################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaII AviII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI Ksp632I KspI MamI MluI MroI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 367 GGTACC G^GTACC Asp718I 367 GGTACC G^GTACC AspHI 217 GTGCTC G[AT]GC[AT]^C AvaI 911 CCCGAG C^[CT]CG[GA]G AvrII 429 CCTAGG C^CTAGG BanI 367 GGTACC G^G[CT][GA]CC BbvI 322 GCTGC GCAGC(8/12) BglII 1055 AGATCT A^GATCT BlnI 429 CCTAGG C^CTAGG BsaI 609 GGTCTC GGTCTC(1/5) BsaWI 50 ACCGGA [AT]^CCGG[AT] BsiHKAI 217 GTGCTC G[AT]GC[AT]^C BsmFI 964 GTCCC GGGAC(10/14) BsmI 325 GCATTC GAATGC(1/-1) BsoBI 911 CCCGAG C^[CT]CG[GA]G BspHI 264 TCATGA T^CATGA BsrDI 817 GCAATG GCAATG(2/0) BstAPI 8 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 60 GGTCACC G^GT[ACGT]ACC BstPI 60 GGTCACC G^GT[ACGT]ACC BstYI 1055 AGATCT [GA]^GATC[CT] Cac8I 155 GCTGGC GC[ACGT]^[ACGT]GC DrdI 955 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 60 GGTCACC G^GT[ACGT]ACC EcoRV 1018 GATATC GAT^ATC Fnu4HI 322 GCTGC GC^[ACGT]GC HaeII 97 GGCGCT [GA]GCGC^[CT] HincII 906 GTTGAC GT[CT]^[GA]AC HindII 906 GTTGAC GT[CT]^[GA]AC HinfI 137 GAATC G^A[ACGT]TC ItaI 322 GCTGC GC^[ACGT]GC KpnI 367 GGTACC GGTAC^C MaeII 724 ACGT A^CGT MfeI 356 CAATTG C^AATTG MflI 1055 AGATCT [GA]^GATC[CT] MunI 356 CAATTG C^AATTG NspI 149 ACATGC [GA]CATG^[CT] PshAI 694 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 264 TCATGA T^CATGA SfcI 865 CTACAG C^T[GA][CT]AG StuI 432 AGGCCT AGG^CCT TfiI 137 GAATC G^A[AT]TC TseI 322 GCTGC G^C[AT]GC XhoII 1055 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 948 GAATTT [GA]^AATT[CT] 970 GAATTT ApoI 948 GAATTT [GA]^AATT[CT] 970 GAATTT BalI 72 TGGCCA TGG^CCA 572 TGGCCA BcnI 482 CCCGG CC^[GC]GG 606 CCGGG BmyI 217 GTGCTC G[AGT]GC[ACT]^C 828 GGGCAC BseRI 29 CTCCTC GAGGAG(10/8) 980 CTCCTC BsiYI 51 CCGGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 599 CCAGTGTCCGG BslI 51 CCGGAGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 599 CCAGTGTCCGG BsmAI 343 GAGAC GTCTC(1/5) 610 GTCTC Bsp1286I 217 GTGCTC G[AGT]GC[ACT]^C 828 GGGCAC BstNI 339 CCTGG CC^[AT]GG 1014 CCTGG BstXI 672 CCACCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1023 CCACATCAATGG CfoI 98 GCGC GCG^C 153 GCGC EcoRII 339 CCTGG ^CC[AT]GG 1014 CCTGG EcoT14I 65 CCAAGG C^C[AT][AT]GG 429 CCTAGG HhaI 98 GCGC GCG^C 153 GCGC HinP1I 98 GCGC G^CGC 153 GCGC MluNI 72 TGGCCA TGG^CCA 572 TGGCCA MscI 72 TGGCCA TGG^CCA 572 TGGCCA MspA1I 320 CAGCTG C[AC]G^C[GT]G 614 CAGCTG MvaI 339 CCTGG CC^[AT]GG 1014 CCTGG NciI 482 CCCGG CC^[GC]GG 606 CCGGG NlaIV 367 GGTACC GG[ACGT]^[ACGT]CC 916 GGTTCC PvuII 320 CAGCTG CAG^CTG 614 CAGCTG StyI 65 CCAAGG C^C[AT][AT]GG 429 CCTAGG TaqI 123 TCGA T^CGA 495 TCGA Tsp45I 61 GTCAC ^GT[GC]AC 386 GTGAC TthHB8I 123 TCGA T^CGA 495 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI BfaI BpmI BsaJI BsrI BssKI BstF5I DdeI DpnII EaeI FokI HaeIII HapII HpaII HphI MaeI MaeIII MboI MboII MnlI MslI MspI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI Tsp509I TspRI