(Linear) (Six-base) MAPSORT of: KIEE1383.seq Check: 9450 from: 1 to: 2067 KIEE1383.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 408 2067 Size: 408 1659 AccI GT'mk_AC Cuts at: 0 941 2067 Size: 941 1126 Acc65I G'GTAC_C Cuts at: 0 191 2067 Size: 191 1876 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 555 973 2067 Size: 555 418 1094 Fragments arranged by size: 1094 555 418 AflIII A'CryG_T Cuts at: 0 479 776 1691 2067 Size: 479 297 915 376 Fragments arranged by size: 915 479 376 297 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 142 1873 2067 Size: 142 1731 194 Fragments arranged by size: 1731 194 142 AlwNI CAG_nnn'CTG Cuts at: 0 186 874 1570 1797 1968 2067 Size: 186 688 696 227 171 99 Fragments arranged by size: 696 688 227 186 171 99 ApoI r'AATT_y Cuts at: 0 250 490 2067 Size: 250 240 1577 Fragments arranged by size: 1577 250 240 AvaI C'yCGr_G Cuts at: 0 103 1762 2067 Size: 103 1659 305 Fragments arranged by size: 1659 305 103 BanI G'GyrC_C Cuts at: 0 191 420 620 1054 1980 2031 2067 Size: 191 229 200 434 926 51 36 Fragments arranged by size: 926 434 229 200 191 51 36 BanII G_rGCy'C Cuts at: 0 110 545 1332 1351 1514 2067 Size: 110 435 787 19 163 553 Fragments arranged by size: 787 553 435 163 110 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 855 1374 1566 1658 2067 Size: 855 519 192 92 409 Fragments arranged by size: 855 519 409 192 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 194 228 1093 1127 2067 Size: 194 34 865 34 940 Fragments arranged by size: 940 865 194 34 34 BclI T'GATC_A Cuts at: 0 1503 2067 Size: 1503 564 BfrBI ATG'CAT Cuts at: 0 1683 2067 Size: 1683 384 BglI GCCn_nnn'nGGC Cuts at: 0 70 2067 Size: 70 1997 BglII A'GATC_T Cuts at: 0 170 320 2067 Size: 170 150 1747 Fragments arranged by size: 1747 170 150 BlpI GC'TnA_GC Cuts at: 0 799 2023 2067 Size: 799 1224 44 Fragments arranged by size: 1224 799 44 Bme1580I G_kGCm'C Cuts at: 0 1057 1163 1985 2067 Size: 1057 106 822 82 Fragments arranged by size: 1057 822 106 82 BmgBI CAC'GTC Cuts at: 0 30 480 2067 Size: 30 450 1587 Fragments arranged by size: 1587 450 30 BmrI ACTGGGnnnn_n' Cuts at: 0 752 1716 2067 Size: 752 964 351 Fragments arranged by size: 964 752 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 160 247 304 934 1294 1627 2067 Size: 31 129 87 57 630 360 333 440 Fragments arranged by size: 630 440 360 333 129 87 57 31 Bpu10I CC'TnA_GC Cuts at: 0 1331 1913 2067 Size: 1331 582 154 Fragments arranged by size: 1331 582 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1849 2067 Size: 1849 218 BsaI GGTCTCn'nnnn_ Cuts at: 0 691 2067 Size: 691 1376 BsaAI yAC'GTr Cuts at: 0 990 2067 Size: 990 1077 BsaHI Gr'CG_yC Cuts at: 0 1442 1946 2067 Size: 1442 504 121 Fragments arranged by size: 1442 504 121 BsaWI w'CCGG_w Cuts at: 0 426 1074 1301 2067 Size: 426 648 227 766 Fragments arranged by size: 766 648 426 227 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 832 862 1234 1264 1441 1471 1804 1834 Size: 832 30 372 30 177 30 333 30 Cuts at: 1834 1837 1867 2067 Size: 3 30 200 Fragments arranged by size: 832 372 333 200 177 30 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 244 274 307 310 1483 1825 1843 1852 Size: 244 30 33 3 1173 342 18 9 Cuts at: 1852 2067 Size: 215 Fragments arranged by size: 1173 342 244 215 33 30 18 9 3 BseYI C'CCAG_C Cuts at: 0 109 186 851 1638 1961 2067 Size: 109 77 665 787 323 106 Fragments arranged by size: 787 665 323 109 106 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 1942 1993 2067 Size: 820 1122 51 74 Fragments arranged by size: 1122 820 74 51 BsiEI CG_ry'CG Cuts at: 0 74 896 1209 1439 2067 Size: 74 822 313 230 628 Fragments arranged by size: 822 628 313 230 74 BsiHKAI G_wGCw'C Cuts at: 0 545 920 1514 2067 Size: 545 375 594 553 Fragments arranged by size: 594 553 545 375 BsmBI CGTCTCn'nnnn_ Cuts at: 0 280 1825 2067 Size: 280 1545 242 Fragments arranged by size: 1545 280 242 Bsp1286I G_dGCh'C Cuts at: 0 110 545 920 1057 1163 1332 1351 1514 Size: 110 435 375 137 106 169 19 163 Cuts at: 1514 1985 2067 Size: 471 82 Fragments arranged by size: 471 435 375 169 163 137 110 106 82 19 BspEI T'CCGG_A Cuts at: 0 426 1074 2067 Size: 426 648 993 Fragments arranged by size: 993 648 426 BspHI T'CATG_A Cuts at: 0 338 818 2067 Size: 338 480 1249 Fragments arranged by size: 1249 480 338 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 203 408 842 2067 Size: 203 205 434 1225 Fragments arranged by size: 1225 434 205 203 BsrBI CCG'CTC Cuts at: 0 1361 1545 2067 Size: 1361 184 522 Fragments arranged by size: 1361 522 184 BsrFI r'CCGG_y Cuts at: 0 69 1601 2067 Size: 69 1532 466 Fragments arranged by size: 1532 466 69 BssHII G'CGCG_C Cuts at: 0 76 78 2067 Size: 76 2 1989 Fragments arranged by size: 1989 76 2 BssSI C'ACGA_G Cuts at: 0 1418 2067 Size: 1418 649 BstAPI GCAn_nnn'nTGC Cuts at: 0 1673 1797 1971 1980 2067 Size: 1673 124 174 9 87 Fragments arranged by size: 1673 174 124 87 9 BstEII G'GTnAC_C Cuts at: 0 156 2067 Size: 156 1911 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 2067 Size: 243 1824 BstYI r'GATC_y Cuts at: 0 170 320 429 995 1814 1868 2067 Size: 170 150 109 566 819 54 199 Fragments arranged by size: 819 566 199 170 150 109 54 BtgI C'CryG_G Cuts at: 0 47 1059 1229 1805 1872 2012 2067 Size: 47 1012 170 576 67 140 55 Fragments arranged by size: 1012 576 170 140 67 55 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 565 2067 Size: 565 1502 BtsI GCAGTG_nn' Cuts at: 0 126 1116 1903 1930 2058 2067 Size: 126 990 787 27 128 9 Fragments arranged by size: 990 787 128 126 27 9 EaeI y'GGCC_r Cuts at: 0 23 44 71 893 984 1226 1436 2067 Size: 23 21 27 822 91 242 210 631 Fragments arranged by size: 822 631 242 210 91 27 23 21 EagI C'GGCC_G Cuts at: 0 71 893 1436 2067 Size: 71 822 543 631 Fragments arranged by size: 822 631 543 71 EarI CTCTTCn'nnn_ Cuts at: 0 49 205 370 439 446 1147 2067 Size: 49 156 165 69 7 701 920 Fragments arranged by size: 920 701 165 156 69 49 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 2067 Size: 19 2048 EcoICRI GAG'CTC Cuts at: 0 543 1512 2067 Size: 543 969 555 Fragments arranged by size: 969 555 543 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 160 247 304 555 934 973 1294 Size: 31 129 87 57 251 379 39 321 Cuts at: 1294 1627 2067 Size: 333 440 Fragments arranged by size: 440 379 333 321 251 129 87 57 39 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 229 1087 1273 1666 2067 Size: 229 858 186 393 401 Fragments arranged by size: 858 401 393 229 186 EcoO109I rG'GnC_Cy Cuts at: 0 239 831 948 1080 1785 2067 Size: 239 592 117 132 705 282 Fragments arranged by size: 705 592 282 239 132 117 EcoRI G'AATT_C Cuts at: 0 250 2067 Size: 250 1817 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 819 2067 Size: 819 1248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1234 1266 1441 1473 1804 1836 Size: 1108 32 94 32 175 32 331 32 Cuts at: 1836 2067 Size: 231 Fragments arranged by size: 1108 331 231 175 94 32 32 32 32 HincII GTy'rAC Cuts at: 0 1299 2067 Size: 1299 768 KpnI G_GTAC'C Cuts at: 0 195 2067 Size: 195 1872 MluI A'CGCG_T Cuts at: 0 776 2067 Size: 776 1291 MscI TGG'CCA Cuts at: 0 46 986 2067 Size: 46 940 1081 Fragments arranged by size: 1081 940 46 MslI CAynn'nnrTG Cuts at: 0 682 2067 Size: 682 1385 MspA1I CmG'CkG Cuts at: 0 1407 1638 1731 1874 1968 1977 2037 2067 Size: 1407 231 93 143 94 9 60 30 Fragments arranged by size: 1407 231 143 94 93 60 30 9 NaeI GCC'GGC Cuts at: 0 71 2067 Size: 71 1996 NcoI C'CATG_G Cuts at: 0 47 1805 2067 Size: 47 1758 262 Fragments arranged by size: 1758 262 47 NgoMIV G'CCGG_C Cuts at: 0 69 2067 Size: 69 1998 NsiI A_TGCA'T Cuts at: 0 1685 2067 Size: 1685 382 NspI r_CATG'y Cuts at: 0 394 771 882 924 1140 1695 2067 Size: 394 377 111 42 216 555 372 Fragments arranged by size: 555 394 377 372 216 111 42 PasI CC'CwG_GG Cuts at: 0 1662 2067 Size: 1662 405 PciI A'CATG_T Cuts at: 0 1691 2067 Size: 1691 376 PflMI CCAn_nnn'nTGG Cuts at: 0 186 1824 1968 2067 Size: 186 1638 144 99 Fragments arranged by size: 1638 186 144 99 PfoI T'CCnGG_A Cuts at: 0 809 2067 Size: 809 1258 PmlI CAC'GTG Cuts at: 0 990 2067 Size: 990 1077 PpuMI rG'GwC_Cy Cuts at: 0 831 948 1080 2067 Size: 831 117 132 987 Fragments arranged by size: 987 831 132 117 PspXI vC'TCGA_Gb Cuts at: 0 1762 2067 Size: 1762 305 PstI C_TGCA'G Cuts at: 0 1088 1289 2067 Size: 1088 201 778 Fragments arranged by size: 1088 778 201 PvuII CAG'CTG Cuts at: 0 1638 1968 2067 Size: 1638 330 99 Fragments arranged by size: 1638 330 99 SacI G_AGCT'C Cuts at: 0 545 1514 2067 Size: 545 969 553 Fragments arranged by size: 969 553 545 SacII CC_GC'GG Cuts at: 0 1875 2067 Size: 1875 192 SapI GCTCTTCn'nnn_ Cuts at: 0 49 205 1147 2067 Size: 49 156 942 920 Fragments arranged by size: 942 920 156 49 SfcI C'TryA_G Cuts at: 0 1084 1285 2067 Size: 1084 201 782 Fragments arranged by size: 1084 782 201 SfiI GGCCn_nnn'nGGCC Cuts at: 0 70 2067 Size: 70 1997 SmlI C'TyrA_G Cuts at: 0 1762 1864 2067 Size: 1762 102 203 Fragments arranged by size: 1762 203 102 SphI G_CATG'C Cuts at: 0 1140 2067 Size: 1140 927 StyI C'CwwG_G Cuts at: 0 47 242 396 1805 2067 Size: 47 195 154 1409 262 Fragments arranged by size: 1409 262 195 154 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 608 1072 2067 Size: 608 464 995 Fragments arranged by size: 995 608 464 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1837 1869 2067 Size: 1837 32 198 Fragments arranged by size: 1837 198 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 243 660 969 2067 Size: 243 417 309 1098 Fragments arranged by size: 1098 417 309 243 XhoI C'TCGA_G Cuts at: 0 1762 2067 Size: 1762 305 XmnI GAAnn'nnTTC Cuts at: 0 568 1885 2067 Size: 568 1317 182 Fragments arranged by size: 1317 568 182 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApoI AvaI BanI BanII BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII KpnI MluI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacI SacII SapI SfcI SfiI SmlI SphI StyI TaqII TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BmtI BplI BsaBI BsiWI BsmI BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MmeI NarI NdeI NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XmaI ZraI