(Circular) (Six-base) MAPSORT of: pFN21AE6139.seq Check: 1775 from: 1 to: 4990 pFN21AE6139 4990 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 2800 278 Size: 53 83 186 936 735 529 2468 Fragments arranged by size: 2468 936 735 529 186 83 53 AccI GT'mk_AC Cuts at: 1937 2333 1937 Size: 396 4594 Acc65I G'GTAC_C Cuts at: 2311 2311 Size: 4990 AclI AA'CG_TT Cuts at: 1563 3117 3490 1563 Size: 1554 373 3063 Fragments arranged by size: 3063 1554 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3053 4437 4705 1632 Size: 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4793 AflIII A'CryG_T Cuts at: 1203 3889 4866 1203 Size: 2686 977 1327 Fragments arranged by size: 2686 1327 977 AgeI A'CCGG_T Cuts at: 3821 3821 Size: 4990 AhdI GACnn_n'nnGTC Cuts at: 3719 3719 Size: 4990 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4990 AlwNI CAG_nnn'CTG Cuts at: 2733 4305 2733 Size: 1572 3418 ApaI G_GGCC'C Cuts at: 2048 2048 Size: 4990 ApaLI G'TGCA_C Cuts at: 3047 4203 3047 Size: 1156 3834 ApoI r'AATT_y Cuts at: 1493 2299 2525 2677 4786 1493 Size: 806 226 152 2109 1697 Fragments arranged by size: 2109 1697 806 226 152 AseI AT'TA_AT Cuts at: 160 1032 3544 160 Size: 872 2512 1606 Fragments arranged by size: 2512 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4990 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2183 2236 2315 1870 Size: 73 73 167 53 79 4545 Fragments arranged by size: 4545 167 79 73 73 53 BamHI G'GATC_C Cuts at: 2320 2687 2320 Size: 367 4623 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2311 3766 618 Size: 325 217 627 225 299 1455 1842 Fragments arranged by size: 1842 1455 627 325 299 225 217 BanII G_rGCy'C Cuts at: 1391 2048 2265 2309 1391 Size: 657 217 44 4072 Fragments arranged by size: 4072 657 217 44 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 4990 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 928 Size: 971 268 3751 Fragments arranged by size: 3751 971 268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3181 3215 1903 Size: 34 50 34 1160 34 3678 Fragments arranged by size: 3678 1160 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2882 4092 2882 Size: 1210 3780 BclI T'GATC_A Cuts at: 1548 1548 Size: 4990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3601 136 Size: 107 122 71 631 605 1929 1525 Fragments arranged by size: 1929 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4985 4985 Size: 4990 BlpI GC'TnA_GC Cuts at: 2407 2407 Size: 4990 Bme1580I G_kGCm'C Cuts at: 1790 2048 3051 4207 1790 Size: 258 1003 1156 2573 Fragments arranged by size: 2573 1156 1003 258 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4990 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2051 2721 3679 Size: 416 53 83 399 24 634 670 958 Cuts at: 3679 442 Size: 1753 Fragments arranged by size: 1753 958 670 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4990 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3650 1239 Size: 2411 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3115 3980 4278 3115 Size: 865 298 3827 Fragments arranged by size: 3827 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3653 882 Size: 617 2154 2219 Fragments arranged by size: 2219 2154 617 BsaAI yAC'GTr Cuts at: 493 1204 2062 2095 2781 493 Size: 711 858 33 686 2702 Fragments arranged by size: 2702 858 711 686 33 BsaBI GATnn'nnATC Cuts at: 4984 4984 Size: 4990 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2268 2797 3179 Size: 53 83 186 936 480 255 529 382 Cuts at: 3179 3814 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 529 480 382 255 186 83 53 BsaWI w'CCGG_w Cuts at: 3423 3821 4095 4242 4653 3423 Size: 398 274 147 411 3760 Fragments arranged by size: 3760 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4765 Fragments arranged by size: 4765 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4990 BseYI C'CCAG_C Cuts at: 4193 4689 4193 Size: 496 4494 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2440 3201 4229 1540 Size: 275 186 439 761 1028 2301 Fragments arranged by size: 2301 1028 761 439 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2265 3051 3136 4207 2223 Size: 42 786 85 1071 3006 Fragments arranged by size: 3006 1071 786 85 42 BsmI GAATG_Cn' Cuts at: 2501 2594 2501 Size: 93 4897 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2223 2265 2309 3051 3136 4207 Size: 399 258 175 42 44 742 85 1071 Cuts at: 4207 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 258 175 85 44 42 BspEI T'CCGG_A Cuts at: 4653 4653 Size: 4990 BspHI T'CATG_A Cuts at: 2879 4609 2879 Size: 1730 3260 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 844 Size: 1501 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2877 1394 Size: 429 1054 3507 Fragments arranged by size: 3507 1054 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3485 3659 65 Size: 1183 2237 174 1396 Fragments arranged by size: 2237 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3634 3821 1700 Size: 110 1824 187 2869 Fragments arranged by size: 2869 1824 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4990 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4990 BssSI C'ACGA_G Cuts at: 2204 3050 4062 2204 Size: 846 1012 3132 Fragments arranged by size: 3132 1012 846 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4990 BstBI TT'CG_AA Cuts at: 3794 3794 Size: 4990 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4990 BstYI r'GATC_y Cuts at: 1972 2320 2687 3072 3089 4541 4627 4639 4985 Size: 348 367 385 17 1452 86 12 346 Cuts at: 4985 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 348 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4438 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4251 Fragments arranged by size: 4251 683 56 BtsI GCAGTG_nn' Cuts at: 2506 3300 3320 2506 Size: 794 20 4176 Fragments arranged by size: 4176 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4990 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4955 DraI TTT'AAA Cuts at: 2294 2294 Size: 4990 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3997 809 Size: 507 2681 1802 Fragments arranged by size: 2681 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 3326 4955 Size: 54 1425 50 275 18 607 889 1629 Cuts at: 4955 8 Size: 43 Fragments arranged by size: 1629 1425 889 607 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2437 1537 Size: 275 625 4090 Fragments arranged by size: 4090 625 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2920 1353 Size: 1567 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3577 3951 4097 387 Size: 1064 138 1988 374 146 1280 Fragments arranged by size: 1988 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2263 2263 Size: 4990 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3053 3650 4437 4705 1239 Size: 393 1421 597 787 268 1524 Fragments arranged by size: 1524 1421 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2183 2183 Size: 4990 EcoO109I rG'GnC_Cy Cuts at: 2045 2045 Size: 4990 EcoRI G'AATT_C Cuts at: 2299 2677 2299 Size: 378 4612 FspI TGC'GCA Cuts at: 3496 3496 Size: 4990 HaeII r_GCGC'y Cuts at: 1678 2016 2204 4137 1678 Size: 338 188 1933 2531 Fragments arranged by size: 2531 1933 338 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2240 2251 2272 3631 Size: 32 135 32 647 21 11 21 1359 Cuts at: 3631 3663 3705 3737 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 647 135 42 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2334 2580 669 Size: 988 281 396 246 3079 Fragments arranged by size: 3079 988 396 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4990 HpaI GTT'AAC Cuts at: 2580 2580 Size: 4990 KasI G'GCGC_C Cuts at: 2012 2012 Size: 4990 KpnI G_GTAC'C Cuts at: 2315 2315 Size: 4990 MfeI C'AATT_G Cuts at: 2589 2589 Size: 4990 MluI A'CGCG_T Cuts at: 4866 4866 Size: 4990 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 4104 4288 1305 Size: 945 1854 184 2007 Fragments arranged by size: 2007 1854 945 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3168 Fragments arranged by size: 3168 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2948 3307 3466 518 Size: 648 1782 359 159 2042 Fragments arranged by size: 2042 1782 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2406 3083 4231 4476 1606 Size: 468 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 468 332 245 NarI GG'CG_CC Cuts at: 2013 2013 Size: 4990 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4438 NdeI CA'TA_TG Cuts at: 387 387 Size: 4990 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4990 NotI GC'GGCC_GC Cuts at: 2437 2437 Size: 4990 NspI r_CATG'y Cuts at: 1132 1229 2348 3893 1132 Size: 97 1119 1545 2229 Fragments arranged by size: 2229 1545 1119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4990 PciI A'CATG_T Cuts at: 3889 3889 Size: 4990 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4990 PmeI GTTT'AAAC Cuts at: 2294 2294 Size: 4990 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3059 3091 4572 4604 3059 Size: 32 1481 32 3445 Fragments arranged by size: 3445 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4990 PsiI TTA'TAA Cuts at: 2560 2560 Size: 4990 PspOMI G'GGCC_C Cuts at: 2044 2044 Size: 4990 PstI C_TGCA'G Cuts at: 830 2342 830 Size: 1512 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4990 SacI G_AGCT'C Cuts at: 2265 2265 Size: 4990 SalI G'TCGA_C Cuts at: 1936 2332 1936 Size: 396 4594 SbfI CC_TGCA'GG Cuts at: 2342 2342 Size: 4990 ScaI AGT'ACT Cuts at: 3238 4950 3238 Size: 1712 3278 SfcI C'TryA_G Cuts at: 826 1045 2338 3473 4154 4345 826 Size: 219 1293 1135 681 191 1471 Fragments arranged by size: 1471 1293 1135 681 219 191 SfoI GGC'GCC Cuts at: 2014 2014 Size: 4990 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4990 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4990 SmaI CCC'GGG Cuts at: 1872 2018 2317 1872 Size: 146 299 4545 Fragments arranged by size: 4545 299 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 3094 3995 4257 820 Size: 197 926 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2781 493 Size: 2288 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4990 SphI G_CATG'C Cuts at: 1132 2348 1132 Size: 1216 3774 SspI AAT'ATT Cuts at: 5 52 2914 5 Size: 47 2862 2081 Fragments arranged by size: 2862 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4990 StyI C'CwwG_G Cuts at: 513 1065 2429 513 Size: 552 1364 3074 Fragments arranged by size: 3074 1364 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3034 3187 3372 3817 325 Size: 1255 1437 17 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 2148 3236 Size: 274 80 33 51 1443 150 21 1088 Cuts at: 3236 4948 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1088 274 150 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4950 4982 4950 Size: 32 4958 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4990 XbaI T'CTAG_A Cuts at: 2326 2326 Size: 4990 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4990 XmaI C'CCGG_G Cuts at: 1870 2016 2315 1870 Size: 146 299 4545 Fragments arranged by size: 4545 299 146 XmnI GAAnn'nnTTC Cuts at: 3119 3119 Size: 4990 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 2798 276 Size: 53 83 186 936 735 529 2468 Fragments arranged by size: 2468 936 735 529 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI