################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI Eco47III EcoNI EcoO109I EcoO65I EcoRV FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NarI NciI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AhdI 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 769 GGATC GGATC(4/5) AocI 688 CCTGAGG CC^T[ACGT]AGG AseI 1095 ATTAAT AT^TAAT AsnI 1095 ATTAAT AT^TAAT Asp700I 1062 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 343 GGACC G^G[AT]CC BbsI 543 GTCTTC GAAGAC(2/6) BclI 1166 TGATCA T^GATCA BmyI 1012 GGGCAC G[AGT]GC[ACT]^C BpmI 303 CTGGAG CTGGAG(16/14) BpuAI 543 GTCTTC GAAGAC(2/6) BsaI 100 GAGACC GGTCTC(1/5) BsaWI 156 ACCGGA [AT]^CCGG[AT] BseRI 979 GAGGAG GAGGAG(10/8) BsiEI 41 CGGCCG CG[GA][CT]^CG Bsp1286I 1012 GGGCAC G[AGT]GC[ACT]^C BspHI 488 TCATGA T^CATGA BsrFI 39 GCCGGC [GA]^CCGG[CT] BstYI 769 GGATCT [GA]^GATC[CT] Bsu36I 688 CCTGAGG CC^T[ACGT]AGG Cfr10I 39 GCCGGC [GA]^CCGG[CT] DsaI 418 CCATGG C^C[GA][CT]GG EaeI 41 CGGCCG [CT]^GGCC[GA] EagI 41 CGGCCG C^GGCCG Eam1105I 1028 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 41 CGGCCG C^GGCCG Eco52I 41 CGGCCG C^GGCCG Eco81I 688 CCTGAGG CC^T[ACGT]AGG EcoRI 1062 GAATTC G^AATTC EcoT14I 418 CCATGG C^C[AT][AT]GG EcoT22I 1256 ATGCAT ATGCA^T FauI 905 CCCGC CCCGC(4/6) FbaI 1166 TGATCA T^GATCA HgaI 22 GACGC GACGC(5/10) MflI 769 GGATCT [GA]^GATC[CT] MslI 787 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 115 CAGCGG C[AC]G^C[GT]G MwoI 224 GCACTTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 39 GCCGGC GCC^GGC NcoI 418 CCATGG C^CATGG NgoMIV 39 GCCGGC G^CCGGC NsiI 1256 ATGCAT ATGCA^T PflMI 1002 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1095 ATTAAT AT^TAAT RcaI 488 TCATGA T^CATGA SapI 779 GCTCTTC GCTCTTC(1/4) ScaI 857 AGTACT AGT^ACT StyI 418 CCATGG C^C[AT][AT]GG Van91I 1002 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 882 CCAGGAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 769 GGATCT [GA]^GATC[CT] XmnI 1062 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BbvI 69 GCTGC GCAGC(8/12) 1120 GCTGC BsaJI 368 CCCTGG C^C[ACGT][ACGT]GG 418 CCATGG BsmFI 1026 GGGAC GGGAC(10/14) 366 GTCCC BsrDI 791 GCAATG GCAATG(2/0) 908 GCAATG Cac8I 39 GCCGGC GC[ACGT]^[ACGT]GC 334 GCTGGC Cfr13I 62 GGGCC G^G[ACGT]CC 343 GGACC EarI 780 CTCTTC CTCTTC(1/4) 853 GAAGAG HapII 40 CCGG C^CGG 157 CCGG HindIII 697 AAGCTT A^AGCTT 1400 AAGCTT HpaII 40 CCGG C^CGG 157 CCGG HphI 577 GGTGA GGTGA(8/7) 1189 TCACC Ksp632I 780 CTCTTC CTCTTC(1/4) 853 GAAGAG MspI 40 CCGG C^CGG 157 CCGG NlaIV 61 GGGGCC GG[ACGT]^[ACGT]CC 900 GGAACC Sau96I 62 GGGCC G^G[ACGT]CC 343 GGACC TaqI 201 TCGA T^CGA 1104 TCGA TseI 69 GCTGC G^C[AT]GC 1120 GCTGC TthHB8I 201 TCGA T^CGA 1104 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI BfaI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I TspRI