(Circular) (Six-base) MAPSORT of: pFN21AE5515.seq Check: 1386 from: 1 to: 5785 pFN21AE5515 5785 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 2372 3106 2372 Size: 734 5051 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3848 5232 5500 1632 Size: 2216 1384 268 1917 Fragments arranged by size: 2216 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 3528 5100 3528 Size: 1572 4213 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2953 2975 3094 3320 3472 5581 1493 Size: 1460 22 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 119 22 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2916 3110 1870 Size: 73 973 194 4545 Fragments arranged by size: 4545 973 194 73 AvrII C'CTAG_G Cuts at: 2434 2434 Size: 5785 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2372 3106 4561 618 Size: 325 217 627 585 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 585 325 217 BanII G_rGCy'C Cuts at: 1391 3104 1391 Size: 1713 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3060 5780 3060 Size: 2720 3065 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2837 3846 5002 1790 Size: 1047 1009 1156 2573 Fragments arranged by size: 2573 1156 1047 1009 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 3516 4474 Size: 416 53 83 399 24 962 1137 958 Cuts at: 4474 442 Size: 1753 Fragments arranged by size: 1753 1137 962 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2759 2859 4445 1239 Size: 1127 393 100 1586 2579 Fragments arranged by size: 2579 1586 1127 393 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2620 4448 882 Size: 617 1121 1828 2219 Fragments arranged by size: 2219 1828 1121 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 4218 4616 4890 5037 5448 2055 Size: 2163 398 274 147 411 2392 Fragments arranged by size: 2392 2163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2415 2445 2723 2753 1178 Size: 30 165 30 1012 30 278 30 4210 Fragments arranged by size: 4210 1012 278 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2976 1186 Size: 839 951 3995 Fragments arranged by size: 3995 951 839 BseYI C'CCAG_C Cuts at: 4988 5484 4988 Size: 496 5289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 3846 3931 5002 2226 Size: 1620 85 1071 3009 Fragments arranged by size: 3009 1620 1071 85 BsmI GAATG_Cn' Cuts at: 2330 3296 3389 2330 Size: 966 93 4726 Fragments arranged by size: 4726 966 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2837 3104 3846 3931 5002 1391 Size: 399 436 611 267 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 436 399 267 85 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 2269 3674 5404 2269 Size: 1405 1730 2650 Fragments arranged by size: 2650 1730 1405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3140 844 Size: 2296 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 4280 4454 65 Size: 1183 1581 1451 174 1396 Fragments arranged by size: 1581 1451 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2019 794 Size: 1225 4560 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 2065 1184 Size: 881 4904 BstXI CCAn_nnnn'nTGG Cuts at: 2684 3035 2684 Size: 351 5434 BstYI r'GATC_y Cuts at: 1972 3060 3115 3482 3867 3884 5336 5422 5434 Size: 1088 55 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1088 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2226 3301 4095 4115 2226 Size: 1075 794 20 3896 Fragments arranged by size: 3896 1075 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2966 4792 809 Size: 507 1650 1826 1802 Fragments arranged by size: 1826 1802 1650 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2489 2577 Size: 54 1425 50 275 18 247 412 88 Cuts at: 2577 3232 4121 5750 8 Size: 655 889 1629 43 Fragments arranged by size: 1629 1425 889 655 412 275 247 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3715 1353 Size: 2362 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2759 2859 3848 4445 5232 5500 Size: 393 734 393 100 989 597 787 268 Cuts at: 5500 1239 Size: 1524 Fragments arranged by size: 1524 989 787 734 597 393 393 268 100 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 EcoRV GAT'ATC Cuts at: 3025 3025 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2106 4932 1678 Size: 428 2826 2531 Fragments arranged by size: 2826 2531 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373 Size: 32 135 32 2854 32 42 32 2626 Fragments arranged by size: 2854 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3129 3375 669 Size: 988 281 975 216 246 3079 Fragments arranged by size: 3079 988 975 281 246 216 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 2376 3110 2376 Size: 734 5051 MfeI C'AATT_G Cuts at: 2361 3384 2361 Size: 1023 4762 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2147 3005 4899 5083 1305 Size: 842 858 1894 184 2007 Fragments arranged by size: 2007 1894 858 842 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2079 2579 10 Size: 54 1425 343 247 500 3216 Fragments arranged by size: 3216 1425 500 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 2426 2547 2682 3033 3743 4102 4261 Size: 648 1260 121 135 351 710 359 159 Cuts at: 4261 518 Size: 2042 Fragments arranged by size: 2042 1260 710 648 359 351 159 135 121 MspA1I CmG'CkG Cuts at: 1606 2327 2621 3201 3878 5026 5271 1606 Size: 721 294 580 677 1148 245 2120 Fragments arranged by size: 2120 1148 721 677 580 294 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 2158 3143 4688 1132 Size: 97 929 985 1545 2229 Fragments arranged by size: 2229 1545 985 929 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2703 1613 Size: 1090 4695 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2327 2621 2327 Size: 294 5491 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2870 3133 4268 4949 5140 826 Size: 219 1825 263 1135 681 191 1471 Fragments arranged by size: 1825 1471 1135 681 263 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 2439 1517 Size: 922 4863 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2434 3224 513 Size: 552 939 66 364 790 3074 Fragments arranged by size: 3074 939 790 552 364 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2623 4031 5743 Size: 274 80 33 51 1443 646 1408 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1712 1443 1408 646 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI