################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AvaII AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BpmI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I Cfr10I ClaI CpoI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SphI Sse8387I SspBI StuI SwaI TfiI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AfaI 686 GTAC GT^AC AseI 657 ATTAAT AT^TAAT AsnI 657 ATTAAT AT^TAAT Asp700I 421 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 209 GAGCTC G[AT]GC[AT]^C AvrII 904 CCTAGG C^CTAGG BbsI 888 GAAGAC GAAGAC(2/6) BbvI 41 GCTGC GCAGC(8/12) BclI 554 TGATCA T^GATCA BlnI 904 CCTAGG C^CTAGG BlpI 52 GCTCAGC GC^T[ACGT]AGC Bpu1102I 52 GCTCAGC GC^T[ACGT]AGC BpuAI 888 GAAGAC GAAGAC(2/6) BsaAI 163 TACGTA [CT]AC^GT[GA] BsaWI 505 TCCGGT [AT]^CCGG[AT] BsiHKAI 209 GAGCTC G[AT]GC[AT]^C BsmI 615 GAATGC GAATGC(1/-1) BsrDI 714 CATTGC GCAATG(2/0) BsrI 622 ACTGG ACTGG(1/-1) BstF5I 516 GGATG GGATG(2/0) BstYI 502 AGATCC [GA]^GATC[CT] Cac8I 37 GCGTGC GC[ACGT]^[ACGT]GC CelII 52 GCTCAGC GC^T[ACGT]AGC CfoI 19 GCGC GCG^C DraI 178 TTTAAA TTT^AAA DrdI 103 GACACTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 85 GAATTC G^AATTC EcoT22I 796 ATGCAT ATGCA^T FbaI 554 TGATCA T^GATCA FokI 516 GGATG GGATG(9/13) HaeII 18 GGCGCT [GA]GCGC^[CT] HapII 506 CCGG C^CGG HhaI 19 GCGC GCG^C HinP1I 19 GCGC G^CGC HinfI 768 GAGTC G^A[ACGT]TC HpaII 506 CCGG C^CGG MfeI 527 CAATTG C^AATTG MflI 502 AGATCC [GA]^GATC[CT] MslI 670 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 506 CCGG C^CGG MunI 527 CAATTG C^AATTG MwoI 32 GCTCGGCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 796 ATGCAT ATGCA^T PflMI 793 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 768 GAGTC GAGTC(4/5) PshBI 657 ATTAAT AT^TAAT RsaI 686 GTAC GT^AC SacI 209 GAGCTC GAGCT^C SnaBI 163 TACGTA TAC^GTA TaqI 380 TCGA T^CGA TseI 41 GCTGC G^C[AT]GC Tsp45I 640 GTGAC ^GT[GC]AC TthHB8I 380 TCGA T^CGA Van91I 793 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 502 AGATCC [GA]^GATC[CT] XmnI 421 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 160 GTATAC GT^[AC][GT]AC 788 GTATAC AlwI 503 GATCC GGATC(4/5) 46 GGATC AspI 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 764 GACTGAGTC BanII 30 GGGCTC G[GA]GC[CT]^C 209 GAGCTC BmyI 30 GGGCTC G[AGT]GC[ACT]^C 209 GAGCTC Bsp1286I 30 GGGCTC G[AGT]GC[ACT]^C 209 GAGCTC BssKI 9 CCTGG ^CC[ACGT]GG 595 CCAGG Bst1107I 160 GTATAC GTA^TAC 788 GTATAC BstNI 9 CCTGG CC^[AT]GG 595 CCAGG BstZ17I 160 GTATAC GTA^TAC 788 GTATAC Cfr13I 5 GGCCC G^G[ACGT]CC 802 GGCCC EarI 806 CTCTTC CTCTTC(1/4) 185 GAAGAG EcoRII 9 CCTGG ^CC[AT]GG 595 CCAGG EcoT14I 627 CCTTGG C^C[AT][AT]GG 904 CCTAGG Ksp632I 806 CTCTTC CTCTTC(1/4) 185 GAAGAG MaeII 91 ACGT A^CGT 164 ACGT MvaI 9 CCTGG CC^[AT]GG 595 CCAGG NlaIV 5 GGCCCC GG[ACGT]^[ACGT]CC 577 GGAGCC PflFI 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 764 GACTGAGTC Sau96I 5 GGCCC G^G[ACGT]CC 802 GGCCC ScrFI 9 CCTGG CC^[ACGT]GG 595 CCAGG SfaNI 283 GATGC GCATC(5/9) 310 GATGC SfcI 80 CTGTAG C^T[GA][CT]AG 647 CTACAG SpeI 549 ACTAGT A^CTAGT 855 ACTAGT StyI 627 CCTTGG C^C[AT][AT]GG 904 CCTAGG Tth111I 103 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 764 GACTGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AcsI AluI ApoI BfaI BsaJI BsiYI BslI BsmAI BsmFI DdeI DpnII Fnu4HI HaeIII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII Sau3AI SspI Tru9I Tsp509I TspRI