(Circular) (Six-base) MAPSORT of: pFN21AE6713.seq Check: 297 from: 1 to: 6868 pFN21AE6713 6868 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2340 4678 278 Size: 53 83 186 936 804 2338 2468 Fragments arranged by size: 2468 2338 936 804 186 83 53 AccI GT'mk_AC Cuts at: 1937 4211 1937 Size: 2274 4594 Acc65I G'GTAC_C Cuts at: 2709 3759 4189 2709 Size: 1050 430 5388 Fragments arranged by size: 5388 1050 430 AclI AA'CG_TT Cuts at: 1563 4995 5368 1563 Size: 3432 373 3063 Fragments arranged by size: 3432 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3676 3739 4931 6315 6583 1632 Size: 2044 63 1192 1384 268 1917 Fragments arranged by size: 2044 1917 1384 1192 268 63 AfeI AGC'GCT Cuts at: 2984 2984 Size: 6868 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6671 AflIII A'CryG_T Cuts at: 1203 2167 5767 6744 1203 Size: 964 3600 977 1327 Fragments arranged by size: 3600 1327 977 964 AgeI A'CCGG_T Cuts at: 5699 5699 Size: 6868 AhdI GACnn_n'nnGTC Cuts at: 5597 5597 Size: 6868 AleI CACnn'nnGTG Cuts at: 1166 2348 2774 3305 1166 Size: 1182 426 531 4729 Fragments arranged by size: 4729 1182 531 426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6868 AlwNI CAG_nnn'CTG Cuts at: 2742 2935 3181 3360 3418 4611 6183 2742 Size: 193 246 179 58 1193 1572 3427 Fragments arranged by size: 3427 1572 1193 246 193 179 58 ApaI G_GGCC'C Cuts at: 2137 2650 3495 2137 Size: 513 845 5510 Fragments arranged by size: 5510 845 513 ApaLI G'TGCA_C Cuts at: 3239 4925 6081 3239 Size: 1686 1156 4026 Fragments arranged by size: 4026 1686 1156 ApoI r'AATT_y Cuts at: 1493 3065 4079 4177 4403 4555 6664 1493 Size: 1572 1014 98 226 152 2109 1697 Fragments arranged by size: 2109 1697 1572 1014 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 5422 160 Size: 872 4390 1606 Fragments arranged by size: 4390 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2025 2137 2218 2231 2917 3774 Size: 73 70 12 112 81 13 686 857 Cuts at: 3774 3977 4161 4193 1870 Size: 203 184 32 4545 Fragments arranged by size: 4545 857 686 203 184 112 81 73 70 32 13 12 BamHI G'GATC_C Cuts at: 2511 4198 4565 2511 Size: 1687 367 4814 Fragments arranged by size: 4814 1687 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2452 2709 3661 3759 Size: 325 217 627 232 433 257 952 98 Cuts at: 3759 3868 4189 5644 618 Size: 109 321 1455 1842 Fragments arranged by size: 1842 1455 952 627 433 325 321 257 232 217 109 98 BanII G_rGCy'C Cuts at: 1391 2137 2292 2643 2650 2853 3129 3495 3966 Size: 746 155 351 7 203 276 366 471 Cuts at: 3966 4157 4187 1391 Size: 191 30 4072 Fragments arranged by size: 4072 746 471 366 351 276 203 191 155 30 7 BbeI G_GCGC'C Cuts at: 2023 2023 Size: 6868 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 3547 928 Size: 971 1308 340 4249 Fragments arranged by size: 4249 1308 971 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5059 5093 1903 Size: 34 50 34 3038 34 3678 Fragments arranged by size: 3678 3038 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4760 5970 4760 Size: 1210 5658 BclI T'GATC_A Cuts at: 1548 2904 1548 Size: 1356 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2122 5479 Size: 107 122 71 631 605 345 105 3357 Cuts at: 5479 136 Size: 1525 Fragments arranged by size: 3357 1525 631 605 345 122 107 105 71 BglII A'GATC_T Cuts at: 6863 6863 Size: 6868 BlpI GC'TnA_GC Cuts at: 2580 3162 3408 4285 2580 Size: 582 246 877 5163 Fragments arranged by size: 5163 877 582 246 Bme1580I G_kGCm'C Cuts at: 1790 2137 2650 3140 3243 3495 3743 4929 6085 Size: 347 513 490 103 252 248 1186 1156 Cuts at: 6085 1790 Size: 2573 Fragments arranged by size: 2573 1186 1156 513 490 347 252 248 103 BmgBI CAC'GTC Cuts at: 1318 3463 1318 Size: 2145 4723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2559 4599 5557 Size: 416 53 83 399 24 1142 2040 958 Cuts at: 5557 442 Size: 1753 Fragments arranged by size: 2040 1753 1142 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 2447 3116 4021 4046 5528 1239 Size: 1072 136 669 905 25 1482 2579 Fragments arranged by size: 2579 1482 1072 905 669 136 25 Bpu10I CC'TnA_GC Cuts at: 2855 3816 2855 Size: 961 5907 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 2993 4055 4993 5858 6156 2477 Size: 516 1062 938 865 298 3189 Fragments arranged by size: 3189 1062 938 865 516 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2208 2509 3668 5531 882 Size: 617 709 301 1159 1863 2219 Fragments arranged by size: 2219 1863 1159 709 617 301 BsaAI yAC'GTr Cuts at: 493 1204 2168 3862 4659 493 Size: 711 964 1694 797 2702 Fragments arranged by size: 2702 1694 964 797 711 BsaBI GATnn'nnATC Cuts at: 6862 6862 Size: 6868 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2337 2406 2999 Size: 53 83 186 936 487 317 69 593 Cuts at: 2999 4675 5057 5692 275 Size: 1676 382 635 1451 Fragments arranged by size: 1676 1451 936 635 593 487 382 317 186 83 69 53 BsaWI w'CCGG_w Cuts at: 5301 5699 5973 6120 6531 5301 Size: 398 274 147 411 5638 Fragments arranged by size: 5638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 2373 2403 2810 Size: 30 165 30 828 30 112 30 407 Cuts at: 2810 2840 1178 Size: 30 5206 Fragments arranged by size: 5206 828 407 165 112 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6868 BseYI C'CCAG_C Cuts at: 2032 2814 3140 3312 3625 3631 4132 6071 6567 Size: 782 326 172 313 6 501 1939 496 Cuts at: 6567 2032 Size: 2333 Fragments arranged by size: 2333 1939 782 501 496 326 313 172 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 2948 2975 3373 1163 Size: 573 633 579 27 398 4658 Fragments arranged by size: 4658 633 579 573 398 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2682 4318 5079 6107 1540 Size: 275 186 681 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 681 275 186 BsiHKAI G_wGCw'C Cuts at: 2292 2643 2853 2916 3129 3243 3966 4929 5014 Size: 351 210 63 213 114 723 963 85 Cuts at: 5014 6085 2292 Size: 1071 3075 Fragments arranged by size: 3075 1071 963 723 351 213 210 114 85 63 BsmI GAATG_Cn' Cuts at: 4379 4472 4379 Size: 93 6775 BsmBI CGTCTCn'nnnn_ Cuts at: 2328 2423 2328 Size: 95 6773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2292 2643 2650 2853 2916 3129 Size: 399 347 155 351 7 203 63 213 Cuts at: 3129 3140 3243 3495 3743 3966 4157 4187 4929 Size: 11 103 252 248 223 191 30 742 Cuts at: 4929 5014 6085 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 347 252 248 223 213 203 191 155 103 85 63 30 11 7 BspEI T'CCGG_A Cuts at: 6531 6531 Size: 6868 BspHI T'CATG_A Cuts at: 4757 6487 4757 Size: 1730 5138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3120 4223 844 Size: 2276 1103 3489 Fragments arranged by size: 3489 2276 1103 BsrBI CCG'CTC Cuts at: 1394 1823 2129 2158 2247 4035 4755 1394 Size: 429 306 29 89 1788 720 3507 Fragments arranged by size: 3507 1788 720 429 306 89 29 BsrDI GCAATG_nn' Cuts at: 65 1248 3842 5363 5537 65 Size: 1183 2594 1521 174 1396 Fragments arranged by size: 2594 1521 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2225 2796 3327 3639 3664 5512 5699 Size: 110 415 571 531 312 25 1848 187 Cuts at: 5699 1700 Size: 2869 Fragments arranged by size: 2869 1848 571 531 415 312 187 110 25 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6868 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6868 BssSI C'ACGA_G Cuts at: 3764 3873 4928 5940 3764 Size: 109 1055 1012 4692 Fragments arranged by size: 4692 1055 1012 109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2935 3247 3418 794 Size: 2141 312 171 4244 Fragments arranged by size: 4244 2141 312 171 BstBI TT'CG_AA Cuts at: 5672 5672 Size: 6868 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6868 BstXI CCAn_nnnn'nTGG Cuts at: 2875 3379 2875 Size: 504 6364 BstYI r'GATC_y Cuts at: 1972 2511 4198 4565 4950 4967 6419 6505 6517 Size: 539 1687 367 385 17 1452 86 12 Cuts at: 6517 6863 1972 Size: 346 1977 Fragments arranged by size: 1977 1687 1452 539 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3605 3605 Size: 6868 BtgI C'CryG_G Cuts at: 513 1065 2409 3321 4105 513 Size: 552 1344 912 784 3276 Fragments arranged by size: 3276 1344 912 784 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6129 Fragments arranged by size: 6129 683 56 BtsI GCAGTG_nn' Cuts at: 2381 4384 5178 5198 2381 Size: 2003 794 20 4051 Fragments arranged by size: 4051 2003 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6868 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6833 DraI TTT'AAA Cuts at: 4172 4172 Size: 6868 DraIII CAC_nnn'GTG Cuts at: 2398 3158 3247 3937 2398 Size: 760 89 690 5329 Fragments arranged by size: 5329 760 690 89 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2612 5875 809 Size: 507 1296 3263 1802 Fragments arranged by size: 3263 1802 1296 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2412 2976 Size: 54 1425 50 275 18 471 111 564 Cuts at: 2976 3843 4315 5204 6833 8 Size: 867 472 889 1629 43 Fragments arranged by size: 1629 1425 889 867 564 472 471 275 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4315 1537 Size: 275 2503 4090 Fragments arranged by size: 4090 2503 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 2441 4798 1353 Size: 1016 72 2357 3423 Fragments arranged by size: 3423 2357 1016 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5455 5829 5975 387 Size: 1064 138 3866 374 146 1280 Fragments arranged by size: 3866 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2290 2641 2851 3127 3964 2290 Size: 351 210 276 837 5194 Fragments arranged by size: 5194 837 351 276 210 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2447 3116 3676 3739 4021 4046 Size: 393 679 136 669 560 63 282 25 Cuts at: 4046 4931 5528 6315 6583 1239 Size: 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 679 669 597 560 393 282 268 136 63 25 EcoNI CCTnn'n_nnAGG Cuts at: 4161 4161 Size: 6868 EcoO109I rG'GnC_Cy Cuts at: 2092 2133 2134 2647 2739 3178 3491 3492 3538 Size: 41 1 513 92 439 313 1 46 Cuts at: 3538 3609 3820 4066 2092 Size: 71 211 246 4894 Fragments arranged by size: 4894 513 439 313 246 211 92 71 46 41 1 1 EcoRI G'AATT_C Cuts at: 3065 4079 4177 4555 3065 Size: 1014 98 378 5378 Fragments arranged by size: 5378 1014 378 98 FseI GG_CCGG'CC Cuts at: 2229 2229 Size: 6868 FspI TGC'GCA Cuts at: 5374 5374 Size: 6868 HaeII r_GCGC'y Cuts at: 1678 2023 2033 2110 2986 6015 1678 Size: 345 10 77 876 3029 2531 Fragments arranged by size: 3029 2531 876 345 77 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2325 2357 5509 5541 5583 Size: 32 135 32 753 32 3152 32 42 Cuts at: 5583 5615 1373 Size: 32 2626 Fragments arranged by size: 3152 2626 753 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3508 4212 4458 669 Size: 988 281 1570 704 246 3079 Fragments arranged by size: 3079 1570 988 704 281 246 HindIII A'AGCT_T Cuts at: 748 3038 748 Size: 2290 4578 HpaI GTT'AAC Cuts at: 4458 4458 Size: 6868 KasI G'GCGC_C Cuts at: 2019 2019 Size: 6868 KpnI G_GTAC'C Cuts at: 2713 3763 4193 2713 Size: 1050 430 5388 Fragments arranged by size: 5388 1050 430 MfeI C'AATT_G Cuts at: 4467 4467 Size: 6868 MluI A'CGCG_T Cuts at: 6744 6744 Size: 6868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3321 5982 6166 1305 Size: 969 1047 2661 184 2007 Fragments arranged by size: 2661 2007 1047 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2414 10 Size: 54 1425 343 471 111 4464 Fragments arranged by size: 4464 1425 471 343 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2447 2774 3305 4826 5185 5344 Size: 648 1182 99 327 531 1521 359 159 Cuts at: 5344 518 Size: 2042 Fragments arranged by size: 2042 1521 1182 648 531 359 327 159 99 MspA1I CmG'CkG Cuts at: 1606 2959 3316 3625 3804 4284 4961 6109 6354 Size: 1353 357 309 179 480 677 1148 245 Cuts at: 6354 1606 Size: 2120 Fragments arranged by size: 2120 1353 1148 677 480 357 309 245 179 NaeI GCC'GGC Cuts at: 2227 3641 3666 2227 Size: 1414 25 5429 Fragments arranged by size: 5429 1414 25 NarI GG'CG_CC Cuts at: 2020 2020 Size: 6868 NcoI C'CATG_G Cuts at: 513 1065 3321 4105 513 Size: 552 2256 784 3276 Fragments arranged by size: 3276 2256 784 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6868 NgoMIV G'CCGG_C Cuts at: 2225 3639 3664 2225 Size: 1414 25 5429 Fragments arranged by size: 5429 1414 25 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6868 NotI GC'GGCC_GC Cuts at: 4315 4315 Size: 6868 NspI r_CATG'y Cuts at: 1132 1229 2300 3062 3254 4226 5771 1132 Size: 97 1071 762 192 972 1545 2229 Fragments arranged by size: 2229 1545 1071 972 762 192 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6868 PciI A'CATG_T Cuts at: 5767 5767 Size: 6868 PflMI CCAn_nnn'nTGG Cuts at: 1108 3937 1108 Size: 2829 4039 PfoI T'CCnGG_A Cuts at: 2424 2507 2601 3093 2424 Size: 83 94 492 6199 Fragments arranged by size: 6199 492 94 83 PmeI GTTT'AAAC Cuts at: 4172 4172 Size: 6868 PmlI CAC'GTG Cuts at: 2168 2168 Size: 6868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4937 4969 6450 6482 4937 Size: 32 1481 32 5323 Fragments arranged by size: 5323 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2092 Size: 6868 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6868 PsiI TTA'TAA Cuts at: 4438 4438 Size: 6868 PspOMI G'GGCC_C Cuts at: 2133 2646 3491 2133 Size: 513 845 5510 Fragments arranged by size: 5510 845 513 PstI C_TGCA'G Cuts at: 830 3336 4143 4220 830 Size: 2506 807 77 3478 Fragments arranged by size: 3478 2506 807 77 PvuI CG_AT'CG Cuts at: 2001 2682 2001 Size: 681 6187 PvuII CAG'CTG Cuts at: 2959 3316 3625 2959 Size: 357 309 6202 Fragments arranged by size: 6202 357 309 SacI G_AGCT'C Cuts at: 2292 2643 2853 3129 3966 2292 Size: 351 210 276 837 5194 Fragments arranged by size: 5194 837 351 276 210 SalI G'TCGA_C Cuts at: 1936 4210 1936 Size: 2274 4594 SapI GCTCTTCn'nnn_ Cuts at: 2369 2441 2369 Size: 72 6796 SbfI CC_TGCA'GG Cuts at: 4220 4220 Size: 6868 ScaI AGT'ACT Cuts at: 5116 6828 5116 Size: 1712 5156 SexAI A'CCwGG_T Cuts at: 3154 3154 Size: 6868 SfcI C'TryA_G Cuts at: 826 1045 2368 3332 4139 4216 5351 6032 6223 Size: 219 1323 964 807 77 1135 681 191 Cuts at: 6223 826 Size: 1471 Fragments arranged by size: 1471 1323 1135 964 807 681 219 191 77 SfoI GGC'GCC Cuts at: 2021 2021 Size: 6868 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6868 SmaI CCC'GGG Cuts at: 1872 2027 2139 2220 2233 2919 4195 1872 Size: 155 112 81 13 686 1276 4545 Fragments arranged by size: 4545 1276 686 155 112 81 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 3008 4070 4972 5873 6135 Size: 197 926 549 516 1062 902 901 262 Cuts at: 6135 820 Size: 1553 Fragments arranged by size: 1553 1062 926 902 901 549 516 262 197 SnaBI TAC'GTA Cuts at: 493 4659 493 Size: 4166 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6868 SphI G_CATG'C Cuts at: 1132 4226 1132 Size: 3094 3774 SspI AAT'ATT Cuts at: 5 52 4792 5 Size: 47 4740 2081 Fragments arranged by size: 4740 2081 47 StuI AGG'CCT Cuts at: 1517 3434 3983 1517 Size: 1917 549 4402 Fragments arranged by size: 4402 1917 549 StyI C'CwwG_G Cuts at: 513 1065 2751 3321 3528 3708 4105 4307 513 Size: 552 1686 570 207 180 397 202 3074 Fragments arranged by size: 3074 1686 570 552 397 207 202 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 3802 4895 4912 5065 5250 5695 Size: 1255 1092 1130 1093 17 153 185 445 Cuts at: 5695 325 Size: 1498 Fragments arranged by size: 1498 1255 1130 1093 1092 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5114 6826 96 Size: 274 80 33 51 1443 3137 1712 138 Fragments arranged by size: 3137 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2084 2116 2231 2263 2373 2405 2810 2842 3335 Size: 32 115 32 110 32 405 32 493 Cuts at: 3335 3367 6828 6860 2084 Size: 32 3461 32 2092 Fragments arranged by size: 3461 2092 493 405 115 110 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6868 XbaI T'CTAG_A Cuts at: 4204 4204 Size: 6868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 3152 3622 2422 Size: 730 470 5668 Fragments arranged by size: 5668 730 470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6868 XmaI C'CCGG_G Cuts at: 1870 2025 2137 2218 2231 2917 4193 1870 Size: 155 112 81 13 686 1276 4545 Fragments arranged by size: 4545 1276 686 155 112 81 13 XmnI GAAnn'nnTTC Cuts at: 4997 4997 Size: 6868 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2338 4676 276 Size: 53 83 186 936 804 2338 2468 Fragments arranged by size: 2468 2338 936 804 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI