794 Size: 2884 124 174 9 3581 Fragments arranged by size: 3581 2884 174 124 9 BstBI TT'CG_AA Cuts at: 5576 5576 Size: 6772 BstEII G'GTnAC_C Cuts at: 1184 2161 1184 Size: 977 5795 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 6772 BstYI r'GATC_y Cuts at: 1972 2175 2325 2434 3000 3819 3873 4102 4469 Size: 203 150 109 566 819 54 229 367 Cuts at: 4469 4854 4871 6323 6409 6421 6767 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 819 566 385 367 346 229 203 150 109 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 3064 3234 3810 3877 4017 Size: 552 939 48 1012 170 576 67 140 Cuts at: 4017 513 Size: 3268 Fragments arranged by size: 3268 1012 939 576 552 170 140 67 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2570 487 Size: 683 56 1344 4689 Fragments arranged by size: 4689 1344 683 56 BtsI GCAGTG_nn' Cuts at: 2131 3121 3908 3935 4063 4288 5082 5102 2131 Size: 990 787 27 128 225 794 20 3801 Fragments arranged by size: 3801 990 794 787 225 128 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6772 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6737 DraI TTT'AAA Cuts at: 4076 4076 Size: 6772 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5779 809 Size: 507 4463 1802 Fragments arranged by size: 4463 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2049 2076 Size: 54 1425 50 275 18 198 21 27 Cuts at: 2076 2898 2989 3231 3441 4219 5108 6737 8 Size: 822 91 242 210 778 889 1629 43 Fragments arranged by size: 1629 1425 889 822 778 275 242 210 198 91 54 50 43 27 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 2898 3441 4219 1537 Size: 275 264 822 543 778 4090 Fragments arranged by size: 4090 822 778 543 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2210 2375 2444 2451 3152 4702 1353 Size: 701 156 165 69 7 701 1550 3423 Fragments arranged by size: 3423 1550 701 701 165 156 69 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5359 5733 5879 387 Size: 1064 138 435 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2548 3517 2548 Size: 969 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2165 2252 2309 2560 2939 2978 Size: 393 404 129 87 57 251 379 39 Cuts at: 2978 3299 3632 4835 5432 6219 6487 1239 Size: 321 333 1203 597 787 268 1524 Fragments arranged by size: 1524 1203 787 597 404 393 379 333 321 268 251 129 87 57 39 EcoNI CCTnn'n_nnAGG Cuts at: 2234 3092 3278 3671 2234 Size: 858 186 393 5335 Fragments arranged by size: 5335 858 393 186 EcoO109I rG'GnC_Cy Cuts at: 2244 2836 2953 3085 3790 2244 Size: 592 117 132 705 5226 Fragments arranged by size: 5226 705 592 132 117 EcoRI G'AATT_C Cuts at: 2255 4081 4459 2255 Size: 1826 378 4568 Fragments arranged by size: 4568 1826 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 2824 Size: 6772 FspI TGC'GCA Cuts at: 5278 5278 Size: 6772 HaeII r_GCGC'y Cuts at: 1678 5919 1678 Size: 4241 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3113 3145 3239 3271 3446 Size: 32 135 32 1541 32 94 32 175 Cuts at: 3446 3478 3809 3841 5413 5445 5487 5519 1373 Size: 32 331 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 1541 331 175 135 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3304 4116 4362 669 Size: 988 281 1366 812 246 3079 Fragments arranged by size: 3079 1366 988 812 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6772 HpaI GTT'AAC Cuts at: 4362 4362 Size: 6772 KpnI G_GTAC'C Cuts at: 2200 4097 2200 Size: 1897 4875 MfeI C'AATT_G Cuts at: 4371 4371 Size: 6772 MluI A'CGCG_T Cuts at: 2781 6648 2781 Size: 3867 2905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5886 6070 1305 Size: 4581 184 2007 Fragments arranged by size: 4581 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2051 2991 10 Size: 54 1425 343 219 940 3791 Fragments arranged by size: 3791 1425 940 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2687 4730 5089 5248 518 Size: 648 1521 2043 359 159 2042 Fragments arranged by size: 2043 2042 1521 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3412 3643 3736 3879 3973 3982 4042 4188 Size: 1806 231 93 143 94 9 60 146 Cuts at: 4188 4865 6013 6258 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1806 1148 677 245 231 146 143 94 93 60 9 NaeI GCC'GGC Cuts at: 2076 2076 Size: 6772 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 3810 513 Size: 552 939 48 1758 3475 Fragments arranged by size: 3475 1758 939 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 6772 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 6772 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6772 NotI GC'GGCC_GC Cuts at: 4219 4219 Size: 6772 NsiI A_TGCA'T Cuts at: 3690 3690 Size: 6772 NspI r_CATG'y Cuts at: 1132 1229 2399 2776 2887 2929 3145 3700 4130 Size: 97 1170 377 111 42 216 555 430 Cuts at: 4130 5675 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1170 555 430 377 216 111 97 42 PasI CC'CwG_GG Cuts at: 1348 3667 1348 Size: 2319 4453 PciI A'CATG_T Cuts at: 3696 5671 3696 Size: 1975 4797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2191 3829 3973 1108 Size: 1083 1638 144 3907 Fragments arranged by size: 3907 1638 1083 144 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 6772 PmeI GTTT'AAAC Cuts at: 4076 4076 Size: 6772 PmlI CAC'GTG Cuts at: 2995 2995 Size: 6772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4841 4873 6354 6386 4841 Size: 32 1481 32 5227 Fragments arranged by size: 5227 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2836 2953 3085 2836 Size: 117 132 6523 Fragments arranged by size: 6523 132 117 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6772 PsiI TTA'TAA Cuts at: 4342 4342 Size: 6772 PspXI vC'TCGA_Gb Cuts at: 3767 3767 Size: 6772 PstI C_TGCA'G Cuts at: 830 3093 3294 4124 830 Size: 2263 201 830 3478 Fragments arranged by size: 3478 2263 830 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6772 PvuII CAG'CTG Cuts at: 3643 3973 3643 Size: 330 6442 SacI G_AGCT'C Cuts at: 2550 3519 2550 Size: 969 5803 SacII CC_GC'GG Cuts at: 3880 3880 Size: 6772 SalI G'TCGA_C Cuts at: 1936 4114 1936 Size: 2178 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2210 3152 2054 Size: 156 942 5674 Fragments arranged by size: 5674 942 156 SbfI CC_TGCA'GG Cuts at: 4124 4124 Size: 6772 ScaI AGT'ACT Cuts at: 5020 6732 5020 Size: 1712 5060 SfcI C'TryA_G Cuts at: 826 1045 3089 3290 4120 5255 5936 6127 826 Size: 219 2044 201 830 1135 681 191 1471 Fragments arranged by size: 2044 1471 1135 830 681 219 201 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2075 2075 Size: 6772 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6772 SmaI CCC'GGG Cuts at: 1872 4099 1872 Size: 2227 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3767 3869 4876 5777 6039 820 Size: 197 926 1824 102 1007 901 262 1553 Fragments arranged by size: 1824 1553 1007 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 4563 493 Size: 4070 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6772 SphI G_CATG'C Cuts at: 1132 3145 4130 1132 Size: 2013 985 3774 Fragments arranged by size: 3774 2013 985 SspI AAT'ATT Cuts at: 5 52 4696 5 Size: 47 4644 2081 Fragments arranged by size: 4644 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6772 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2247 2401 3810 4211 513 Size: 552 939 48 195 154 1409 401 3074 Fragments arranged by size: 3074 1409 939 552 401 195 154 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2613 3077 4799 4816 4969 5154 5599 Size: 1255 1033 464 1722 17 153 185 445 Cuts at: 5599 325 Size: 1498 Fragments arranged by size: 1722 1498 1255 1033 464 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5018 6730 96 Size: 274 80 33 51 1443 3041 1712 138 Fragments arranged by size: 3041 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3842 3874 6732 6764 3842 Size: 32 2858 32 3850 Fragments arranged by size: 3850 2858 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6772 XbaI T'CTAG_A Cuts at: 4108 4108 Size: 6772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2248 2665 2974 2011 Size: 237 417 309 5809 Fragments arranged by size: 5809 417 309 237 XhoI C'TCGA_G Cuts at: 1943 3767 1943 Size: 1824 4948 XmaI C'CCGG_G Cuts at: 1870 4097 1870 Size: 2227 4545 XmnI GAAnn'nnTTC Cuts at: 2573 3890 4901 2573 Size: 1317 1011 4444 Fragments arranged by size: 4444 1317 1011 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4580 276 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PsrI RsrII SanDI SexAI SfoI SrfI SwaI