Restriction map for
pFN21AE6139
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# NO CUT ENZYMES ( 176 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 262 GACGTC GACGT^C
AccII 18 CGCG CG^CG
AluI 257 AGCT AG^CT
AlwI 117 GATCC GGATC(4/5)
ApaI 39 GGGCCC GGGCC^C
BanI 7 GGCGCC G^G[CT][GA]CC
BbeI 7 GGCGCC GGCGC^C
BbsI 169 GTCTTC GAAGAC(2/6)
BpuAI 169 GTCTTC GAAGAC(2/6)
BsmFI 49 GTCCC GGGAC(10/14)
BsrI 36 ACTGG ACTGG(1/-1)
BssSI 199 CTCGTG CACGAG(-5/-1)
BstUI 18 CGCG CG^CG
Cac8I 212 GCGTGC GC[ACGT]^[ACGT]GC
DpnII 117 GATC ^GATC
DraII 39 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 174 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 39 GGGCCCC [GA]G^G[ACGT]CC[CT]
HgaI 31 GACGC GACGC(5/10)
HinfI 250 GACTC G^A[ACGT]TC
KasI 7 GGCGCC G^GCGCC
MboI 117 GATC ^GATC
MspA1I 67 CCGCTG C[AC]G^C[GT]G
MvnI 18 CGCG CG^CG
NarI 7 GGCGCC GG^CGCC
NdeII 117 GATC ^GATC
PleI 250 GACTC GAGTC(4/5)
SacI 256 GAGCTC GAGCT^C
Sau3AI 117 GATC ^GATC
SfoI 7 GGCGCC GGC^GCC
SmaI 11 CCCGGG CCC^GGG
TaqI 260 TCGA T^CGA
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 260 TCGA T^CGA
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 7 GGCGCC G[GA]^CG[CT]C
262 GACGTC
AspHI 214 GTGCTC G[AT]GC[AT]^C
256 GAGCTC
BanII 39 GGGCCC G[GA]GC[CT]^C
256 GAGCTC
BbvI 69 GCTGC GCAGC(8/12)
72 GCTGC
BsaAI 55 TACGTG [CT]AC^GT[GA]
88 TACGTG
BsaHI 7 GGCGCC G[GA]^CG[CT]C
262 GACGTC
BsiHKAI 214 GTGCTC G[AT]GC[AT]^C
256 GAGCTC
CfoI 8 GCGC GCG^C
196 GCGC
HaeII 7 GGCGCC [GA]GCGC^[CT]
195 GGCGCT
HapII 12 CCGG C^CGG
227 CCGG
HhaI 8 GCGC GCG^C
196 GCGC
Hin1I 7 GGCGCC G[GA]^CG[CT]C
262 GACGTC
HinP1I 8 GCGC G^CGC
196 GCGC
HpaII 12 CCGG C^CGG
227 CCGG
MboII 101 TCTTC GAAGA(8/7)
170 TCTTC
MspI 12 CCGG C^CGG
227 CCGG
NlaIII 138 CATG CATG^
186 CATG
SfaNI 244 GATGC GCATC(5/9)
271 GATGC
TseI 69 GCTGC G^C[AT]GC
72 GCTGC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AvaI AvaII BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstNI Cfr13I EcoRII
Fnu4HI HaeIII ItaI MaeII MnlI
MvaI MwoI NciI NlaIV RsaI
Sau96I ScrFI