(Linear) (Six-base) MAPSORT of: KIEE6713.seq Check: 9326 from: 1 to: 2163 KIEE6713.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AatII G_ACGT'C Cuts at: 0 335 2163 Size: 335 1828 Acc65I G'GTAC_C Cuts at: 0 704 1754 2163 Size: 704 1050 409 Fragments arranged by size: 1050 704 409 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1671 1734 2163 Size: 1671 63 429 Fragments arranged by size: 1671 429 63 AfeI AGC'GCT Cuts at: 0 979 2163 Size: 979 1184 AflIII A'CryG_T Cuts at: 0 162 2163 Size: 162 2001 AleI CACnn'nnGTG Cuts at: 0 343 769 1300 2163 Size: 343 426 531 863 Fragments arranged by size: 863 531 426 343 AlwNI CAG_nnn'CTG Cuts at: 0 737 930 1176 1355 1413 2163 Size: 737 193 246 179 58 750 Fragments arranged by size: 750 737 246 193 179 58 ApaI G_GGCC'C Cuts at: 0 132 645 1490 2163 Size: 132 513 845 673 Fragments arranged by size: 845 673 513 132 ApaLI G'TGCA_C Cuts at: 0 1234 2163 Size: 1234 929 ApoI r'AATT_y Cuts at: 0 1060 2074 2163 Size: 1060 1014 89 Fragments arranged by size: 1060 1014 89 AvaI C'yCGr_G Cuts at: 0 8 20 132 213 226 912 1769 1972 Size: 8 12 112 81 13 686 857 203 Cuts at: 1972 2156 2163 Size: 184 7 Fragments arranged by size: 857 686 203 184 112 81 13 12 8 7 BamHI G'GATC_C Cuts at: 0 506 2163 Size: 506 1657 BanI G'GyrC_C Cuts at: 0 14 447 704 1656 1754 1863 2163 Size: 14 433 257 952 98 109 300 Fragments arranged by size: 952 433 300 257 109 98 14 BanII G_rGCy'C Cuts at: 0 132 287 638 645 848 1124 1490 1961 Size: 132 155 351 7 203 276 366 471 Cuts at: 1961 2152 2163 Size: 191 11 Fragments arranged by size: 471 366 351 276 203 191 155 132 11 7 BbeI G_GCGC'C Cuts at: 0 18 2163 Size: 18 2145 BbsI GAAGACnn'nnnn_ Cuts at: 0 1202 1542 2163 Size: 1202 340 621 Fragments arranged by size: 1202 621 340 BclI T'GATC_A Cuts at: 0 899 2163 Size: 899 1264 BglI GCCn_nnn'nGGC Cuts at: 0 12 117 2163 Size: 12 105 2046 Fragments arranged by size: 2046 105 12 BlpI GC'TnA_GC Cuts at: 0 575 1157 1403 2163 Size: 575 582 246 760 Fragments arranged by size: 760 582 575 246 Bme1580I G_kGCm'C Cuts at: 0 132 645 1135 1238 1490 1738 2163 Size: 132 513 490 103 252 248 425 Fragments arranged by size: 513 490 425 252 248 132 103 BmgBI CAC'GTC Cuts at: 0 1458 2163 Size: 1458 705 BmrI ACTGGGnnnn_n' Cuts at: 0 554 2163 Size: 554 1609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 442 1111 2016 2041 2163 Size: 306 136 669 905 25 122 Fragments arranged by size: 905 669 306 136 122 25 Bpu10I CC'TnA_GC Cuts at: 0 850 1811 2163 Size: 850 961 352 Fragments arranged by size: 961 850 352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 988 2050 2163 Size: 472 516 1062 113 Fragments arranged by size: 1062 516 472 113 BsaI GGTCTCn'nnnn_ Cuts at: 0 203 504 1663 2163 Size: 203 301 1159 500 Fragments arranged by size: 1159 500 301 203 BsaAI yAC'GTr Cuts at: 0 163 1857 2163 Size: 163 1694 306 Fragments arranged by size: 1694 306 163 BsaHI Gr'CG_yC Cuts at: 0 15 332 401 994 2163 Size: 15 317 69 593 1169 Fragments arranged by size: 1169 593 317 69 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 226 256 368 398 805 835 2163 Size: 226 30 112 30 407 30 1328 Fragments arranged by size: 1328 407 226 112 30 30 30 BseYI C'CCAG_C Cuts at: 0 27 809 1135 1307 1620 1626 2127 2163 Size: 27 782 326 172 313 6 501 36 Fragments arranged by size: 782 501 326 313 172 36 27 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 943 970 1368 2163 Size: 364 579 27 398 795 Fragments arranged by size: 795 579 398 364 27 BsiEI CG_ry'CG Cuts at: 0 677 2163 Size: 677 1486 BsiHKAI G_wGCw'C Cuts at: 0 287 638 848 911 1124 1238 1961 2163 Size: 287 351 210 63 213 114 723 202 Fragments arranged by size: 723 351 287 213 210 202 114 63 BsmBI CGTCTCn'nnnn_ Cuts at: 0 323 418 2163 Size: 323 95 1745 Fragments arranged by size: 1745 323 95 Bsp1286I G_dGCh'C Cuts at: 0 132 287 638 645 848 911 1124 1135 Size: 132 155 351 7 203 63 213 11 Cuts at: 1135 1238 1490 1738 1961 2152 2163 Size: 103 252 248 223 191 11 Fragments arranged by size: 351 252 248 223 213 203 191 155 132 103 63 11 11 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1115 2163 Size: 1115 1048 BsrBI CCG'CTC Cuts at: 0 124 153 242 2030 2163 Size: 124 29 89 1788 133 Fragments arranged by size: 1788 133 124 89 29 BsrDI GCAATG_nn' Cuts at: 0 1837 2163 Size: 1837 326 BsrFI r'CCGG_y Cuts at: 0 220 791 1322 1634 1659 2163 Size: 220 571 531 312 25 504 Fragments arranged by size: 571 531 504 312 220 25 BssSI C'ACGA_G Cuts at: 0 1759 1868 2163 Size: 1759 109 295 Fragments arranged by size: 1759 295 109 BstAPI GCAn_nnn'nTGC Cuts at: 0 930 1242 1413 2163 Size: 930 312 171 750 Fragments arranged by size: 930 750 312 171 BstXI CCAn_nnnn'nTGG Cuts at: 0 870 1374 2163 Size: 870 504 789 Fragments arranged by size: 870 789 504 BstYI r'GATC_y Cuts at: 0 506 2163 Size: 506 1657 Bsu36I CC'TnA_GG Cuts at: 0 1600 2163 Size: 1600 563 BtgI C'CryG_G Cuts at: 0 404 1316 2100 2163 Size: 404 912 784 63 Fragments arranged by size: 912 784 404 63 BtsI GCAGTG_nn' Cuts at: 0 376 2163 Size: 376 1787 DraIII CAC_nnn'GTG Cuts at: 0 393 1153 1242 1932 2163 Size: 393 760 89 690 231 Fragments arranged by size: 760 690 393 231 89 DrdI GACnn_nn'nnGTC Cuts at: 0 607 2163 Size: 607 1556 EaeI y'GGCC_r Cuts at: 0 296 407 971 1838 2163 Size: 296 111 564 867 325 Fragments arranged by size: 867 564 325 296 111 EarI CTCTTCn'nnn_ Cuts at: 0 364 436 2163 Size: 364 72 1727 Fragments arranged by size: 1727 364 72 EcoICRI GAG'CTC Cuts at: 0 285 636 846 1122 1959 2163 Size: 285 351 210 276 837 204 Fragments arranged by size: 837 351 285 276 210 204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 442 1111 1671 1734 2016 2041 2163 Size: 306 136 669 560 63 282 25 122 Fragments arranged by size: 669 560 306 282 136 122 63 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 2156 2163 Size: 2156 7 EcoO109I rG'GnC_Cy Cuts at: 0 87 128 129 642 734 1173 1486 1487 Size: 87 41 1 513 92 439 313 1 Cuts at: 1487 1533 1604 1815 2061 2163 Size: 46 71 211 246 102 Fragments arranged by size: 513 439 313 246 211 102 92 87 71 46 41 1 1 EcoRI G'AATT_C Cuts at: 0 1060 2074 2163 Size: 1060 1014 89 Fragments arranged by size: 1060 1014 89 FseI GG_CCGG'CC Cuts at: 0 224 2163 Size: 224 1939 HaeII r_GCGC'y Cuts at: 0 18 28 105 981 2163 Size: 18 10 77 876 1182 Fragments arranged by size: 1182 876 77 18 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 320 352 2163 Size: 320 32 1811 Fragments arranged by size: 1811 320 32 HincII GTy'rAC Cuts at: 0 1503 2163 Size: 1503 660 HindIII A'AGCT_T Cuts at: 0 1033 2163 Size: 1033 1130 KasI G'GCGC_C Cuts at: 0 14 2163 Size: 14 2149 KpnI G_GTAC'C Cuts at: 0 708 1758 2163 Size: 708 1050 405 Fragments arranged by size: 1050 708 405 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 269 1316 2163 Size: 269 1047 847 Fragments arranged by size: 1047 847 269 MscI TGG'CCA Cuts at: 0 298 409 2163 Size: 298 111 1754 Fragments arranged by size: 1754 298 111 MslI CAynn'nnrTG Cuts at: 0 343 442 769 1300 2163 Size: 343 99 327 531 863 Fragments arranged by size: 863 531 343 327 99 MspA1I CmG'CkG Cuts at: 0 954 1311 1620 1799 2163 Size: 954 357 309 179 364 Fragments arranged by size: 954 364 357 309 179 NaeI GCC'GGC Cuts at: 0 222 1636 1661 2163 Size: 222 1414 25 502 Fragments arranged by size: 1414 502 222 25 NarI GG'CG_CC Cuts at: 0 15 2163 Size: 15 2148 NcoI C'CATG_G Cuts at: 0 1316 2100 2163 Size: 1316 784 63 Fragments arranged by size: 1316 784 63 NgoMIV G'CCGG_C Cuts at: 0 220 1634 1659 2163 Size: 220 1414 25 504 Fragments arranged by size: 1414 504 220 25 NspI r_CATG'y Cuts at: 0 295 1057 1249 2163 Size: 295 762 192 914 Fragments arranged by size: 914 762 295 192 PflMI CCAn_nnn'nTGG Cuts at: 0 1932 2163 Size: 1932 231 PfoI T'CCnGG_A Cuts at: 0 419 502 596 1088 2163 Size: 419 83 94 492 1075 Fragments arranged by size: 1075 492 419 94 83 PmlI CAC'GTG Cuts at: 0 163 2163 Size: 163 2000 PpuMI rG'GwC_Cy Cuts at: 0 87 2163 Size: 87 2076 PspOMI G'GGCC_C Cuts at: 0 128 641 1486 2163 Size: 128 513 845 677 Fragments arranged by size: 845 677 513 128 PstI C_TGCA'G Cuts at: 0 1331 2138 2163 Size: 1331 807 25 Fragments arranged by size: 1331 807 25 PvuI CG_AT'CG Cuts at: 0 677 2163 Size: 677 1486 PvuII CAG'CTG Cuts at: 0 954 1311 1620 2163 Size: 954 357 309 543 Fragments arranged by size: 954 543 357 309 SacI G_AGCT'C Cuts at: 0 287 638 848 1124 1961 2163 Size: 287 351 210 276 837 202 Fragments arranged by size: 837 351 287 276 210 202 SapI GCTCTTCn'nnn_ Cuts at: 0 364 436 2163 Size: 364 72 1727 Fragments arranged by size: 1727 364 72 SexAI A'CCwGG_T Cuts at: 0 1149 2163 Size: 1149 1014 SfcI C'TryA_G Cuts at: 0 363 1327 2134 2163 Size: 363 964 807 29 Fragments arranged by size: 964 807 363 29 SfoI GGC'GCC Cuts at: 0 16 2163 Size: 16 2147 SmaI CCC'GGG Cuts at: 0 22 134 215 228 914 2163 Size: 22 112 81 13 686 1249 Fragments arranged by size: 1249 686 112 81 22 13 SmlI C'TyrA_G Cuts at: 0 487 1003 2065 2163 Size: 487 516 1062 98 Fragments arranged by size: 1062 516 487 98 StuI AGG'CCT Cuts at: 0 1429 1978 2163 Size: 1429 549 185 Fragments arranged by size: 1429 549 185 StyI C'CwwG_G Cuts at: 0 746 1316 1523 1703 2100 2163 Size: 746 570 207 180 397 63 Fragments arranged by size: 746 570 397 207 180 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 667 1797 2163 Size: 667 1130 366 Fragments arranged by size: 1130 667 366 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 79 111 226 258 368 400 805 837 Size: 79 32 115 32 110 32 405 32 Cuts at: 837 1330 1362 2163 Size: 493 32 801 Fragments arranged by size: 801 493 405 115 110 79 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 417 1147 1617 2163 Size: 417 730 470 546 Fragments arranged by size: 730 546 470 417 XmaI C'CCGG_G Cuts at: 0 20 132 213 226 912 2163 Size: 20 112 81 13 686 1251 Fragments arranged by size: 1251 686 112 81 20 13 ZraI GAC'GTC Cuts at: 0 333 2163 Size: 333 1830 Enzymes that do cut: AatII Acc65I AcuI AfeI AflIII AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI PstI PvuI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI BglII BmtI BplI BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoRV FalI FspI FspAI HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI