(Circular) (Six-base) MAPSORT of: pFN21AE4458.seq Check: 6636 from: 1 to: 6130 pFN21AE4458 6130 bp With 182 enzymes: SgfI * March 28, 2012 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 4193 5577 5845 1632 Size: 654 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 3427 5029 6006 1203 Size: 2224 1602 977 1327 Fragments arranged by size: 2224 1602 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 3038 4859 3038 Size: 1821 4309 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2555 1184 Size: 1371 4759 AlwNI CAG_nnn'CTG Cuts at: 2241 2264 2374 3873 5445 2241 Size: 23 110 1499 1572 2926 Fragments arranged by size: 2926 1572 1499 110 23 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2513 2678 3067 3439 3665 3817 5926 1493 Size: 1020 165 389 372 226 152 2109 1697 Fragments arranged by size: 2109 1697 1020 389 372 226 165 152 AseI AT'TA_AT Cuts at: 160 1032 3101 4684 160 Size: 872 2069 1583 1606 Fragments arranged by size: 2069 1606 1583 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2139 2172 2139 Size: 33 6097 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3449 1391 Size: 2058 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2541 928 Size: 971 642 4517 Fragments arranged by size: 4517 971 642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 3171 1548 Size: 1623 4507 BfrBI ATG'CAT Cuts at: 3263 3263 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 3021 4191 5347 1790 Size: 1231 1170 1156 2573 Fragments arranged by size: 2573 1231 1170 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3023 3861 4819 Size: 416 53 83 399 24 1606 838 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1606 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2329 4790 1239 Size: 1090 2461 2579 Fragments arranged by size: 2579 2461 1090 Bpu10I CC'TnA_GC Cuts at: 3233 3233 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 4793 882 Size: 617 600 2694 2219 Fragments arranged by size: 2694 2219 617 600 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2161 4563 4961 5235 5382 5793 2161 Size: 2402 398 274 147 411 2498 Fragments arranged by size: 2498 2402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2999 1186 Size: 1813 4317 BseYI C'CCAG_C Cuts at: 5333 5829 5333 Size: 496 5634 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3580 4341 5369 1540 Size: 275 186 48 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 4191 4276 5347 4191 Size: 85 1071 4974 Fragments arranged by size: 4974 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3021 3449 4191 4276 5347 1391 Size: 399 1231 428 742 85 1071 2174 Fragments arranged by size: 2174 1231 1071 742 428 399 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 2493 4019 5749 2493 Size: 1526 1730 2874 Fragments arranged by size: 2874 1730 1526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2803 2920 4625 4799 65 Size: 1183 1555 117 1705 174 1396 Fragments arranged by size: 1705 1555 1396 1183 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4774 4961 1700 Size: 110 234 2730 187 2869 Fragments arranged by size: 2869 2730 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2774 3460 3827 4212 4229 5681 5767 5779 Size: 802 686 367 385 17 1452 86 12 Cuts at: 5779 6125 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 802 686 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2694 2694 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2004 2423 513 Size: 552 939 419 4220 Fragments arranged by size: 4220 939 552 419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2004 487 Size: 683 56 778 4613 Fragments arranged by size: 4613 778 683 56 BtsI GCAGTG_nn' Cuts at: 3386 3646 4440 4460 3386 Size: 260 794 20 5056 Fragments arranged by size: 5056 794 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3142 3177 529 Size: 35 2578 35 3482 Fragments arranged by size: 3482 2578 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3577 4466 Size: 54 1425 50 275 18 216 1531 889 Cuts at: 4466 6095 8 Size: 1629 43 Fragments arranged by size: 1629 1531 1425 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3577 1537 Size: 275 234 1531 4090 Fragments arranged by size: 4090 1531 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 2853 4060 1353 Size: 1438 62 1207 3423 Fragments arranged by size: 3423 1438 1207 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2329 4193 4790 5577 5845 1239 Size: 393 654 43 1864 597 787 268 1524 Fragments arranged by size: 1864 1524 787 654 597 393 268 43 EcoRI G'AATT_C Cuts at: 3067 3439 3817 3067 Size: 372 378 5380 Fragments arranged by size: 5380 378 372 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2291 2077 Size: 214 5916 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 3024 3056 3156 Size: 32 135 32 508 32 912 32 100 Cuts at: 3156 3188 4771 4803 4845 4877 1373 Size: 32 1583 32 42 32 2626 Fragments arranged by size: 2626 1583 912 508 135 100 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 2702 3405 748 Size: 1954 703 3473 Fragments arranged by size: 3473 1954 703 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2796 4088 4447 4606 518 Size: 648 843 787 1292 359 159 2042 Fragments arranged by size: 2042 1292 843 787 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 3546 4223 5371 5616 1606 Size: 516 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 516 245 NaeI GCC'GGC Cuts at: 2046 2046 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2004 2423 513 Size: 552 939 419 4220 Fragments arranged by size: 4220 939 552 419 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2044 2044 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 3265 3265 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 3013 1108 Size: 1905 4225 PfoI T'CCnGG_A Cuts at: 2084 2886 2084 Size: 802 5328 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 2436 3700 2436 Size: 1264 4866 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 2791 2791 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 2864 4378 6090 2864 Size: 1514 1712 2904 Fragments arranged by size: 2904 1712 1514 SfcI C'TryA_G Cuts at: 826 1045 3478 4613 5294 5485 826 Size: 219 2433 1135 681 191 1471 Fragments arranged by size: 2433 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 2423 3569 513 Size: 552 939 419 1146 3074 Fragments arranged by size: 3074 1146 939 552 419 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4157 4174 4327 4512 4957 325 Size: 1255 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2862 2869 2998 Size: 274 80 33 51 1443 885 7 129 Cuts at: 2998 4376 6088 96 Size: 1378 1712 138 Fragments arranged by size: 1712 1443 1378 885 274 138 129 80 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 6090 6122 2018 Size: 32 4040 32 2026 Fragments arranged by size: 4040 2026 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2894 2894 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 3071 4259 3071 Size: 1188 4942 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI